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Comparison of FGF-8, FGF-10, FGF-Receptor 2, Androgen Receptor, Estrogen Receptor-A and SS in Healthy and Hypospadiac Children

N Emaratpardaz

N Emaratpardaz

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Emaratpardaz, N
, 
Z Turkyilmaz

Z Turkyilmaz

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Turkyilmaz, Z
, 
R Karabulut

R Karabulut

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Karabulut, R
, 
D Dayanir

D Dayanir

Gazi University Faculty of Medicine, Departments of Histology and EmbryologyAnkara, Turkey
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Dayanir, D
, 
C Kaya

C Kaya

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Kaya, C
, 
AAE Sert

AAE Sert

Gazi University Faculty of Medicine, Departments of Histology and EmbryologyAnkara, Turkey
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Sert, AAE
, 
G Arkan

G Arkan

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Arkan, G
, 
FA Ucaner

FA Ucaner

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Ucaner, FA
, 
A Kapisiz

A Kapisiz

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Kapisiz, A
, 
S Eryilmaz

S Eryilmaz

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Eryilmaz, S
, 
A Atan

A Atan

Gazi University Faculty of Medicine, Departments of UrologyAnkara, Turkey
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Atan, A
 and 
K Sonmez

K Sonmez

Gazi University Faculty of Medicine, Departments of Pediatric SurgeryAnkara, Turkey
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Sonmez, K
  
Sep 06, 2024
Comparison of FGF-8, FGF-10, FGF-Receptor 2, Androgen Receptor, Estrogen Receptor-A and SS in Healthy and Hypospadiac Children's Cover Image
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Article Category: Original Article
Published Online: Sep 06, 2024
Page range: 21 - 29
DOI: https://doi.org/10.2478/bjmg-2024-0002
Keywords
© 2024 N Emaratpardaz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

AR uptake was observed in basal keratinocytes in control group. AR involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
AR uptake was observed in basal keratinocytes in control group. AR involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).

Figure 2.

ER-α uptake was observed in basal keratinocytes in control group. ER-α involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
ER-α uptake was observed in basal keratinocytes in control group. ER-α involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).

Figure 3.

ER-β uptake was observed in basal keratinocytes in control group. ER-β involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).
ER-β uptake was observed in basal keratinocytes in control group. ER-β involvement was limited to the lower 2/3 of the epidermis in the hypospadias group (IHC Staining, 200 X).

Figure 4.

In the control group, FGFR-2 uptake was observed in more than 50% of Keratinocytes (Epidermis Pattern 3) and clustered positive cells in more than 50% of all visible dermal cells (Dermis Pattern C). Limited to basal involvement in hypospadias group (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
In the control group, FGFR-2 uptake was observed in more than 50% of Keratinocytes (Epidermis Pattern 3) and clustered positive cells in more than 50% of all visible dermal cells (Dermis Pattern C). Limited to basal involvement in hypospadias group (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).

Figure 5.

In the control Group, more than 50% of keratinocytes were involved (Epidermis Pattern 3) and less than 50% of dermal cells are involved (Dermis Pattern B) for FGF-8. In the hypospadias group, it was limited to basal involvement (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
In the control Group, more than 50% of keratinocytes were involved (Epidermis Pattern 3) and less than 50% of dermal cells are involved (Dermis Pattern B) for FGF-8. In the hypospadias group, it was limited to basal involvement (Epidermis Pattern 1) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).

Figure 6.

More than 50% involvement of keratinocytes (Epidermis Pattern 3) and clustered positive cells more than 50% of all visible dermal cells (Dermis Pattern C) for FGF-10 in the control group. In the hypospadias group, less than 50% of keratinocytes were involved (Epidermis Pattern 2) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).
More than 50% involvement of keratinocytes (Epidermis Pattern 3) and clustered positive cells more than 50% of all visible dermal cells (Dermis Pattern C) for FGF-10 in the control group. In the hypospadias group, less than 50% of keratinocytes were involved (Epidermis Pattern 2) and involvement of less than 50% of dermal cells (Dermis Pattern B) (IHC Staining, 200 X).

Quantitative scoring system of stratified squamous epithelium by immunohistochemistry for AR, ER-α and ER-β_

Score Grade
0 No involvement
+ Involvement in basal keratinocytes
++ Involvement limited to the lower 1/3 of the epidermis
+++ Involvement limited to the lower 2/3 of the epidermis
++++ Involvement in all epidermis layers

Quantitative scoring results of stratified squamous epithelium for AR and ERs (n=20)_

AR Estrogen Receptor-α Estrogen Receptor-β
Score Control Hypospadias Control Hypospadias Control Hypospadias
0 1 - 2 - - -
+ 18 - 17 - 18 -
++ 1 5 1 4 2 3
+++ - 15 - 16 - 17
++++ - - - - - -

Mean ± SD values of the markers in the groups for IHC evaluation_

Control Hypospadias
Androgen Receptor 2±0,32 3,75±0,44*
Estrogen Receptor-α 1,95±0,39 3,80±0,41*
Estrogen Receptor-β 2,10±0,30 3,85±0,36*
FGFR-2 Epidermis 3,80±0,41 2±0,32*
FGFR-2 Dermis 2,85±0,36 1,95±0,22*
FGF-8 Epidermis 3,75±0,44 2,15±0,48*
FGF-8 Dermis 2,30±0,47 1,85±0,36**
FGF-10 Epidermis 3,90±0,30 2,15±0,36*
FGF-10 Dermis 2,85±0,36 1,90±0,30*

Epidermis and dermis involvement of FGFR-2, FGF-8, FGF-10(n=20)_

FGFR-2 FGF-8 FGF-10
Pattern Control Hypospadias Control Hypospadias Control Hypospadias
Epidermis 1 - - - 16 - 17
2 4 - 5 4 2 3
3 16 5 15 - 18 -
Dermis A - 1 - 3 - 2
B 3 19 14 17 3 18
C 17 - 6 - 17 -
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