<abstract xmlns="http://www.w3.org/1999/xhtml">The interactions between the fungicide tebuconazole and human serum albumin were investigated using fluorescence and circular dichroism spectroscopies. The experimental results showed that the fluorescence quenching of the protein by the tebuconazole molecule was a result of the formation of a ligand-protein complex with a binding constant of 8.51×103 l.mol−1 and the number of binding sites in the macromolecule was close to 1. These findings demonstrated the fact that although the binding affinity of tebuconazole to the protein may be slight, it was very similar to other triazole fungicides. In addition, tebuconazole stabilized the α-helical secondary structure of the human serum albumin due to the increase of the α-content in the protein macromolecule.</abstract>

The interactions between the fungicide tebuconazole and human serum albumin were investigated using fluorescence and circular dichroism spectroscopies. The experimental results showed that the fluorescence quenching of the protein by the tebuconazole molecule was a result of the formation of a ligand-protein complex with a binding constant of 8.51×103 l.mol−1 and the number of binding sites in the macromolecule was close to 1. These findings demonstrated the fact that although the binding affinity of tebuconazole to the protein may be slight, it was very similar to other triazole fungicides. In addition, tebuconazole stabilized the α-helical secondary structure of the human serum albumin due to the increase of the α-content in the protein macromolecule.

Interaction of the Fungicide Tebuconazole with Human Serum Albumin: A Preliminary Study

University of Veterinary Medicine and Pharmacy

Institute of Biophysics and Informatics, First Faculty of Medicine

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The interactions between the fungicide tebuconazole and human serum albumin were investigated using fluorescence and circular dichroism spectroscopies. The...

Interaction of the Fungicide Tebuconazole with Human Serum Albumin: A Preliminary Study

Published Online: Jul 17, 2018

Page range: 85 - 91

Received: Mar 29, 2018

Accepted: May 18, 2018

DOI: https://doi.org/10.2478/fv-2018-0020

Keywords
association constant, fluorescence spectroscopy, human serum albumin, interaction, tebuconazole

© 2018 Staničová, J. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Interaction of the Fungicide Tebuconazole with Human Serum Albumin: A Preliminary Study

Published Online: Jul 17, 2018

Page range: 85 - 91

Received: Mar 29, 2018

Accepted: May 18, 2018

DOI: https://doi.org/10.2478/fv-2018-0020

Keywordsassociation constant, fluorescence spectroscopy, human serum albumin, interaction, tebuconazole

© 2018 Staničová, J. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
association constant, fluorescence spectroscopy, human serum albumin, interaction, tebuconazole