Cytotoxicity evaluation of different clear aligner materials using MTT analysis

1. Malkoc S, Corekci B, Ulker HE, Yalçin M, Şengün A. Cytotoxic effects of orthodontic composites. Angle Orthod 2010;80:759–64. Malkoc S Corekci B Ulker HE Yalçin M Şengün A . Cytotoxic effects of orthodontic composites . Angle Orthod 2010 ; 80 : 759 – 64 . 10.2319/092809-537.1896643820482365 Search in Google Scholar

2. Mockers O, Deroze D, Camps J. Cytotoxicity of orthodontic bands, brackets and archwires in vitro. Dent Mater 2002;18:311–7. Mockers O Deroze D Camps J . Cytotoxicity of orthodontic bands, brackets and archwires in vitro . Dent Mater 2002 ; 18 : 311 – 7 . 10.1016/S0109-5641(01)00055-0 Search in Google Scholar

3. Finke H, Koos B, Fischer-Brandies H, Es-Souni M. In vitro biocompatibility of orthodontic miniscrews with human gingival fibroblast and SAOS-2 osteoblast cultures. J Orofac Orthop 2018;79:328–36. Finke H Koos B Fischer-Brandies H Es-Souni M . In vitro biocompatibility of orthodontic miniscrews with human gingival fibroblast and SAOS-2 osteoblast cultures . J Orofac Orthop 2018 ; 79 : 328 – 36 . 10.1007/s00056-018-0143-330014178 Search in Google Scholar

4. Ihssen BA, Willmann JH, Nimer A, Drescher D. Effect of in vitro aging by water immersion and thermocycling on the mechanical properties of PETG aligner material. J Orofac Orthop 2019;80:292–303. Ihssen BA Willmann JH Nimer A Drescher D . Effect of in vitro aging by water immersion and thermocycling on the mechanical properties of PETG aligner material . J Orofac Orthop 2019 ; 80 : 292 – 303 . 10.1007/s00056-019-00192-831578595 Search in Google Scholar

5. Martina S, Rongo R, Bucci R, Razionale AV, Valletta R, D’Antò V. In vitro cytotoxicity of different thermoplastic materials for clear aligners. Angle Orthod 2019;89:942–5. Martina S Rongo R Bucci R Razionale AV Valletta R D’Antò V . In vitro cytotoxicity of different thermoplastic materials for clear aligners . Angle Orthod 2019 ; 89 : 942 – 5 . 10.2319/091718-674.1810916430945933 Search in Google Scholar

6. ISO—ISO 10993-5:2009—Biological evaluation of medical devices — Part 5: Tests for in vitro cytotoxicity. https://www.iso.org/ standard/36406.html. Accessed June 18, 2022. ISO—ISO 10993-5:2009—Biological evaluation of medical devices — Part 5: Tests for in vitro cytotoxicity https://www.iso.org/standard/36406.html. Accessed June 18, 2022. Search in Google Scholar

7. Vande Vannet B, Mohebbian N, Wehrbein H. Toxicity of used orthodontic archwires assessed by three-dimensional cell culture. Eur J Orthod 2006;28:426–32. Vande Vannet B Mohebbian N Wehrbein H . Toxicity of used orthodontic archwires assessed by three-dimensional cell culture . Eur J Orthod 2006 ; 28 : 426 – 32 . 10.1093/ejo/cjl00216901961 Search in Google Scholar

8. Ahrari F, Tavakkol Afshari J, Poosti M, Brook A. Cytotoxicity of orthodontic bonding adhesive resins on human oral fibroblasts. Eur J Orthod 2010;32:688–92. Ahrari F Tavakkol Afshari J Poosti M Brook A . Cytotoxicity of orthodontic bonding adhesive resins on human oral fibroblasts . Eur J Orthod 2010 ; 32 : 688 – 92 . 10.1093/ejo/cjq01920570954 Search in Google Scholar

9. Longo-Sorbello GSA, Saydam G, Banerjee D, Bertino JRCelis CE. Cytotoxicity and cell growth assays. Cell biology:a laboratuary handbook Vol 1 Burlington: Elsevier Inc; 2006pp. 315–24. Longo-Sorbello GSA Saydam G Banerjee D Bertino JR Celis CE . Cytotoxicity and cell growth assays . Cell biology:a laboratuary handbook Vol 1 Burlington : Elsevier Inc ; 2006 pp. 315 – 24 . 10.1016/B978-012164730-8/50039-3 Search in Google Scholar

10. Kumar P, Nagarajan A, Uchil PD. Analysis of cell viability by the MTT assay. Cold Spring Harb Protoc 2018;2018: 469–71. Kumar P Nagarajan A Uchil PD . Analysis of cell viability by the MTT assay . Cold Spring Harb Protoc 2018 ; 2018 : 469 – 71 . 10.1101/pdb.prot09550529858338 Search in Google Scholar

11. Eliades T, Pratsinis H, Athanasiou AE, Eliades G, Kletsas D. Cytotoxicity and estrogenicity of Invisalign appliances. Am J Orthod Dentofacial Orthop 2009;136:100–3. Eliades T Pratsinis H Athanasiou AE Eliades G Kletsas D . Cytotoxicity and estrogenicity of Invisalign appliances . Am J Orthod Dentofacial Orthop 2009 ; 136 : 100 – 3 . 10.1016/j.ajodo.2009.03.00619577155 Search in Google Scholar

12. Premaraj T, Simet S, Beatty M, Premaraj S. Oral epithelial cell reaction after exposure to Invisalign plastic material. Am J Orthod Dentofacial Orthop 2014;145:64–71. Premaraj T Simet S Beatty M Premaraj S . Oral epithelial cell reaction after exposure to Invisalign plastic material . Am J Orthod Dentofacial Orthop 2014 ; 145 : 64 – 71 . 10.1016/j.ajodo.2013.09.01124373656 Search in Google Scholar

13. ZENDURA Trademark of BAY MATERIALS, LLC—Serial Number 87878407—Alter. https://alter.com/trademarks/zendura-87878407 Accessed February 13, 2022. ZENDURA Trademark of BAY MATERIALS LLC—Serial Number 87878407—Alter https://alter.com/trademarks/zendura-87878407 Accessed February 13, 2022. Search in Google Scholar

14. Mudri NH, Abdullah LC, Aung MM, Salleh MZ, Biak DRA, Rayung M. Comparative study of aromatic and cycloaliphatic isocyanate effects on physico-chemical properties of bio-based polyurethane acrylate coatings. Polymers 2020;12:1494. Mudri NH Abdullah LC Aung MM Salleh MZ Biak DRA Rayung M . Comparative study of aromatic and cycloaliphatic isocyanate effects on physico-chemical properties of bio-based polyurethane acrylate coatings . Polymers 2020 ; 12 : 1494 . 10.3390/polym12071494740757932635384 Search in Google Scholar

15. Khadivi P, Salami-Kalajahi M, Roghani-Mamaqani H, Sofla RLM. Polydimethylsiloxane-based polyurethane/cellulose nanocrystal nanocomposites: from structural properties toward cytotoxicity. Silicon 2022;14:1695–703. Khadivi P Salami-Kalajahi M Roghani-Mamaqani H Sofla RLM . Polydimethylsiloxane-based polyurethane/cellulose nanocrystal nanocomposites: from structural properties toward cytotoxicity . Silicon 2022 ; 14 : 1695 – 703 . 10.1007/s12633-021-00970-3 Search in Google Scholar

16. Gregorí Valdés BS, Gomes CSB, Gomes PT, Ascenso JR, Diogo HP, Hermínio P, et al. New biocompatible polyurethanes: synthesis, structural characterization and cytotoxicity. 2018. doi: 10.20944/preprints201809.0445.v1. Gregorí Valdés BS Gomes CSB Gomes PT Ascenso JR Diogo HP Hermínio P New biocompatible polyurethanes: synthesis, structural characterization and cytotoxicity . 2018 doi: 10.20944/preprints201809.0445.v1. 10.20944/preprints201809.0445.v1 Search in Google Scholar

17. Lombardo L, Martines E, Mazzanti V, Arreghini A, Mollica F, Siciliani G. Stress relaxation properties of four orthodontic aligner materials: a 24-hour in vitro study. Angle Orthod 2017;87:11–8. Lombardo L Martines E Mazzanti V Arreghini A Mollica F Siciliani G . Stress relaxation properties of four orthodontic aligner materials: a 24-hour in vitro study . Angle Orthod 2017 ; 87 : 11 – 8 . 10.2319/113015-813.1838858827314603 Search in Google Scholar

18. Chemical and mechanical characteristics of contemporary thermoplastic orthodontic materials - PubMed. https://pubmed. ncbi.nlm.nih.gov/26999889/. Accessed December 26, 2021. Chemical and mechanical characteristics of contemporary thermoplastic orthodontic materials - PubMed https://pubmed.ncbi.nlm.nih.gov/26999889/. Accessed December 26, 2021. Search in Google Scholar

19. Product datasheet DURAN®. http://products.scheu-dental.com/ documents/5000/1-DOC/0/0/0/0/3/Datasheet-DUR A N_ Original_3055.pdf. Accessed June 18,2022. Product datasheet DURAN® http://products.scheu-dental.com/documents/5000/1-DOC/0/0/0/0/3/Datasheet-DURAN_Original_3055.pdf. Accessed June 18,2022. Search in Google Scholar

20. https://taglus.com/wp-content/themes/taglus/images/downloads/ Brochure.pdf. Accessed June 18, 2022. https://taglus.com/wp-content/themes/taglus/images/downloads/Brochure.pdf. Accessed June 18, 2022. Search in Google Scholar

21. Lu QW, Macosko CW. Comparing the compatibility of various functionalized polypropylenes with thermoplastic polyurethane (TPU). Polymer (Guildf) 2004;45:1981–91. Lu QW Macosko CW . Comparing the compatibility of various functionalized polypropylenes with thermoplastic polyurethane (TPU) . Polymer (Guildf) 2004 ; 45 : 1981 – 91 . 10.1016/j.polymer.2003.12.077 Search in Google Scholar

22. Liu CL, Sun WT, Liao W, Lu WX, Li QW, Jeong Y, et al. Colour stabilities of three types of orthodontic clear aligners exposed to staining agents. Int J Oral Sci 2016;8:246. Liu CL Sun WT Liao W Lu WX Li QW Jeong Y Colour stabilities of three types of orthodontic clear aligners exposed to staining agents . Int J Oral Sci 2016 ; 8 : 246 . 10.1038/ijos.2016.25516841327660048 Search in Google Scholar

23. Tamburrino F, D’Antò V, Bucci R, Alessandri-Bonetti G, Barone S, Razionale AV. Mechanical properties of thermoplastic polymers for aligner manufacturing: In vitro study. Dent J 2020;8: 2020;8:47. Tamburrino F D’Antò V Bucci R Alessandri-Bonetti G Barone S Razionale AV . Mechanical properties of thermoplastic polymers for aligner manufacturing: In vitro study . Dent J 2020 ; 8 : 2020 ; 8 : 47 . 10.3390/dj8020047734564232397575 Search in Google Scholar

24. And A, Zealand N. A perfect combination of elasticity, clarity and rigiditywww.taglus.com.auAccessed February 12, 2022. And A Zealand N . A perfect combination of elasticity, clarity and rigidity www.taglus.com.au Accessed February 12, 2022. Search in Google Scholar

25. Boubakri A, Elleuch K, Guermazi N, Ayedi HF. Investigations on hygrothermal aging of thermoplastic polyurethane material. Mater Des 2009;10:3958–65. Boubakri A Elleuch K Guermazi N Ayedi HF . Investigations on hygrothermal aging of thermoplastic polyurethane material . Mater Des 2009 ; 10 : 3958 – 65 . 10.1016/j.matdes.2009.05.038 Search in Google Scholar

26. Wataha JC. Predicting clinical biological responses to dental materials. Dent Mater 2012;28:23–40. Wataha JC . Predicting clinical biological responses to dental materials . Dent Mater 2012 ; 28 : 23 – 40 . 10.1016/j.dental.2011.08.59522192249 Search in Google Scholar

27. Devices@FDA. https://www.accessdata.fda.gov/scripts/cdrh/ devicesatfda/index.cfm?start_search=1&q=Y2xlY XIgY WxpZ 25lcg==&approval_date_from=05%2F25%2F1996&approval_ date_to=05%2F21%2F2022&sort=approvaldatedesc& pagenum=10. Accessed May 24, 2022. Devices@FDA https://www.accessdata.fda.gov/scripts/cdrh/devicesatfda/index.cfm?start_search=1&q=Y2xlYXIgYWxpZ25lcg==&approval_date_from=05%2F25%2F1996&approval_date_to=05%2F21%2F2022&sort=approvaldatedesc&pagenum=10. Accessed May 24, 2022. Search in Google Scholar

28. Recognized Consensus Standardshttps://www.accessdata.fda.gov/ scripts/cdrh/cfdocs/cfStandards/results.cfm?start_search=1& productcode=&category=&type=&title=&organization=& referencenumber=10993&regulationnumber=&effectivedatefrom= &effectivedateto=&PAGENUM=500. Accessed June 9, 2022. Recognized Consensus Standards https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfStandards/results.cfm?start_search=1&productcode=&category=&type=&title=&organization=&referencenumber=10993&regulationnumber=&effectivedatefrom=&effectivedateto=&PAGENUM=500. Accessed June 9, 2022. Search in Google Scholar

29. U.S. Department of Health & Human Services https://www. accessdata.fda.gov/CDRH510K/K081960.pdf. Accessed May 22, 2022. U.S. Department of Health & Human Services https://www.accessdata.fda.gov/CDRH510K/K081960.pdf. Accessed May 22, 2022. Search in Google Scholar

30. Gerard Bradley T, Teske L, Eliades G, Zinelis S, Eliades T. Do the mechanical and chemical properties of InvisalignTM appliances change after use? A retrieval analysis. Eur J Orthod 2016;38: 27–31. Gerard Bradley T Teske L Eliades G Zinelis S Eliades T . Do the mechanical and chemical properties of InvisalignTM appliances change after use? A retrieval analysis . Eur J Orthod 2016 ; 38 : 27 – 31 . 10.1093/ejo/cjv00325740599 Search in Google Scholar

31. Bakdach WMM, Haiba M, Hadad R. Changes in surface morphology, chemical and mechanical properties of clear aligners during intraoral usage: A systematic review and meta-analysis. Int Orthod 2022;20:100610. Bakdach WMM Haiba M Hadad R . Changes in surface morphology, chemical and mechanical properties of clear aligners during intraoral usage: A systematic review and meta-analysis . Int Orthod 2022 ; 20 : 100610 . 10.1016/j.ortho.2022.10061035090857 Search in Google Scholar