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Fig. 1.

Antibacterial activity of textile fabric against Staphylococcus epidermidis ATCC 12228, Staphylococcus aureus ATCC 25923, Escherichiae coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Proteus mirabilis ATCC 12459 and Pseudomonas aeruginosa ATCC 15442: A – antibacterial activity of the samples; B – visualization of bacterial growth / non-growth under the sample (0 – sample 0; 1 – sample 1; 2 – sample 2)
Antibacterial activity of textile fabric against Staphylococcus epidermidis ATCC 12228, Staphylococcus aureus ATCC 25923, Escherichiae coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 13883, Proteus mirabilis ATCC 12459 and Pseudomonas aeruginosa ATCC 15442: A – antibacterial activity of the samples; B – visualization of bacterial growth / non-growth under the sample (0 – sample 0; 1 – sample 1; 2 – sample 2)

Fig. 2.

Antifungal activity of textile fabric against Candida albicans ATCC 10231: A – antifungal activity of the samples; B – visualization of candida growth / non-growth under the sample (0 – sample 0; 1 – sample 1; 2 – sample 2)
Antifungal activity of textile fabric against Candida albicans ATCC 10231: A – antifungal activity of the samples; B – visualization of candida growth / non-growth under the sample (0 – sample 0; 1 – sample 1; 2 – sample 2)

Antimicrobial activity test of the unwashed and washed textile fabric prepared – evaluation of bacterial and fungus growth

Sample identification Activity Reference cultures Growth Inhibition zone, mm Efficacy
Before antimicrobial treatment Antibacterial ATCC 12228 none 0 NE
ATCC 25922 none 0 NE
ATCC 25923 none 0 NE
ATCC 13883 none 0 NE
ATCC 27853 none 0 NE
ATCC 12459 none 0 NE
Antifungal ATCC 10231 none 0 NE
Sample 0 Antibacterial ATCC 12228 none 8.1 GE
ATCC 25922 none 2.1 GE
ATCC 25923 none 7.0 GE
ATCC 13883 none 3.0 GE
ATCC 27853 none 3.8 GE
ATCC 12459 none 1.3 GE
Antifungal ATCC 10231 none 1.8 GE
Sample 1 Antibacterial ATCC 12228 none 1.3 GE
ATCC 25922 none 0 GE
ATCC 25923 none 1.1 GE
ATCC 13883 none 0 GE
ATCC 27853 none 0 GE
ATCC 12459 none 0 GE
Antifungal ATCC 10231 none 0 GE
Sample 2 Antibacterial ATCC 12228 none 0 GE
ATCC 25922 none 0 GE
ATCC 25923 none 0 GE
ATCC 13883 none 0 GE
ATCC 27853 none 0 GE
ATCC 12459 none 0 GE
Antifungal ATCC 10231 none 0 GE

Assessment conditions of the antimicrobial and antifungal effect in the agar diffusion test

Mean value of inhibition zone (mm) Growth a) Description Assessment
> 11-00 NoneNoneNone Inhibition zone exceeding 1 mm, no growth b)Inhibition zone up to 1 mm, no growth b)No inhibition zone, no growth c) Good effect
0 Slight No inhibition zone, only some restricted colonies, growth nearly totally suppressed d) Limit of efficacy
00 ModerateHeavy No inhibition zone, compared to the control growth reduced to half e)No inhibition zone, compared to the control no growth reduction or only slightly reduced growth Insufficient effect

Evaluation of the electrostatic action of the unwashed and washed textile fabric prepared

Samples Resistance Remarks Recommendation
Without antimicrobial treatment 1.75×1012 Ω Higher than minimum compliance value according to the standard Not applicable for protective textiles
Antimicrobial treated and unwashed 1.17×109 Ω Good enough for this application as it is twice lower than the minimum compliance value Could be used for non-washable systems only
Antimicrobial treated and after 1st washing cycle 2.38×109 Ω On the limits of error with minimum possible Cannot be used for protective textiles.
Antimicrobial treated and after 2nd washing cycle --- Much higher than is required Cannot be used for protective textiles.