1. bookVolume 35 (2022): Issue 2 (August 2022)
Journal Details
License
Format
Journal
eISSN
2300-6676
First Published
30 May 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates

Published Online: 02 Sep 2022
Volume & Issue: Volume 35 (2022) - Issue 2 (August 2022)
Page range: 58 - 61
Received: 19 Jan 2022
Accepted: 24 Jun 2022
Journal Details
License
Format
Journal
eISSN
2300-6676
First Published
30 May 2014
Publication timeframe
4 times per year
Languages
English
Abstract

Investigations into separation selectivity of High-Performance Thin-layer chromatography (HPTLC) of dansyl (DNS) derivatives of amino acids in silica gel (Silica gel 60 F254s plates) and silanized silica gel (RP-18 W plates) systems are presented. The results have been obtained for mobile phases containing different concentrations of acetonitrile (ACN) in formic acid (FA) water solution (final concentration of FA in the mobile phase was equal to 265 mmol/dm3). The data obtained show differences in separation selectivity of the solutes between employment of HPTLC silica gel and RP-18 W systems.

Keywords

1. Roda JM, Pascual JM, Carceller F, González-Llanos F, Pérez-Higueras A, Solivera J, et al. Nonhistological diagnosis of human cerebral tumors by 1H magnetic resonance spectroscopy and amino acid analysis. Clin Cancer Res. 2000;6:3983-93. Search in Google Scholar

2. Pisters PWT, Pearlstone DB. Protein and amino acid metabolism in cancer cachexia: investigative techniques and therapeutic interventions. Crit Rev Clin Lab Sci. 1993;3:223-72. Search in Google Scholar

3. Herbet M, Natorska Chomicka D, Korga A, Ostrowska M, Izdebska M, Gawrońska Grzywacz M, et al. Altered expression of genes involved in brain energy metabolism as adaptive responses in rats exposed to chronic variable stress; changes in cortical level of glucogenic and neuroactive amino acids. Mol Med Rep. 2019;19:2386-96.10.3892/mmr.2019.9865 Search in Google Scholar

4. Tajiri K, Shimizu Y. Branched-chain amino acids in liver diseases. World J Gastroenterol. 2013;43:7620-9.10.3748/wjg.v19.i43.7620 Search in Google Scholar

5. Weiss R, Kim K. Metabolomics in the study of kidney diseases. Nat Rev Nephrol. 2012;8:22-33.10.1038/nrneph.2011.152 Search in Google Scholar

6. Shockcor JP, Holmes E. Metabonomic applications in toxicity screening and disease diagnosis. Curr Top Med Chem. 2002;2:35-51.10.2174/1568026023394498 Search in Google Scholar

7. Kupec J, Turina S. The determination of some amino acids by conventional and hot plate chromatography. Chromatographia. 1977;10:157-9.10.1007/BF02297870 Search in Google Scholar

8. Wollenweber P. Separation of amino acids by thin-layer chromatography on cellulose. J Chromatogr. 1962;9:369-71.10.1016/S0021-9673(00)80801-8 Search in Google Scholar

9. Haworth C, Heathcote JG. The direct determination of amino acids on thin-layer chromatograms by densitometry. Biochem J. 1969; 114:667-8.10.1042/bj114066711849445820651 Search in Google Scholar

10. Bhushan R, Ali I. TLC resolution of amino Acids in a new solvent and effect of alkaline earth metals. J Liq Chromatogr. 1987;10:3647-52.10.1080/01483918708077820 Search in Google Scholar

11. Sleckman BP, Sherma J. A Comparison of amino acid separations on silica gel, cellulose, and ion exchange thin layers. J Liq Chromatogr. 1982;5:1051-68.10.1080/01483918208067568 Search in Google Scholar

12. Vasta JD, Cicchi M, Sherma J, Fried B. Evaluation of thin-layer chromatography systems for analysis of amino acids in complex mixtures. Acta Chromatogr. 2009;21:29-38.10.1556/AChrom.21.2009.1.3 Search in Google Scholar

13. Sherma J, Sleckman BP, Armstrong DW. Chromatography of amino acids on reversed phase thin layer plates. J Liq Chromatogr. 1983;6:95-108.10.1080/01483918308066873 Search in Google Scholar

14. Baranowska I, Kozłowska M. TLC separation and derivative spectrophotometry of some amino acids. Talanta. 1995;42:1553-7.10.1016/0039-9140(95)01569-W Search in Google Scholar

15. Polak B, Hałka A, Dzido TH. Pressurized planar electrochromato-graphic separation of the enantiomers of tryptophan and valine. J Planar Chromatogr. 2008;21:33-7.10.1556/JPC.21.2008.1.5 Search in Google Scholar

16. Polak B, Wojtanowski KK, Ślązak P, Dzido TH. Separation of some aromatic amino acid enantiomers with pressurized planar electrochromatography and TLC. Chromatographia. 2011;73:339-45.10.1007/s10337-010-1888-z Search in Google Scholar

17. Polak B, Balasa K, Dzido TH. Separation of amino acid 2,4-Dinitrophenyl-5-L-valine amide diastereomeric derivatives with high-performance planar chromatography and pressurized planar electrochromatography. J Planar Chromatogr. 2013;28:180-9.10.1556/JPC.26.2013.2.13 Search in Google Scholar

18. Polak B, Maruszak A, Płocharz PW. High-performance thin-layer chromatography and pressurized planar electrochromatography of some diastereomeric amino acid derivatives in reversed -phase system with carboxylic acid mobile phase buffers. J Planar Chromatogr. 2016;29:22-9.10.1556/1006.2016.29.1.3 Search in Google Scholar

19. Polak B, Garbacz P. β- cyclodextrin as the mobile phase component for separation of some DNS-amino acid enantiomers with HPTLC and PPEC. Curr Anal Chem. 2015;11:68-77.10.2174/1573411010666141127211110 Search in Google Scholar

20. Chomicki A, Kloc K, Dzido TH. Two-dimensional separation of some amino acids by HPTLC and pressurized planar electrochromatography. J Planar Chromatogr. 2011;24:16-9.10.1556/JPC.24.2011.1.1 Search in Google Scholar

21. Chomicki A, Dzido TH, Materna-Witkowska E, Klimek-Turek A. Thin-layer chromatography and pressurized planar electrochromatography of amino acids in systems with silica gel and water mobile phase. J Planar Chromatogr. 2016;29:30-7.10.1556/1006.2016.29.1.4 Search in Google Scholar

22. Fager RS, Kutina CB. Resolution of dansyl leucine and dansyl isoleucine. J Chromatogr. 1973;76:268-9.10.1016/S0021-9673(01)97808-2 Search in Google Scholar

23. Bhushan R, Reddy GP. Some solvent systems for the resolution of dansyl amino acids by silicagel thin layer chromatography. J Li Chromatogr. 1988;11:3163-70.10.1080/01483918808076786 Search in Google Scholar

24. Wood KR, Wang KT. Separation of dansyl-amino acids by polyamide layer chromatography. Biochem Biophys Acta. 1967;133:369-70. Search in Google Scholar

25. Crowshaw K, Jessup SJ, Ramwell PW. Thin-layer chromatography of 1-dimethylaminonaphthalene-5-sulphonyl derivatives of amino acids present in superfusates of cat cerebral cortex. Biochem J. 1967;103:79-85.10.1042/bj1030079 Search in Google Scholar

26. Biou D, Queyrel N, Visseaux MN, Collignon I, Pays M. Separation and identification of dansylated human serum and urinary amino acids by two-dimensional thin-layer chromatography : Application to aminoacidopathies. J Chromatogr. 1981;226:477-83.10.1016/S0378-4347(00)86084-3 Search in Google Scholar

27. Wesenberg JC, Jacqueline EJ. Screening for amino acid disorders by thin-layer chromatography of the dansyl amino acids. Microchim Acta. 1980;74:1-7.10.1007/BF01196480 Search in Google Scholar

28. Macek K, Deyl Z, Smrz M. Two-dimensional thin-layer chromatography of Dns-amino acids on reversed-phase silica gel. J Chromatogr A. 1980;193:521-426.10.1016/S0021-9673(00)87742-0 Search in Google Scholar

29. Lepri L, Coas V, Desideri PG. et al. Reversed phase planar chromatography of dansyl DL amino acids with bovine serum albumin in the mobile phase. Chromatographia. 1993;36:297-301.10.1007/BF02263881 Search in Google Scholar

30. Sinibaldi M, Messina A, Girelli AM. Separation of dansylamino acid enantiomers by thin-layer chromatography. Analyst. 1988;113:1245-7.10.1039/an9881301245 Search in Google Scholar

31. Weinstein S. Resolution of optical isomers by thin layer chromatography. Tetrahedron Lett. 1984;25:985-6.10.1016/S0040-4039(01)80080-0 Search in Google Scholar

32. LeFevre JW, Dodsworth DW. Complete analysis of a biologically active tetrapeptide: A project utilizing thin-layer chromatography and mass spectrometry. J Chem Educ. 2000;77:503-4.10.1021/ed077p503 Search in Google Scholar

33. LeFevre JW, Gublo EJ, Botting C, Wall R, Nigro A, Pham MLT, Ganci G. Qualitative reversed-phase thin-layer chromatographic analysisof the stereochemistry of D- and L-alpha-amino acids in small peptides. J Planar Chromatogr. 2000;13:160-5. Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo