<abstract xmlns="http://www.w3.org/1999/xhtml"> The effects of different concentrations of copper ions (Cu2+ in the form of CuSO4 × 5H2O) on in vivo cucumber (Cucumis sativus L. ‘Edinstvo’) seedlings as well as on in vitro hypocotyl-derived callus were considered. Callus induction from hypocotyls was more prolific than from roots or cotyledons. Thus, callus obtained from hypocotyls of 7-day-old cucumber plants was cultured for 5 weeks on Murashige and Skoog medium containing 4 mg dm-3 2,4-D + 1 mg dm-3 BA supplemented with 0.01 mM, 0.1 mM or 1.0 mM of Cu2+. Biochemical indices related to oxidative stress were assessed. Cu2+ at 0.01 mM stimulated callus induction but 1.0 mM Cu2+ negatively affected callus formation and growth. LPO intensity was significantly lower than the control at all concentrations of Cu2+ but significantly higher than the control in plants exposed to 0.01 or 0.1 mM Cu2+. A similar trend was observed for the generation of the superoxide radical in both callus and plantlets. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APOX) activity increased in both callus and plantlets, but the level of increase in these antioxidant enzyme systems depended on the Cu2+ concentration. Cu2+ ions had a stronger (i.e., more negative) influence on oxidative stress in cucumber seedlings than on cucumber callus.</abstract>

The effects of different concentrations of copper ions (Cu2+ in the form of CuSO4 × 5H2O) on in vivo cucumber (Cucumis sativus L. ‘Edinstvo’) seedlings as well as on in vitro hypocotyl-derived callus were considered. Callus induction from hypocotyls was more prolific than from roots or cotyledons. Thus, callus obtained from hypocotyls of 7-day-old cucumber plants was cultured for 5 weeks on Murashige and Skoog medium containing 4 mg dm-3 2,4-D + 1 mg dm-3 BA supplemented with 0.01 mM, 0.1 mM or 1.0 mM of Cu2+. Biochemical indices related to oxidative stress were assessed. Cu2+ at 0.01 mM stimulated callus induction but 1.0 mM Cu2+ negatively affected callus formation and growth. LPO intensity was significantly lower than the control at all concentrations of Cu2+ but significantly higher than the control in plants exposed to 0.01 or 0.1 mM Cu2+. A similar trend was observed for the generation of the superoxide radical in both callus and plantlets. Superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APOX) activity increased in both callus and plantlets, but the level of increase in these antioxidant enzyme systems depended on the Cu2+ concentration. Cu2+ ions had a stronger (i.e., more negative) influence on oxidative stress in cucumber seedlings than on cucumber callus.

Prooxidative and antioxidative properties of cucumber (<italic>Cucumis sativus</italic> L.) callus <italic>in vitro</italic> and young <italic>in vivo</italic> plantlets in response to copper ions

Department of Botany and Plant Physiology

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prooxidative and antioxidative properties of cucumber ( L.) callus  and young  plantlets in response to copper ions

The effects of different concentrations of copper ions (Cu2+ in the form of CuSO4 × 5H2O) on in vivo cucumber (Cucumis sativus L. ‘Edinstvo’) seedlings as well...

Prooxidative and antioxidative properties of cucumber (Cucumis sativus L.) callus in vitro and young in vivo plantlets in response to copper ions

Published Online: Jan 22, 2014

Page range: 141 - 151

DOI: https://doi.org/10.2478/fhort-2013-0016

Keywords
ascorbate peroxidase, callus culture, catalase, lipid peroxidation, seedlings, superoxide anion, superoxide dismutase

© 2013 Alexander S. Lukatkin et al., published by Versita

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Prooxidative and antioxidative properties of cucumber (Cucumis sativus L.) callus in vitro and young in vivo plantlets in response to copper ions

Published Online: Jan 22, 2014

Page range: 141 - 151

DOI: https://doi.org/10.2478/fhort-2013-0016

Keywordsascorbate peroxidase, callus culture, catalase, lipid peroxidation, seedlings, superoxide anion, superoxide dismutase

© 2013 Alexander S. Lukatkin et al., published by Versita

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
ascorbate peroxidase, callus culture, catalase, lipid peroxidation, seedlings, superoxide anion, superoxide dismutase