Mitochondrial DNA alternations in formalin-fixed paraffin-embedded tissue collected from dogs with malignant mammary gland tumours
Published Online: Jul 03, 2025
Page range: 215 - 224
Accepted: Mar 17, 2025
DOI: https://doi.org/10.2478/aspr-2025-0015
Keywords
© 2025 Angelika Tkaczyk-Wlizło et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Formalin-fixed paraffin embedded (FFPE) tissue is the most common histopathological technique for tumour assessment in the veterinary pathology. Due to easy availability of FFPE blocks, the aim of this retrospective study was to identify molecular defects caused by mutations in mitochondrial DNA (mtDNA) in cases of canine malignant mammary gland tumours (MGTs). DNA was isolated from 88 FFPE samples, then quantity and quality analyses were performed. Amplification was conducted on 10 selected fragments of 8 mitochondrial genes (