<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A modified agyrophil technique was applied to peripheral blood smears to determine the mean AgNOR counts (MAC) of lymphocytes and ultimately assess the state of the lymphoid system in various clinical conditions of dogs. Fifty dogs, from clinically normal to pets with leukaemia, presented to the Veterinary Teaching Hospital, were recruited. Blood smears from each dog were stained with routine Romanowsky and modified agyrophil stains. Signalment, clinical diagnoses and hematologic parameters of the dogs were related to the MAC. An AgNOR proliferative index (AgPI) — percentage of lymphocytes with 3 or more AgNORs, was determined, and correlated with MAC. The statistical significance was determined at P &lt; 0.05. MAC ranged from 1.17 in clinically healthy patients to 6.00 in leukaemic patients. The MAC was 2.00 in patients (n = 26) with lymphocyte counts within reference intervals (900—2400 per microliter); 2.23 in patients (n = 4) with lymphopenia; 2.18 in patients with lymphocytosis (n = 18) and 4.73 in patients (n = 4) with lymphocytic leukemia. Also, the MAC was 2.00 in non-anemic dogs while it was 2.47, 2.49 and 3.06 in patients with mild, moderate and severe anaemia, respectively. The MAC correlated strongly with AgPI (r = 0.91). The ancillary AgNOR technique provides a cheaper, more rapid and sensitive tool than routine lymphocyte counts in assessing the state of lymphoid proliferation in a variety of conditions in the dog.</p></abstract>

A modified agyrophil technique was applied to peripheral blood smears to determine the mean AgNOR counts (MAC) of lymphocytes and ultimately assess the state of the lymphoid system in various clinical conditions of dogs. Fifty dogs, from clinically normal to pets with leukaemia, presented to the Veterinary Teaching Hospital, were recruited. Blood smears from each dog were stained with routine Romanowsky and modified agyrophil stains. Signalment, clinical diagnoses and hematologic parameters of the dogs were related to the MAC. An AgNOR proliferative index (AgPI) — percentage of lymphocytes with 3 or more AgNORs, was determined, and correlated with MAC. The statistical significance was determined at P < 0.05. MAC ranged from 1.17 in clinically healthy patients to 6.00 in leukaemic patients. The MAC was 2.00 in patients (n = 26) with lymphocyte counts within reference intervals (900—2400 per microliter); 2.23 in patients (n = 4) with lymphopenia; 2.18 in patients with lymphocytosis (n = 18) and 4.73 in patients (n = 4) with lymphocytic leukemia. Also, the MAC was 2.00 in non-anemic dogs while it was 2.47, 2.49 and 3.06 in patients with mild, moderate and severe anaemia, respectively. The MAC correlated strongly with AgPI (r = 0.91). The ancillary AgNOR technique provides a cheaper, more rapid and sensitive tool than routine lymphocyte counts in assessing the state of lymphoid proliferation in a variety of conditions in the dog.

Relationship between Canine Lymphocyte AgNOR Counts and Haematological Indices of Health

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A modified agyrophil technique was applied to peripheral blood smears to determine the mean AgNOR counts (MAC) of lymphocytes and ultimately assess the state...

Relationship between Canine Lymphocyte AgNOR Counts and Haematological Indices of Health

Published Online: Oct 16, 2018

Page range: 24 - 28

Received: Jun 06, 2018

Accepted: Aug 12, 2018

DOI: https://doi.org/10.2478/fv-2018-0023

Keywords
AgNOR, canine, haematology, lymphocyte

© 2018 Antia R. E. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Relationship between Canine Lymphocyte AgNOR Counts and Haematological Indices of Health

Published Online: Oct 16, 2018

Page range: 24 - 28

Received: Jun 06, 2018

Accepted: Aug 12, 2018

DOI: https://doi.org/10.2478/fv-2018-0023

KeywordsAgNOR, canine, haematology, lymphocyte

© 2018 Antia R. E. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
AgNOR, canine, haematology, lymphocyte