1. bookVolume 62 (2018): Issue 1 (March 2018)
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Immunohistochemical Study of the Goat Ductus deferens

Published Online: 11 Apr 2018
Volume & Issue: Volume 62 (2018) - Issue 1 (March 2018)
Page range: 11 - 17
Received: 18 Sep 2017
Accepted: 05 Dec 2017
Journal Details
License
Format
Journal
eISSN
2453-7837
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

Ductus deferens plays an important role in sperm transport and participates in the preservation of structure, maturation, and viability of sperm. In this study, we have immunohistochemically examined the ductus deferens in the goat. For immunohistochemical study the following monoclonal antibodies were used: cytokeratin 18, α-smooth muscle actin (α-SMA), vimentin and elastin. Morphologically, three distinct layers were identified in the goat ductus deferens — tunica mucosa, tunica muscularis and tunica adventitia. The epithelium of the mucosa was intensely stained with cytokeratin 18 (CK 18). The fibroblasts in the lamina propria and blood capillaries in the muscle layer showed positive reaction for vimentin. A positive reaction for α-SMA was observed in the smooth muscle cells of the tunica muscularis in the internal, middle and outer sublayers. An intense positive reaction for α-SMA was observed in the wall of the blood vessels. Elastic fibers in the form of a loose meshwork were present in all three layers. The high density of elastic fibers were found in the tunica adventitia.

Keywords

1. Abd-Elmaksoud, A. S., Ebada, S. M. Shoaib, M. B., 2012: Localisation of cytokeratin and smooth muscle actin in the accessory genital glands of camels (Camelus dromedarius) during rutting and non-rutting seasons. Bulg. J. Vet. Med., 15, 213—227.Search in Google Scholar

2. Achtstatter, T., Moll, R., Moore B., Franke, W. W., 1985: Cytokeratin polypeptide patterns of different epithelia of the human male urogenital tract: immunofluorescence and gel electrophoretic studies. J. Histoch. Cytochem., 33, 415—426.10.1177/33.5.2580881Search in Google Scholar

3. Alkafafy, M., 2009: Some immuno-histochemical studies on the epididymal duct in the donkey (Equus asinus). J. Vet. Anat., 2, 1—23.10.21608/jva.2009.45594Search in Google Scholar

4. Alkafafy, M., Attia, H., Rashed, R., Kandiel, M., 2010: Some comparative immunohistochemical studies on the ductus deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis). J. Vet. Anat., 3, 55—69.10.21608/jva.2010.42681Search in Google Scholar

5. Augsburger, H. R, 1997: Elastic fibres system of the female canine urethra. Histochemical identification of elastic, elaunin and oxytalan fibres. Anat. Histol. Embryol., 26, 297—302.940004810.1111/j.1439-0264.1997.tb00139.xSearch in Google Scholar

6. Flickinger, C. J., 1975: Fine structure of the rabbit epididymis and vas deferens after vasectomy. Biol. Reprod., 13, 50—60.10.1095/biolreprod13.1.50Search in Google Scholar

7. Francavilla, S., Moscardelli, S., Properzi, G., De Matteis, M. A., Scorza Barcellona, P. et al., 1987: Postnatal development of epididymis and ductus deferens in the rat. A correlation between the ultrastructure of the epithelium and tubule wall, and the fluorescence-microscopic distribution of actin, myosin, fibronectin, and basement membrane. Cell Tiss. Res., 249, 257—265.10.1007/BF00215508Search in Google Scholar

8. Kennedy, S. W., Heidger, P. M., 1979: Fine structural studies of the rat vas deferens. Anat. Rec., 194, 159—179.10.1002/ar.1091940111Search in Google Scholar

9. Khan, A. A., Zaidi, M. T., Faruqi, N. A., 2003:Ductus deferens — a comparative histology in mammals. J. Anat. Soc. India, 52, 163—165.Search in Google Scholar

10. Lohiya, N. K., Sharma, R. S., Ansari, A. S., Kumar, T. C., 1988: Structure of rete testis, vas efferens, epididymis and vas deferens of langur monkey (Presbytis entellus entellus Dufresne). Acta. Eur. Fertil., 19, 167—173.Search in Google Scholar

11. Manin, M., Lecher, P., Martinez, A., Tournadre, S., Jean, C., 1995: Exportation of mouse vas deferens protein, a protein without a signal peptide, from mouse vas deferens epithelium: A model of apocrine secretion. Biol. Reprod., 52, 50—62.10.1095/biolreprod52.1.50Open DOISearch in Google Scholar

12. Mcleod, D. G, Reynolds, D. G., Deamre, G. E., 1973: Some histological characteristics of the human vas deferens. Invest. Urology, 10, 338—341.Search in Google Scholar

13. Murakami, M., Nishida, T., Shiromoto M., Inokuchi, T., 1986: Scanning and transmission electron microscopy study of the ampullary region of the dog vas deferens with special reference to epithelial phagocytosis of spermatozoa. Anat. Anz., 162, 289—296.Search in Google Scholar

14. Murakumo, M., Ushiki, T., Abe, K., Matsumura, K., Shinno, Y., Koyanagi, T., 1995: Three-dimensional arrangement of collagen and elastin fibers in the human urinary bladder: a scanning electron microscopic study. J. Urol., 154, 251—256.10.1016/S0022-5347(01)67289-6Search in Google Scholar

15. Paniagua, R., Regadera, J., Nistal, M., Abauerrea, A., 1981: Histological, histochemical and ultrastructural variations along the length of the human vas deferens before and after puberty. Acta Anat., 111, 190—203.10.1159/0001454677064642Search in Google Scholar

16. Paniagua, R., Regadera, J., Nistal, M., Santamaria, L., 1983: Elastic fibres of the human ductus deferens. J. Anat., 137, 467—476.Search in Google Scholar

17. Powell, D. W., Mifflin, R. C., Valentich, J. D., Crowe, S. E., Saada, J. I., West, A. B., 1999: Myofibroblasts. I. Paracrine cells important in health and disease. Am. J. Physiol., 277, C 1—9.10.1152/ajpcell.1999.277.1.C110409103Search in Google Scholar

18. Regadera, J., Espana, G., Roias, M. A., Recio, J. A., Nistal, M., Suarez-Quian, C. A., 1997: Morphometric and immunocytochemical study of the fetal, infant, and adult human vas deferens. J. Androl., 18, 623—636.Search in Google Scholar

19. Ruan, Y. C., Wang, Z., Du, J. Y., Zuo, W. L., Guo, J. H., Zhang, J. et al., 2008: Regulation of smooth muscle contractility by the epithelium in rat vas deferens: role of ATP-induced release of PGE2. J. Physiol., 586, 4843—4857.10.1113/jphysiol.2008.154096261407018755753Search in Google Scholar

20. Setchell, B. P., Brooks, D. E., 2006: Anatomy, vasculature, innervation, and fluids of the male reproductive tract. Chapter 17, Vol. 1. In Knobil, E., Neill, J. D. (Eds.): The Physiology of Reproduction, 3rd edn., Raven press, New York, 770—825.10.1016/B978-012515400-0/50022-1Search in Google Scholar

21. Wiseman, O. J., Fowler, C. J., Landon, D. N., 2003: The role of the human bladder laminapropria myofibroblast. BJU Int., 91, 89—93.10.1046/j.1464-410X.2003.03802.xOpen DOISearch in Google Scholar

22. Williams, P. L., Banister, L. H., Berry, M. M., Collins, P., Dyson, M., Dussek, J. E., Ferguson, M. W. J., 1995: Gray’s Anatomy. In Reproductive System, 38th edn., Churchill Livingstone, New York, 1855—1856.Search in Google Scholar

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