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Controlling beer filtration process through implementation of enzymatic and microbiological techniques


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Figure 1

Pilot scale apparatus and centrifuge used for beer filterability monitoring in experimental scale.
Pilot scale apparatus and centrifuge used for beer filterability monitoring in experimental scale.

Figure 2

Filtrability of different beers treated and not treated with enzymes.
Filtrability of different beers treated and not treated with enzymes.

Figure 3

Impact of malt quality and different enzymes used at brewhouse on beer filterability (Vmax).
Impact of malt quality and different enzymes used at brewhouse on beer filterability (Vmax).

Figure 4

Dynamic Viscosity (mPa s) in worts treated and non treated with enzymes (β-glucanase enzyme).
Dynamic Viscosity (mPa s) in worts treated and non treated with enzymes (β-glucanase enzyme).

Figure 5

Viscosity in worts and beer treated in different manner with enzymes.
Viscosity in worts and beer treated in different manner with enzymes.

Figure 6

Average Vmax and TSS for different treated beers.
Average Vmax and TSS for different treated beers.

The most critical parameters that impact on beer filtration and points where can be intervented in the process

Critical parameters that impact on beer filtrationIntervention in the process
Before Filtration
Number of yeast cells in the fermentation tankControlling yeast number in suspension through different
Number of yeast cells in the Bright Beer tankintervention.
Using of flocculants
Using of centrifugation process
Intervention to candle filter process (change kieselguhr dosage and nature)
Wort and Beer TurbidityControlling non-biological turbidity through enzymatic processes or different additives.
Wort and Beer ViscosityControlling non-biological turbidity through enzymatic processes
During Filtration Process
Differential pressure on the filterIntervention to candle filter process (change kieselguhr dosage and nature)
Intervention to beer nature regarding, microbiological load, and non microbiological turbidity (protein, carbohydrates and polyphenols)
Turbidity at filter outletControlling number of yeast and other spoilage microorganisms Recycling
Use of enzymes and different stabilisants Reorganizations of filter-aid dosage
Microbiologic quality monitoringIntervention to hygiene measure Reorganizations of filter-aid dosage
After Filtration
TurbidityUse of different stabilisants to control stability
Microbiologic quality monitoringIntervention to hygiene measure Reorganizations of filter-aid dosage

Nature of Particles in suspension in beer

Nature of particles in suspensionCompositionSourceParticle size in μm
MicroorganismsBrewing yeast (Saccharomyces carlsbengensis) + wild yeast (Saccharomyces sp.)Fermentation process + contamination5 – 10 μm
Bacteria (Lactobacillus sp., Pediococcus sp.)Contamination0.2 – 2 μm
TurbidityCarbohydrates turbidity (stable turbidity)BrewhouseUp to 3 μm
Proteins and PoliphenolsBrewhouse

Evaluation of most critical stability parameters of beer from fermentation to the Bright Beer Tank (These processes are in chain and experiments were carried out in industrial scale)

In the end of FermentationAfter centrifugationAfter filter aid tratmentClarification processAfter kiselguhr filtration
Yeast cell cell/number ml5-30x1061-10x1051-8x1051-5x1050-10
Turbidity EBC2231852101600.5
Total N mg/l9085554340
Poliphenols mg/l2315650.8

Experimental measurements for cell number using free yeast and immobilized yeast in alginate beads (Using industrial scale wort)

Number of yeast cells in sunspension (cell/ml)Free yeast fermentationImmobilized yeast fermentation
Inoculation cell number10 x 106 - 20 x 10610 x 106 - 20 x 106
Yeast remain cell in number suspension in the end of fermentation25 -100 x 1061-70 x 106
vmax10 - 35260 - 330
TSS (mg/l)21050 - 90
eISSN:
2564-615X
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Genetics, Biotechnology, Bioinformatics, other