1. bookVolume 62 (2018): Issue 2 (June 2018)
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Protective Role of Agrimonia eupatoria L. in Heavy Metal Induced Nephrotoxicity

Published Online: 17 Jul 2018
Volume & Issue: Volume 62 (2018) - Issue 2 (June 2018)
Page range: 13 - 18
Received: 24 Oct 2017
Accepted: 06 Dec 2017
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

The aim of this study was to evaluate the potential protective role of Agrimonia eupatoria L. in heavy metal induced nephrotoxicity. Rabbit kidney epithelial cells (RK13) were used as the model cell line. They were exposed to three different heavy metal compounds: cadmium chloride dihydrate CdCl2.2H2O (15 and 20 mg.l−1), potassium dichromate K2Cr2O7 (1, 10 mg.l−1), and zinc sulfate heptahydrate ZnSO4.7H2O (50, 150 mg.l−1) simultaneously with agrimony (ethanolic extract, 100 mg.l−1). The cell response was recorded using the xCELLigence system or real-time cell analysis (RTCA) as a cell index (CI) and expressed as cell adherence (%) compared to control cells without treatment. The potential nephroprotective effects were recorded in cells treated with chromium (1 a 10 mg.l−1) and agrimony, where the cell adherence increased from 95.11 ± 11.25 % and 7.24 ± 0.33 % to 103.26 ± 1.23 % and 68.54 ± 4.89 % (P < 0.05) respectfully and also with a combination of agrimony and zinc (150 mg.l−1), where the adherence increased from 57.45 ± 1.98 % to 95.4 ± 6.95 %. During the cell exposure to cadmium in combination with agrimony, the protective effect was not recorded; the adherence of cells was even decreased (P < 0.05).

Keywords

1. Ad’hiah, A. L., Al-Bederi, O. N. H., Al-Sammarraie, K. W., 2013: Cytotoxic effects of Agrimonia eupatoria L. against cancer cell lives in vitro. J. Assoc. Arab. Univ. Basic Appl. Sci., 14, 87—92.10.1016/j.jaubas.2013.01.003Search in Google Scholar

2. Al-Snafi, A. E., 2015: The pharmacological and therapeutic importance of Agrimonia eupatoria. Asian Journal of Pharmaceutical Science and Technology, 5, 112—117.Search in Google Scholar

3. Barbier, O., Jacquillet, G., Tauc, M., Cougnon M., Poujeol, P., 2005: Effect of heavy metals on, and handling by, the kidney. Nephron Physiol., 99, 105—110.10.1159/00008398115722646Search in Google Scholar

4. Chan, S. M., Khoo, K. S., Sit, N. W., 2015: Interactions between plant extracts and cell viability indicators during cytotoxicity testing: implications for ethnopharmacological studies. Trop. J. Pharm. Res., 14, 1991—1998.10.4314/tjpr.v14i11.6Search in Google Scholar

5. Goyer, P. A., 2001:Casarett and Doull’s Toxicology: The Basic Science of Poisons. Toxic Effects of Metals. 6th edn., McGraw-Hill, USA, 811—867.Search in Google Scholar

6. He, Z. L., Yang, X. E., Stoffella, P. J., 2005: Trace elements in agroecosystems and impacts on the environment. J. Trace Elem. Med. Biol., 19, 125—140.10.1016/j.jtemb.2005.02.01016325528Search in Google Scholar

7. Johri, N., Jacquillet, G., Unwin, R., 2010: Heavy metal poisoning: the effects of cadmium on the kidney. Biometals, 23, 783—792.10.1007/s10534-010-9328-y20354761Search in Google Scholar

8. Marcinčáková, D., Falis, M., Legáth, J., Csank, T., Kšonžeková, P., Pistl, J., Milek, M., 2013: Monitoring of selected heavy metals cytotoxicity on xCELLigence real-time cell analyser. In Proceedings from the 33rd International Symposium: Industrial Toxicology, 2013, June, 19—21, Faculty of Chemical and Food Technology of the Slovak University of Technology, Bratislava, Slovakia, 165—168.Search in Google Scholar

9. Martinez-Serra, J., Gutierrez, A., Muñoz-Capó, S., Navarro-Palou, M., Ros, T., Amat, J. C., et al., 2014: xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies. Onco-Targets and Therapy, 7, 985—994.10.2147/OTT.S62887406116224959085Search in Google Scholar

10. Muruzović, M. Ž., Mladenović, K. G., Stefanović, O. D., Vasić, S. M., Čomić, L. R., 2016: Extracts of Agrimonia eupatoria L. as sources of biologically active compounds and evaluation of their antioxidant, antimicrobial, and antibiofilm activities. J. Food Drug Anal., 24, 539—547.10.1016/j.jfda.2016.02.00728911559Search in Google Scholar

11. Özdemir, A, Ark, M., 2013: xCELLigence Real Time Cell Analysis System: a new method for cell proliferation and cytotoxicity. Niche, 2, 15—17.10.5152/niche.2014.153Search in Google Scholar

12. Pan, T. H., Huang, B., Xing, J. Z., Zhang, W. P., Gabos, S., Chen, J., 2012: Recognition of chemical compounds in contaminated water using time-dependent multiple dose cellular responses. Anal. Chim. Acta, 724, 30—39.10.1016/j.aca.2012.03.00122483206Search in Google Scholar

13. Plum, L. M., Rink, L., Haase, H., 2010: The essential toxin: impact of zinc on human health. Int. J. Environ. Res. Public Health, 7, 1342—1365.10.3390/ijerph7041342287235820617034Search in Google Scholar

14. Reyes, J. L., Molina-Jijón, E., Rodríguez-Muñoz, R., Bautista-García, P., Debray-García, Y., Namorado, M. D. C., 2013: Tight junction proteins and oxidative stress in heavy metalsinduced nephrotoxicity. Biomed. Res. Int., http://dx.doi.org/10.1155/2013/730789.10.1155/2013/730789365462223710457Open DOISearch in Google Scholar

15. Tchounwou, P. B., Yedjou, C. G., Patlolla, A. K., Sutton, D. J., 2012: Heavy metal toxicity and the environment. Molecular, Clinical and Environmental Toxicology, Springer Basel, 133—164.10.1007/978-3-7643-8340-4_6414427022945569Search in Google Scholar

16. Xing, J. Z., Zhu, L., Jackson, J. A., Gabos S., Sun, X. Wang, X., Xu, X., 2005: Dynamic monitoring of cytotoxicity on microelectronic sensors. Chem. Res. Toxicol., 18, 154—161.10.1021/tx049721s15720119Search in Google Scholar

17. Xing, J. Z., Zhu, L., Gabos, S., Xie, L., 2006: Microelectronic cell sensor assay for detection of cyotoxicity and prediction of acute toxicity. Toxicology in Vitro, 20, 995—1004.10.1016/j.tiv.2005.12.00816481145Search in Google Scholar

18. Yoon, S. J., Koh, E. J., Kim, C. S., Zee, O. P., Kwak, J. H., Jeong, W. J., Kim, J. H., Lee, S. M., 2012:Agrimonia eupatoria protects against chronic ethanol induced liver injury in rats. Food Chem. Toxicol., 50, 2335—2341.10.1016/j.fct.2012.04.00522525864Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo