<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Microinjection is a micromanipulation technique that has been used in various fields and in a variety of species, including teleost fish, for over a hundred years. Microinjection is mainly used in genetic, molecular, and toxicological research. The paper describes an experimental study of a microinjection technique for the administration of Infectious Pancreatic Necrosis Virus (IPNV) in fertilized eggs of brook trout, <italic>Salvelinus fontinalis</italic> (Mitchill), and a rainbow trout <italic>Oncorhynchus mykiss</italic> (Walbaum) hybrid, <italic>Salvelinus fontinalis ♂ × Oncorhynchus mykiss</italic> ♀. The oocytes and embryos of these salmonids have complex structures that require modifying the method commonly used in zebrafish, <italic>Danio rerio</italic> (Hamilton), research. A modular apparatus and technique for injecting brook trout and rainbow brook trout fish embryos with IPNV are described. The modified microinjection method can be used successfully to administer to salmonid eggs infectious substances that simulate the vertical transmission of pathogens.</p>
</abstract>

Microinjection is a micromanipulation technique that has been used in various fields and in a variety of species, including teleost fish, for over a hundred years. Microinjection is mainly used in genetic, molecular, and toxicological research. The paper describes an experimental study of a microinjection technique for the administration of Infectious Pancreatic Necrosis Virus (IPNV) in fertilized eggs of brook trout, Salvelinus fontinalis (Mitchill), and a rainbow trout Oncorhynchus mykiss (Walbaum) hybrid, Salvelinus fontinalis ♂ × Oncorhynchus mykiss ♀. The oocytes and embryos of these salmonids have complex structures that require modifying the method commonly used in zebrafish, Danio rerio (Hamilton), research. A modular apparatus and technique for injecting brook trout and rainbow brook trout fish embryos with IPNV are described. The modified microinjection method can be used successfully to administer to salmonid eggs infectious substances that simulate the vertical transmission of pathogens.

Egg microinjections – method experimental viral infections in brook trout <italic>Salvelinus fontinalis</italic> (Mitchill, 1815) and rainbow brook trout <italic>Salvelinus fontinalis</italic> ♂ × <italic>Oncorhynchus mykiss</italic> ♀

Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine

University of Warmia and Mazury in Olsztyn

Department of Ichthyopathology and Fish Health Prevention

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine

Fisheries & Aquatic Life

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Egg microinjections – method experimental viral infections in brook trout  (Mitchill, 1815) and rainbow brook trout  ♂ ×  ♀

Microinjection is a micromanipulation technique that has been used in various fields and in a variety of species, including teleost fish, for over a hundred...

Egg microinjections – method experimental viral infections in brook trout Salvelinus fontinalis (Mitchill, 1815) and rainbow brook trout Salvelinus fontinalis ♂ × Oncorhynchus mykiss ♀

Published Online: Oct 20, 2022

Page range: 169 - 174

Received: May 22, 2022

Accepted: Sep 02, 2022

DOI: https://doi.org/10.2478/aopf-2022-0015

Keywords
Infectious Pancreatic Necrosis Virus, IPNV, microinjections in fish, trout hybrids, salmonid hybrids

© 2022 Karolina Duk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Egg microinjections – method experimental viral infections in brook trout Salvelinus fontinalis (Mitchill, 1815) and rainbow brook trout Salvelinus fontinalis ♂ × Oncorhynchus mykiss ♀

Published Online: Oct 20, 2022

Page range: 169 - 174

Received: May 22, 2022

Accepted: Sep 02, 2022

DOI: https://doi.org/10.2478/aopf-2022-0015

KeywordsInfectious Pancreatic Necrosis Virus, IPNV, microinjections in fish, trout hybrids, salmonid hybrids

© 2022 Karolina Duk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Infectious Pancreatic Necrosis Virus, IPNV, microinjections in fish, trout hybrids, salmonid hybrids