<abstract xmlns="http://www.w3.org/1999/xhtml">

The main objective of the current trial was to evaluate the beneficial influences of <italic>Origanum vulgare</italic> leaf extract (OVLE) on growth, some blood indices, redox status, and the expression of some growth and immune-related genes. Three thousand seven hundred and eighty Nile tilapia fingerlings were allocated to six equal groups. The first group served as a control and received a basal diet, while the other five groups were fed diets containing graded amounts of OVLE (0.1, 0.2, 0.3, 0.4, and 0.5%, respectively) and defined as OVLE0.1, OVLE0.2, OVLE0.3, OVLE0.4, and OVLE0.5 for 98 days. Growth performance and feed efficiency parameters were significantly improved in OVLE0.4 and OVLE0.5 groups compared with the control group. The hematological indices, specifically hemoglobin, red blood cell, and white blood cell count were significantly increased in the fish group fed a 4% OVLE-supplemented diet compared to other groups. Fish fed higher dietary inclusion levels of OVLE significantly increased total protein and albumin concentrations compared to other experimental groups. The OVLE0.4 and OVLE0.5 supplemented groups promoted innate immune response and phagocytic activity and stimulated the lipase enzyme activity of Nile tilapia. All estimated redox markers were significantly improved in the fish groups that received 4 or 5% OVLE compared with the control and other OVLE groups. The fish groups fed higher levels of OVLE significantly upregulated the expression of <italic>GH</italic>, <italic>IL-8</italic>, and <italic>IL-1β</italic> genes and downregulated the expression of the <italic>Hsp70</italic> gene. Furthermore, the intestinal histological features demonstrated normal structure in all OVLE-administered fish groups, with improved intestinal villus length compared to the untreated group. Finally, it could be concluded that including OVLE in the Nile tilapia diet at higher levels (0.4 up to 0.5%) could improve performance and general health status.
</abstract>

The main objective of the current trial was to evaluate the beneficial influences of Origanum vulgare leaf extract (OVLE) on growth, some blood indices, redox status, and the expression of some growth and immune-related genes. Three thousand seven hundred and eighty Nile tilapia fingerlings were allocated to six equal groups. The first group served as a control and received a basal diet, while the other five groups were fed diets containing graded amounts of OVLE (0.1, 0.2, 0.3, 0.4, and 0.5%, respectively) and defined as OVLE0.1, OVLE0.2, OVLE0.3, OVLE0.4, and OVLE0.5 for 98 days. Growth performance and feed efficiency parameters were significantly improved in OVLE0.4 and OVLE0.5 groups compared with the control group. The hematological indices, specifically hemoglobin, red blood cell, and white blood cell count were significantly increased in the fish group fed a 4% OVLE-supplemented diet compared to other groups. Fish fed higher dietary inclusion levels of OVLE significantly increased total protein and albumin concentrations compared to other experimental groups. The OVLE0.4 and OVLE0.5 supplemented groups promoted innate immune response and phagocytic activity and stimulated the lipase enzyme activity of Nile tilapia. All estimated redox markers were significantly improved in the fish groups that received 4 or 5% OVLE compared with the control and other OVLE groups. The fish groups fed higher levels of OVLE significantly upregulated the expression of GH, IL-8, and IL-1β genes and downregulated the expression of the Hsp70 gene. Furthermore, the intestinal histological features demonstrated normal structure in all OVLE-administered fish groups, with improved intestinal villus length compared to the untreated group. Finally, it could be concluded that including OVLE in the Nile tilapia diet at higher levels (0.4 up to 0.5%) could improve performance and general health status.

Oregano Leaf Extract Dietary Administration Modulates Performance, Redox Status, Intestinal Health, and Expression of Some Related Genes of Nile Tilapia (<italic>Oreochromis niloticus</italic> L.)

Department of Animal Production, Faculty of Agriculture

Department of Fish Nutrition, Sakha Research Unit

Department of Fish Production, Faculty of Agriculture

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Oregano Leaf Extract Dietary Administration Modulates Performance, Redox Status, Intestinal Health, and Expression of Some Related Genes of Nile Tilapia ( L.)

The main objective of the current trial was to evaluate the beneficial influences of Origanum vulgare leaf extract (OVLE) on growth, some blood indices, redox...

Oregano Leaf Extract Dietary Administration Modulates Performance, Redox Status, Intestinal Health, and Expression of Some Related Genes of Nile Tilapia (Oreochromis niloticus L.)

Published Online: Jan 23, 2024

Page range: 179 - 190

Received: Jan 03, 2023

Accepted: May 24, 2023

DOI: https://doi.org/10.2478/aoas-2023-0068

Keywords
herbal extract, immune-related genes, blood indices, antioxidative markers, histomorphology, growth performance

© 2024 Ahmed F. Fath El-Bab et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Oregano Leaf Extract Dietary Administration Modulates Performance, Redox Status, Intestinal Health, and Expression of Some Related Genes of Nile Tilapia (Oreochromis niloticus L.)

Published Online: Jan 23, 2024

Page range: 179 - 190

Received: Jan 03, 2023

Accepted: May 24, 2023

DOI: https://doi.org/10.2478/aoas-2023-0068

Keywordsherbal extract, immune-related genes, blood indices, antioxidative markers, histomorphology, growth performance

© 2024 Ahmed F. Fath El-Bab et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
herbal extract, immune-related genes, blood indices, antioxidative markers, histomorphology, growth performance