<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Oxamyl is a carbamate insecticide used to control a broad spectrum of insects. It can also affect non-targeted organisms when applied incorrectly. The world food production depends partially on honeybee pollination abilities and therefore it is directly linked to the health of bees. The success of the colony development depends, among other factors, on the health of the larvae. The first 6 days are crucial for their development. In this stage, the worker larvae grow exponentially and may be exposed to xenobiotics via their diet. In this study, we investigated the effect of oxamyl on honeybee larvae (<italic>Apis mellifera</italic>) by monitoring the changes in their antioxidant enzyme system. The activities of superoxide dismutase, catalase and glutathione-S-transferase were determined in the homogenates of <italic>in vitro</italic> reared honeybee larvae after their single dietary exposure to oxamyl at doses of 1.25, 2.5, 5, 10 and 20 µg a.i./larva (a. i.—active ingredient). The doses of oxamyl did not cause statistically significant changes in the activities of the enzymes. Even a slight activation of these enzymes protected the larvae from the adverse effects of the reactive oxygen species (ROS). Marked changes in both the enzyme activity and the content of lipid peroxidation products were observed at the oxamyl dose of 10 µg a. i./larva. This fact may indicate a potential oxidative damage to the larvae. These results allowed us to assume that the toxic effects of oxamyl involves not only the inhibition of acetylcholine esterase but is also associated with ROS production.</p></abstract>

Oxamyl is a carbamate insecticide used to control a broad spectrum of insects. It can also affect non-targeted organisms when applied incorrectly. The world food production depends partially on honeybee pollination abilities and therefore it is directly linked to the health of bees. The success of the colony development depends, among other factors, on the health of the larvae. The first 6 days are crucial for their development. In this stage, the worker larvae grow exponentially and may be exposed to xenobiotics via their diet. In this study, we investigated the effect of oxamyl on honeybee larvae (Apis mellifera) by monitoring the changes in their antioxidant enzyme system. The activities of superoxide dismutase, catalase and glutathione-S-transferase were determined in the homogenates of in vitro reared honeybee larvae after their single dietary exposure to oxamyl at doses of 1.25, 2.5, 5, 10 and 20 µg a.i./larva (a. i.—active ingredient). The doses of oxamyl did not cause statistically significant changes in the activities of the enzymes. Even a slight activation of these enzymes protected the larvae from the adverse effects of the reactive oxygen species (ROS). Marked changes in both the enzyme activity and the content of lipid peroxidation products were observed at the oxamyl dose of 10 µg a. i./larva. This fact may indicate a potential oxidative damage to the larvae. These results allowed us to assume that the toxic effects of oxamyl involves not only the inhibition of acetylcholine esterase but is also associated with ROS production.

Antioxidant Enzymes of Honeybee Larvae Exposed to Oxamyl

University of Veterinary Medicine and Pharmacy in Košice

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Antioxidant Enzymes of Honeybee Larvae Exposed to Oxamyl"}

Oxamyl is a carbamate insecticide used to control a broad spectrum of insects. It can also affect non-targeted organisms when applied incorrectly. The world...

Antioxidant Enzymes of Honeybee Larvae Exposed to Oxamyl

Published Online: Dec 21, 2019

Page range: 9 - 14

Received: Jul 15, 2019

Accepted: Sep 19, 2019

DOI: https://doi.org/10.2478/fv-2019-0032

Keywords
antioxidant enzyme system, honeybee, larvae, oxamyl

© 2019 M. Prezenská et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Antioxidant Enzymes of Honeybee Larvae Exposed to Oxamyl

Published Online: Dec 21, 2019

Page range: 9 - 14

Received: Jul 15, 2019

Accepted: Sep 19, 2019

DOI: https://doi.org/10.2478/fv-2019-0032

Keywordsantioxidant enzyme system, honeybee, larvae, oxamyl

© 2019 M. Prezenská et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
antioxidant enzyme system, honeybee, larvae, oxamyl