Cite

Figure 1.

Schematic diagram comparing air flow path for the closed-end HTP-A (a: the test product) and an open-end HTB-B (b: see Ref. 8, 9 )
Schematic diagram comparing air flow path for the closed-end HTP-A (a: the test product) and an open-end HTB-B (b: see Ref. 8, 9 )

Figure 2.

Cell viability in NRU assay of HTP-A and HTP-B aerosol, and 3R4F smoke. Cell viability of CHO cells was determined by NRU assay following 24 h exposures of DMSO (a) or HTP-A (b), HTP-B (c) and 3R4F (d). The data represent the mean ± SD (n = 3) (###P < 0.01).
Cell viability in NRU assay of HTP-A and HTP-B aerosol, and 3R4F smoke. Cell viability of CHO cells was determined by NRU assay following 24 h exposures of DMSO (a) or HTP-A (b), HTP-B (c) and 3R4F (d). The data represent the mean ± SD (n = 3) (###P < 0.01).

Salmonella typhimurium strains characteristics, source and positive controls.

Strains Mutation Antibiotic resistance Positive controls
Without S9 (μg/plate) With S9 (μg/plate)
TA98 His D3052 Ampicillin 2-Nitrofluorene (4 μg/plate) 2-Amlinofluorene (10 μg/plate)
TA100 His G46 Ampicillin 4-Nitroquinoline N-oxide (0.5 μg/plate) 2-Amlinofluorene (10 μg/plate)

In vitro mammalian cell micronucleus test.

Positive controls Ultimate density
Cyclophosphamide A (short-term + S9) 0.2 μg/mL
Mitomycin C (short-term - S9) 1.0 μg/mL
Mitomycin C (long-term - S9) 0.5 μg/mL

Revertant colonies of Salmonella typhimurium obtained following exposure to different doses of TPM from HTP-A and HTP-B.

Dose (μg/plate) TA98 TA100
HTP-A HTP-B HTP-A HTP-B
−S9
NC 38.0 ± 2.4 38.0 ± 2.4 115.3 ± 3.8 115.3 ± 3.8
0 25.7 ± 4.0 25.7 ± 4.0 121.7 ± 6.0 121.7 ± 6.0
50 24.0 ± 3.7 30.3 ± 4.0 124.0 ± 4.1 108.0 ± 9.9
500 35.0 ± 3.7 28.7 ± 4.1 120.3 ± 8.7 105.0 ± 8.3
1000 30.7 ± 2.4 33.3 ± 5.7 114.3 ± 6.2 129.3 ± 5.3
2000 30.7 ± 3.8 32.7 ± 4.5 120.7 ± 7.9 125.3 ± 3.1
PC 899.0 ± 24.0 899.0 ± 24.0 2719.7 ± 55.5 2719.7 ± 55.5
         
+S9
NC 60.0 ± 5.9 60.0 ± 5.9 156.3 ± 2.9 156.3 ± 2.9
0 51.0 ± 5.1 51.0 ± 5.1 107.0 ± 6.4 107.0 ± 6.4
50 48.3 ± 7.6 48.3 ± 3.1 116.0 ± 19.0 100.0 ± 4.3
500 48.7 ± 7.4 56.0 ± 4.3 112.0 ± 10.7 100.7 ± 5.4
1000 49.0 ± 7.1 40.3 ± 5.2 113.7 ± 12.4 100.7 ± 9.2
2000 51.0 ± 2.8 41.7 ± 7.0 94.0 ± 16.8 105.3 ± 1.2
PC 2437.3 ± 106.0 2437.3 ± 106.0 781.7 ± 95.7 781.7 ± 95.7

Selected toxicant yields from two HTPs and 3R4F.

Analyte Unit HTP-A HTP-B 3R4F
Nicotine mg/stick 0.47 ± 0.01 0.98 1.05
Total particulate matter mg/stick 29.06 ± 1.36 30.15 24.23
  mg/mg nicotine 61.83 ± 2.90 30.77 23.08
Carbon monoxide mg/stick 0.19 ± 0.01 0.25 24.69
  mg/mg nicotine 0.40 ± 0.02 0.26 23.52
Formaldehyde μg/stick 1.08 ± 0.18 4.46 ± 0.56 26.60 ± 2.14
  μg/mg nicotine 2.30 ± 0.38 4.55 ± 0.57 25.33 ± 2.04
Acetaldehyde μg/stick 123.5 ± 19.53 172.42 ± 6.10 722.47 ± 36.11
  μg/mg nicotine 262.77 ± 41.55 175.94 ± 6.22 688.07 ± 34.39
Acrolein μg/stick 4.78 ± 0.91 10.90 ± 0.18 71.92 ± 2.84
  μg/mg nicotine 1.02 ± 1.94 11.12 ± 0.18 68.50 ± 2.70
1,3-Butadiene μg/stick NQ NQ 8.13 ± 0.17
  μg/mg nicotine NQ NQ 7.74 ± 0.16
Benzene μg/stick 0.17 ± 0.00 0.36 ± 0.05 49.95 ± 1.54
  μg/mg nicotine 0.36 ± 0.00 0.37± 0.05 47.57 ± 1.47
Benzo(a)pyrene ng/stick NQ NQ 24.00 ± 0.64
  ng/mg nicotine NQ NQ 22.86 ± 0.61
NNK ng/stick 6.61 ± 0.86 5.32 ± 0.89 281.00 ± 16
  ng/mg nicotine 14.06 ± 0.83 5.43 ± 0.91 267.62 ± 15
NNN ng/stick 1.79 ± 0.26 8.37 ± 0.55 163.43 ± 11.19
  ng/mg nicotine 3.81 ± 0.55 8.54 ± 0.56 155.65 ± 10.66

Revertant colonies of Salmonella typhimurium obtained following exposure to different doses of TPM from 3R4F.

Dose (μg/plate) TA98 TA100
−S9 +S9 −S9 +S9
NC 38.0 ± 2.4 60.0 ± 5.9 115.3 ± 3.8 156.3 ± 2.9
0 25.7 ± 4.0 51.0 ± 5.1 121.7 ± 6.0 107.0 ± 6.4
50 38.3 ± 8.2 87.7 ± 5.2 125.7 ± 8.5 105.0 ± 3.7
100 28.3 ± 7.5 124.3 ± 5.4* 127.3 ± 7.6 115.3 ± 7.5
250 32.7 ± 6.1 237.7 ± 11.8* 143.3 ± 8.3 133.0 ± 4.1
500 34.7 ± 2.1 233.7 ± 33.0* 137.7 ± 11.1 171.7 ± 13.0
PC 899.0 ± 24.0 2437.3 ± 106.0 2719.7 ± 55.5 781.7 ± 95.7

Analytical procedures used for HTP aerosol and cigarette smoke chemical analyses.

Analyte Brief description Reference
Nicotine By gas chromatography with flame ionization detection ISO 10315 (12)
CO By nondispersive infrared photometry ISO 8454 (13)
Polycyclic aromatic hydrocarbons Extracted by hexane and analyzed by GC-MS CRM 91 (14)
N-nitrosamines Extracted by acetic acid and analyzed by HPLC-MS/MS CRM 75 (15)
Volatile organic compounds By GC-MS using simultaneous trapping of adsorbent cartridges in a Cambridge filter without cryogenic impinger WHO SOP 09 (16)
Carbonyl compounds By HPLC, using simultaneous trapping of adsorbent cartridges in a Cambridge filter without cryogenic impinge WHO SOP 08 (17)

Cell genotoxicity of different TPM fractions of HTP-A and HTP-B, and 3R4F.

Sample Concentration (μg/mL) MN frequencies (%)
3h + 21h -S9 3h + 21h +S9 24h + 0h -S9
Vehicle 11 1.49 ± 0.23 1.52 ± 0.54 1.08 ± 0.08
HTP-A
75 1.37 ± 0.33 1.28 ± 0.04 1.29 ± 0.08
150 1.08 ± 0.37 1.20 ± 0.07 1.19 ± 0.04
300 1.05 ± 0.07 1.21 ± 0.01 1.40 ± 0.08
600 1.15 ± 0.17 1.10 ± 0.10 1.30 ± 0.01
HTP-B
75 1.11 ± 0.02 1.20 ± 0.14 1.26 ± 0.16
150 1.33 ± 0.22 1.07 ± 0.18 1.28 ± 0.11
300 1.36 ± 0.30 1.28 ± 0.01 1.33 ± 0.13
600 1.69 ± 0.30 1.29 ± 0.21 1.32 ± 0.11
3R4F
14.3 2.03 ± 0.21 2.16 ± 0.92 2.53 ± 0.05
28.5 3.21 ± 1.02 2.46 ± 0.98 2.85 ± 0.20
57.0 3.93 ± 1.34 2.31 ± 1.29 2.65 ± 0.05
114.0 4.49 ± 1.58 2.67 ± 1.49 3.03 ± 0.30
PC - 5.68 ± 0.29 5.08 ± 0.55 6.09 ± 0.37
NC - 1.40 ± 0.30 1.32 ± 0.30 1.20 ± 0.11

Overview of test product specifications. The heating device of HTP-A is branded as Mr. Yeah and the tobacco sticks carry the brand FARSTAR. HTP-B consists of an IQOS™ branded heating device and TEREA branded tobacco sticks.

Parameter HTP-A HTP-B Combustible cigarette
Commercial product (name) Mr. Yeah IQOS™ 3R4F
Puffing air flow design Closed-end Open-end Open
Heater design External heating Central heating Combustion
Maximum heating temp. (°C) 260 350 /
Puff n° under HCI regime 8 12 8
Tobacco stick-length (mm) 45 45 84
Tobacco stick-weight (mg) 721.70 664.30 1035.00
Tobacco stick-diameter (mm) 7.36 7.18 7.98
eISSN:
2719-9509
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
General Interest, Life Sciences, other, Physics