1. bookVolume 62 (2018): Issue 3 (September 2018)
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Mastitis Pathogens Isolated from Raw Milk Samples on Sheep Farms Situated in Marginal Parts of Slovakia

Published Online: 16 Oct 2018
Volume & Issue: Volume 62 (2018) - Issue 3 (September 2018)
Page range: 56 - 61
Received: 19 Jul 2018
Accepted: 31 Aug 2018
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

Relatively large parts of the Slovak territory are marginal regions, which in terms of the economy of ruminant rearing can effectively produce animal commodities only occasionally. The aim of this study was to evaluate the occurrence and aetiology of mastitis during one milking season in two sheep dairy herds of 224 (A) and 270 (B) ewes situated in the marginal parts of Slovakia. A complex examination of health status of ewes based on: the clinical examination of the udder, macroscopic evaluation of milk with the California mastitis test (CMT) and the bacteriological analysis of raw milk samples from individual halves were carried out at the beginning (April) and at the end (September) of the milking season. The prevalence of intramammary infection (IMI) in the herds of ewes (A and B) were 19.1 % and 14.3 %, respectively. In both herds we confirmed predominantly the subclinical forms of IMI. The highest percentages of aetiological agents were determined for coagulase negative staphylococci (CoNS), such as Staphylococcus chromogenes, Staphylococcus schleiferi, Staphylococcus epidermidis, Staphylococcus xylosus, Staphylococcus piscifermentans and Staphylococcus intermedius, the occurrences of which were determined in 41 (48.2 %) and 37 (47.9 %) positive milk samples, respectively.

Keywords

1. Bergonier, D., Crémoux, R., Rupp, R., Lagriffoul, G., Erthelot, X., 2003: Mastitis of dairy small ruminants. Vet. Res., 34, 689—716.10.1051/vetres:2003030Search in Google Scholar

2. Contreras, A., Sierra, D., Sanchez, A., Corrales, J. C., Marcoc, J. C., Paape, M. J., Gonzalo, C., 2007: Mastitis in small ruminant. Small Rumin. Res., 68, 145—153.10.1016/j.smallrumres.2006.09.011Search in Google Scholar

3. Fthenakis, G. C., 1994: Prevalence and aetiology of subclinical mastitis in ewes of southern Greece. Small Rumin. Res., 13, 293—300.10.1016/0921-4488(94)90078-7Search in Google Scholar

4. Fthenakis, G. C., 1995: California mastitis test and Whiteside test in diagnosis of subclinical mastitis of dairy ewes. Small Rumin. Res., 16, 271—276.10.1016/0921-4488(95)00638-2Search in Google Scholar

5. Hariharan, H., Donachie, W., Macaldowie, C., Keefe, G., 2004: Bacteriology and somatic cell counts in milk samples from ewes on a Scottish farm. Can. J. Vet. Res., 68, 188—192.Search in Google Scholar

6. Hawari A. D., Obeidat, M., Awaisheh, S. Sh., Al-Daghistani, H. I., Al-Abbadi, A. A., Omar, S. S., et al., 2014: Prevalence of mastitis pathogens and their resistance against antimicrobial agents in Awassi sheep in Al-Balqa province of Jordan. Am. J. Anim. Vet. Sci., 9, 116—121.10.3844/ajavsp.2014.116.121Search in Google Scholar

7. Holečková, B., Kaliňačová, V., Gondol, J., Fotta, M., Holoda, E., Beličková, E., 2004: Production of enterotoxins by Staphylococcus aureus isolated from sheep milk. Bulletin of the Veterinary Institute in Pulawy, 48, 41—45.Search in Google Scholar

8. Idriss, S. H., Tančin, V., Foltýs, V., Kirchnerová, K., Tančinová, D., Vršková, M., 2013: Relationship between mastitis causative pathogens and somatic cell count in milk of dairy cows. Potravinárstvo (Food Industry), 7, 207—212.10.5219/304Search in Google Scholar

9. Kmeť, V., Bujnáková, D., 2018: Antimicrobial resistance Escherichia coli isolated from calves. Journal of Microbiology, Biotechnology and Food Sciences. 7, 412—415.10.15414/jmbfs.2018.7.4.412-415Search in Google Scholar

10. Lafi, S. Q., Hailat, N. Q., 1998: Bovine and ovine mastitis in Dhulia Valley of Jordan. Veterinary Archives, 68, 51—57.Search in Google Scholar

11. Malinowski, E., Lassa, H., Kłossowska, A., Smulski, S., Markiewicz, H., Kaczmarowski, M., 2006: Etiological agents of dairy cows’ mastitis in western part of Poland. Pol. J. Vet. Sci., 9, 191—194.Search in Google Scholar

12. Mørk T., Waage, S., Tollersrud, T., Kvitle, B., Sviland, S., 2007: Clinical mastitis in ewes; bacteriology, epidemiology and clinical features. Acta Veterinaria Scandinavica, 49, 23.10.1186/1751-0147-49-23204896817892567Search in Google Scholar

13. Narenji S. R., Mahdavi A., Moezifar, M., 2015: Prevalence and etiology of subclinical mastitis in dairy ewes in two seasons in Semnan province, Iran. Tropical Animal Health and Production, 47, 1249—1254.10.1007/s11250-015-0855-y26041046Search in Google Scholar

14. Ozenc, E., Seker, E., Baki Acar, D., Birdane, M. K., Darbaz I., Dogan, N., 2011: The importance of Staphylococci and threshold value of somatic cell count for diagnosis of subclinical mastitis in Pirlak sheep at mid-lactation. Reprod. Dom. Anim., 46, 970—974.10.1111/j.1439-0531.2011.01768.x21366722Search in Google Scholar

15. Spišiak, P., 1999: Agriculture in marginal areas (on the example of the White Carpathians) (In Slovak), Folia Geographica, 3, 198—203.Search in Google Scholar

16. Tančin, V., Bauer, M., Holko, I., Baranovič, Š., 2016: Etiology of mastitis in ewes and possible genetic and epigenetic factors involved. Slovak J. Anim. Sci., 49, 85—93.Search in Google Scholar

17. Tančin, V., Uhrinčať, M., 2014: The effect of somatic cells on milk yield and milk flow at quarter level. Veterinarija ir Zootechnika (Vet. Med. Zoot.), 66, 69—72.Search in Google Scholar

18. Thorberg, B. M., Kühn, I., Aarestrup, F. M., Brändström, B., Jonsson, P., Danielsson-Tham, M. L., 2006: Pheno- and genotyping of Staphylococcus epidermidis isolated from bovine milk and human skin. Vet. Microbiol., 115, 163—172.10.1016/j.vetmic.2006.01.01316530988Search in Google Scholar

19. Vautor, E., Cocckfield, J., Marechal, C., Loir, Y., Le Chevalier, M., Robinson, A. D., et al., 2009: Difference in virulence between Staphylococcus aureus isolates causing gangrenous mastitis versus subclinical mastitis in a dairy sheep flock. Vet. Res., 6, 40—56.10.1051/vetres/2009039273332819576164Search in Google Scholar

20. Vršková, M., Tančin, V., Kirchnerová, K., Sláma, P., 2015: Evaluation of daily milk production in Tsigaia ewes by somatic cell count. Potravinárstvo (Food Industry), 9, 206—210.10.5219/439Search in Google Scholar

21. Zadoks, R. N., Middleton, J. R., Mc Dougall, S., Katholm, J., Schukken, Y. H., 2011: Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. Journal of Mammary Gland Biology and Neoplasia, 16, 4, 357—72.10.1007/s10911-011-9236-y320883221968538Search in Google Scholar

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