The first biocorridors were established in the territory of the Czech Republic in the 1990s. One of them, planted on a former agricultural land, was the Radějov biocorridor. This paper deals with the growth and development of trees and shrubs on three permanent research plots in 1993 - 2012. Repeated inventories of trees as well as monitoring of their biometrical parameters were carried out in both tree and shrub layers. The number of trees decreases with the increasing level of stand canopy. Moreover, mean heights, diameters and crown projection areas of selected woody plants were compared. Under the given conditions, the growth of these woody plants can be positively evaluated.