Thin-layer chromatography of DNS amino acids derivatives in systems with silica gel and silanized silica gel plates
Adam Chomicki
Department of Physical Chemistry, Medical University of LublinPoland
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and Tadeusz H. Dzido
Department of Physical Chemistry, Medical University of LublinPoland
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Sep 02, 2022
About this article
Published Online: Sep 02, 2022
Page range: 58 - 61
Received: Jan 19, 2022
Accepted: Jun 24, 2022
DOI: https://doi.org/10.2478/cipms-2022-0011
Keywords
© 2022 Adam Chomicki et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Investigations into separation selectivity of High-Performance Thin-layer chromatography (HPTLC) of dansyl (DNS) derivatives of amino acids in silica gel (Silica gel 60 F254s plates) and silanized silica gel (RP-18 W plates) systems are presented. The results have been obtained for mobile phases containing different concentrations of acetonitrile (ACN) in formic acid (FA) water solution (final concentration of FA in the mobile phase was equal to 265 mmol/dm3). The data obtained show differences in separation selectivity of the solutes between employment of HPTLC silica gel and RP-18 W systems.