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3D polyelectrolyte scaffolds to mimic exocrine glands: a step towards a prostate-on-chip platform

Nathalie Picollet-D’hahan

Nathalie Picollet-D’hahan

Université Grenoble Alpes, INSERM (UMR_S 1038), CEA, BIG-BGE, F-38054Grenoble, France
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Picollet-D’hahan, Nathalie
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Axel Tollance

Axel Tollance

Université Grenoble Alpes, INSERM (UMR_S 1038), CEA, BIG-BGE, F-38054Grenoble, France
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Tollance, Axel
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Cristina Belda Marin

Cristina Belda Marin

University Grenoble Alpes, SyNaBi, TIMCIMAG/CNRS/INSERM, UMR 5525, F-38000Grenoble, France
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Marin, Cristina Belda
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Lavinia Liguori

Lavinia Liguori

University Grenoble Alpes, SyNaBi, TIMCIMAG/CNRS/INSERM, UMR 5525, F-38000Grenoble, France
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Liguori, Lavinia
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Christophe Marquette

Christophe Marquette

Université Lyon 1, CNRS (UMR 5246), ICBMS, F-69622Villeurbanne, France
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Marquette, Christophe
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Odile Filhol-Cochet

Odile Filhol-Cochet

Université Grenoble Alpes, INSERM (UMR_S 1036), CNRS, BIG-BCI Biology of Cancer and Infection, F-38054Grenoble, France
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Filhol-Cochet, Odile
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Isabelle Vilgrain

Isabelle Vilgrain

Université Grenoble Alpes, INSERM (UMR_S 1036), CNRS, BIG-BCI Biology of Cancer and Infection, F-38054Grenoble, France
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Vilgrain, Isabelle
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Guillaume Laffitte

Guillaume Laffitte

Université Grenoble Alpes, CEA, LETI, DTBS, F-38054Grenoble, France
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Laffitte, Guillaume
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Florence Rivera

Florence Rivera

Université Grenoble Alpes, CEA, LETI, DTBS, F-38054Grenoble, France
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Rivera, Florence
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Jean-Pierre Alcaraz

Jean-Pierre Alcaraz

University Grenoble Alpes, SyNaBi, TIMCIMAG/CNRS/INSERM, UMR 5525, F-38000Grenoble, France
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Alcaraz, Jean-Pierre
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Jacques Thélu

Jacques Thélu

University Grenoble Alpes, SyNaBi, TIMCIMAG/CNRS/INSERM, UMR 5525, F-38000Grenoble, France
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Thélu, Jacques
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Olivier Nicoud

Olivier Nicoud

University Grenoble Alpes, SyNaBi, TIMCIMAG/CNRS/INSERM, UMR 5525, F-38000Grenoble, France
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Nicoud, Olivier
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Thibaud Moufle-Milot

Thibaud Moufle-Milot

University Grenoble Alpes, SyNaBi, TIMCIMAG/CNRS/INSERM, UMR 5525, F-38000Grenoble, France
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Moufle-Milot, Thibaud
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Maxime Legues

Maxime Legues

Université Grenoble Alpes, INSERM (UMR_S 1038), CEA, BIG-BGE, F-38054Grenoble, France
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Legues, Maxime
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Ali Bouamrani

Ali Bouamrani

Université Grenoble Alpes, CEA, LETI, CLINATEC, F-38000Grenoble, France
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Bouamrani, Ali
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Adrien Mombrun

Adrien Mombrun

Université Grenoble Alpes, CEA, LETI, CLINATEC, F-38000Grenoble, France
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Mombrun, Adrien
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Benoit Gilquin

Benoit Gilquin

Université Grenoble Alpes, CEA, LETI, CLINATEC, F-38000Grenoble, France
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Gilquin, Benoit
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Sophie Gerbaud

Sophie Gerbaud

Université Grenoble Alpes, INSERM (UMR_S 1038), CEA, BIG-BGE, F-38054Grenoble, France
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Gerbaud, Sophie
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Patricia Obeid

Patricia Obeid

Université Grenoble Alpes, INSERM (UMR_S 1038), CEA, BIG-BGE, F-38054Grenoble, France
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Obeid, Patricia
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Fréderique Kermarrec

Fréderique Kermarrec

Université Grenoble Alpes, INSERM (UMR_S 1038), CEA, BIG-BGE, F-38054Grenoble, France
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Kermarrec, Fréderique
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Xavier Gidrol

Xavier Gidrol

Université Grenoble Alpes, INSERM (UMR_S 1038), CEA, BIG-BGE, F-38054Grenoble, France
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Gidrol, Xavier
 and 
Donald K. Martin

Donald K. Martin

University Grenoble Alpes, SyNaBi, TIMCIMAG/CNRS/INSERM, UMR 5525, F-38000Grenoble, France
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Martin, Donald K.
  
Oct 27, 2018
3D polyelectrolyte scaffolds to mimic exocrine glands: a step towards a prostate-on-chip platform's Cover Image
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Article Category: Research Article
Published Online: Oct 27, 2018
Page range: 180 - 191
DOI: https://doi.org/10.2478/ebtj-2018-0048
Keywords
© 2018 Nathalie Picollet-D’hahan, Axel Tollance, Cristina Belda Marin, Lavinia Liguori, Christophe Marquette, Odile Filhol-Cochet, Isabelle Vilgrain, Guillaume Laffitte, Florence Rivera, Jean-Pierre Alcaraz, Jacques Thélu, Olivier Nicoud, Thibaud Moufle-Milot, Maxime Legues, Ali Bouamrani, Adrien Mombrun, Benoit Gilquin, Sophie Gerbaud, Patricia Obeid, Fréderique Kermarrec, Xavier Gidrol, Donald K. Martin, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

Mould design and fabrication. A. The Master mould was obtained by dropping a biocompatible resin on a rigid resin support. The PDMS final mould is composed by 200 hemi-spherical structures of ~ 100 μm on the base and ~ 50 um in heights. The pitch between the hemispherical wells is ~ 400 μm. B. Scanning electron microscopy (SEM) of the surface and the hemispheres. C. Confocal microscopy (orthogonal section) of the hemisphere coated with multi PE (15 layers alternating PSS-PAH) incubated with fluorescein. The fluorescein labels PE allowing the thin layer visualization. D. Confocal scanning laser microscopy of the hemispheres.
Mould design and fabrication. A. The Master mould was obtained by dropping a biocompatible resin on a rigid resin support. The PDMS final mould is composed by 200 hemi-spherical structures of ~ 100 μm on the base and ~ 50 um in heights. The pitch between the hemispherical wells is ~ 400 μm. B. Scanning electron microscopy (SEM) of the surface and the hemispheres. C. Confocal microscopy (orthogonal section) of the hemisphere coated with multi PE (15 layers alternating PSS-PAH) incubated with fluorescein. The fluorescein labels PE allowing the thin layer visualization. D. Confocal scanning laser microscopy of the hemispheres.

Figure 2

Conceptual view of a device integrating nanostructured free-standing supporting membrane. A. Schematic architecture of finger-like nanostructured scaffolds. The polyelectrolyte membrane (PEM, in red) creates acinar/ductal free standing and porous 3D structures that enclose the top of the microfluidic culture chamber to control the microenvironment for the cells. B. Close-up of the porous nanostructured membrane. Epithelial cells (EC, in green) are on the inside of the polyelectrolyte membrane. Cancerous cells are spiked from the outside of the polyelectrolyte membrane. Arrows indicate the secretions produced by EC. C. Schematic process of finger-like PE fabrication with sacrificial core (in purple).
Conceptual view of a device integrating nanostructured free-standing supporting membrane. A. Schematic architecture of finger-like nanostructured scaffolds. The polyelectrolyte membrane (PEM, in red) creates acinar/ductal free standing and porous 3D structures that enclose the top of the microfluidic culture chamber to control the microenvironment for the cells. B. Close-up of the porous nanostructured membrane. Epithelial cells (EC, in green) are on the inside of the polyelectrolyte membrane. Cancerous cells are spiked from the outside of the polyelectrolyte membrane. Arrows indicate the secretions produced by EC. C. Schematic process of finger-like PE fabrication with sacrificial core (in purple).

Figure 3

Schematic of alginate gelation process over 3D printed moulds. A. sacrificial alginate mould (c) is created over a 3D printed piece (a) to obtain a 3D shaped polyelectrolyte membrane (d). B. List of the 3D printed pieces. The assembly of these pieces allow the creation of PE pillar-like structures.
Schematic of alginate gelation process over 3D printed moulds. A. sacrificial alginate mould (c) is created over a 3D printed piece (a) to obtain a 3D shaped polyelectrolyte membrane (d). B. List of the 3D printed pieces. The assembly of these pieces allow the creation of PE pillar-like structures.

Figure 4

Hemisphere-shaped PE scaffolds. A. RWPE-1 cells cultured for 5 days on a PE coated mould. A deep color elaboration of the 3D acquisition shows the cellular distribution on the coated hemisphere (Red 0 μm- blue 50 μm). B. Calibration curve to determine the volume of secretion. C. Elispot detection of secretions from WPE1-int cells w/o DHT (a); WPE1-int w/DHT (b); RPTEC cells (negative control) (c) and 3 ng pure PSA (positive control) (d).
Hemisphere-shaped PE scaffolds. A. RWPE-1 cells cultured for 5 days on a PE coated mould. A deep color elaboration of the 3D acquisition shows the cellular distribution on the coated hemisphere (Red 0 μm- blue 50 μm). B. Calibration curve to determine the volume of secretion. C. Elispot detection of secretions from WPE1-int cells w/o DHT (a); WPE1-int w/DHT (b); RPTEC cells (negative control) (c) and 3 ng pure PSA (positive control) (d).

Figure 5

MALDI profiling statistical analysis of 3 cell lines supernatant. A. Principal Component Analysis of PC3, LNCaP and PNT2 spectra. B. Unsupervised hierarchical clustering of PC3, LNCaP and PNT2 spectra. Peaks used for 2D peak distribution are highlighted in orange. C. 2D peak distribution, plotting the intensities of peaks 2425 Da and 2482 Da across PC3, LNCaP and PNT2 spectra.
MALDI profiling statistical analysis of 3 cell lines supernatant. A. Principal Component Analysis of PC3, LNCaP and PNT2 spectra. B. Unsupervised hierarchical clustering of PC3, LNCaP and PNT2 spectra. Peaks used for 2D peak distribution are highlighted in orange. C. 2D peak distribution, plotting the intensities of peaks 2425 Da and 2482 Da across PC3, LNCaP and PNT2 spectra.

Figure 6

PNT2 cells co-cultured with LNCaP cells. A. Mixed population of non-cancerous (PNT2) and cancerous (LNCaP) prostatic cells. Confocal image of LNCaP labelled with CellTracker™_Green (here in red) on a PNT2 cells layer (here in green) cultured for 72h. Blue: Hoechst; Green Phalloidin; Red: LNCaP labelled with CellTracker™_Green. False colors are used for better visibility. Bar : 100 μm. B. 2D peak distribution of MALDI profiling analysis of PNT2 cells co-cultured with LNCaP cells.
PNT2 cells co-cultured with LNCaP cells. A. Mixed population of non-cancerous (PNT2) and cancerous (LNCaP) prostatic cells. Confocal image of LNCaP labelled with CellTracker™_Green (here in red) on a PNT2 cells layer (here in green) cultured for 72h. Blue: Hoechst; Green Phalloidin; Red: LNCaP labelled with CellTracker™_Green. False colors are used for better visibility. Bar : 100 μm. B. 2D peak distribution of MALDI profiling analysis of PNT2 cells co-cultured with LNCaP cells.
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