1. bookVolume 58 (2014): Issue 2 (June 2014)
Journal Details
License
Format
Journal
eISSN
2450-8608
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Hepatic stellate cells in the liver of dogs with steroid-induced hepatopathy

Published Online: 17 Jun 2014
Volume & Issue: Volume 58 (2014) - Issue 2 (June 2014)
Page range: 273 - 276
Received: 20 Jul 2013
Accepted: 05 Jun 2014
Journal Details
License
Format
Journal
eISSN
2450-8608
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

Morphological lesions in hepatic stellate cells caused by the immunosuppressive doses of dexamethasone were investigated in dogs. The archival samples of liver collected during a surgical biopsy were examined. The samples were fixed in 10% buffered formalin or Carnoy’s solution and then stained with routine histochemical methods. The lesions were also investigated under electron microscope. It was demonstrated that the number of stellate cells significantly increased (P = 0.0277), yet the size of cytoplasmic lipid droplets significantly decreased (P = 0.0001). Even though steroid-induced hepatopathy is considered to be a reversible pathology, and the lesions in hepatocytes under the influence of dexamethasone occur in a short period, it was found that hepatic stellate cells proliferated and underwent activation. This resulted in collagen accumulation in the hepatic sinuses. The functional and morphological disturbances in the canine liver in the course of steroid-induced hepatopathy are initially subclinical, but the changes in the structure and function of hepatic stellate cells may become a cause of lesions in the wall of hepatic sinusoidal vessels, which may induce additional functional pathologies unrelated to the damage to hepatocytes.

Keywords

1. Atzori L., Poli G., Perra A.: Hepatic stellate cells: a star cell in the liver. Int J Biochem Cell Biol 2009, 41, 1639-1642.10.1016/j.biocel.2009.03.00119433304 Search in Google Scholar

2. Bancroft J.D., Cook H.C.: Manual of histological techniques and their diagnostic application. Churchill Livingstone, Edinburgh, London, Madrid, Melbourne, New York, Tokyo, 2000. Search in Google Scholar

3. Budny T., Palmers D., Stratman U., Minin E., Herbst H., Spiegel H.U.: Morphologic features in the regenerating liver - a comparative intravital, light microscopical and ultrastructural analysis with focus on hepatic stellate cells. Virchows Arch 2007, 451, 781-791.10.1007/s00428-007-0472-x17674035 Search in Google Scholar

4. Carpino G., Franchito A., Morini S., Corradini S.G., Merli M., Gaudio E.: Activated hepatic stellate cells in liver cirrhosis. A morphologic and morphometrical study. Ital J Anat Embryol 2004, 109, 225-238. Search in Google Scholar

5. Flisiak R.: Role of Ito cells in the liver function. Pol J Pathol 1997, 48, 139-145. Search in Google Scholar

6. Friedman S.: Hepatic stellate cells: protean, multifunctional and enigmatic cells of the liver. Physiol Rev 2008, 88, 125-172.10.1152/physrev.00013.2007288853118195085 Search in Google Scholar

7. Hellerbrand C.: Hepatic stellate cells - the pericites in the liver. Pflugers Arch 2013, 465, 775-778.10.1007/s00424-012-1209-523292551 Search in Google Scholar

8. Kmieć Z.: The role of hepatic stellate cells in the regulation of the liver functions. II. Cooperation with other cell types in the development of liver fibrosis. Advances in Cell Biol 2002, 30, 61-74. Search in Google Scholar

9. Maxie M.: Jubb Kennedy and Palmer’s Pathology of Domestic Animals, Vol. 2, Chapter 2, Liver and biliary system, Elsevier Saunders, Philadephia, 2007, pp. 301-304. Search in Google Scholar

10. Ostrowski K.: Histologia, PZWL, Warszawa, 1995. Search in Google Scholar

11. Plewka K., Szuster-Ciesielska A., Kandefer-Szerszeń M.: Role of stellate cells in alcoholic liver fibrosis. Postępy Hig Med Dośw 2009, 63, 303-317. Search in Google Scholar

12. Rothuizen J., Bunch S., Charles J., Cullen J., Desmet V., Szatmari V., Twedt D., van den Ingh T.S.G.A.M., Van Winkle T., Washabau R.: WSAVA standards for clinical and histological diagnosis of canine and feline liver diseases, WSAVA Liver Standardization Group, Saunders Elsevier, Spain, 2006, pp. 41-59. Search in Google Scholar

13. Tomanovic N.R., Boricic I.V., Brasanac D.C., Stojsic Z.M., Delic D.S., Brmbolic B.J.: Activated liver stellate cells in chronic viral C hepatitis: histopathological and immunohistochemical study. J Gastrointestin Liver Dis 2009, 18, 163-167. Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo