<abstract xmlns="http://www.w3.org/1999/xhtml">

The present study aimed to examine the effect of impaired progesterone (P4) synthesis, observed in gilts with gonadotropin-induced estrus, on the uterine expression of receptors important for pregnancy establishment. Twenty prepubertal gilts received 750 IU PMSG and 500 IU hCG 72 h later, while 18 prepubertal gilts in the control group were observed daily for estrus behavior. Gilts were inseminated in their first estrus and slaughtered on days 10, 12, and 15 of pregnancy to collect endometrial and myometrial tissues for mRNA analysis using real-time PCR. As we previously described, gilts with PMSG/hCG-induced estrus showed decreased luteal P4 synthesis on days 10 and 12 of pregnancy. PMSG/hCG treatment did not affect P4 receptor mRNA expression in either uterine tissue. In the endometrium, a greater mRNA transcript abundance of estrogen receptors (<italic>ESR1</italic> and <italic>ESR2</italic>), androgen receptor (<italic>AR</italic>), prostaglandin (PG) E2 receptors (<italic>PTGER2</italic> and <italic>PTGER4</italic>), PGF2α receptor (<italic>PTGFR</italic>), interleukin 6 receptor (<italic>IL6R</italic>), and tumor necrosis factor α receptors (<italic>TNFRSF1A</italic> and <italic>TNFRSF1B</italic>) was detected in gilts with natural than with PMSG/hCG-induced estrus (P&lt;0.05). In the myometrium, the mRNA expression of <italic>AR</italic>, <italic>PTGER2</italic>, and <italic>PTGFR</italic> was lower, while PGI2 receptor (<italic>PTGIR</italic>) transcript abundance was elevated in the gilts treated with PMSG/hCG as compared with the control animals (P&lt;0.05). In summary, a decreased luteal P4 level during the peri-implantation period in gonadotropin-stimulated pigs affects endometrial and myometrial receptor expression, with the endometrium being more sensitive to impaired P4 synthesis. Whether the observed changes alter uterine receptivity to local and systemic factors remains to be elucidated.
</abstract>

The present study aimed to examine the effect of impaired progesterone (P4) synthesis, observed in gilts with gonadotropin-induced estrus, on the uterine expression of receptors important for pregnancy establishment. Twenty prepubertal gilts received 750 IU PMSG and 500 IU hCG 72 h later, while 18 prepubertal gilts in the control group were observed daily for estrus behavior. Gilts were inseminated in their first estrus and slaughtered on days 10, 12, and 15 of pregnancy to collect endometrial and myometrial tissues for mRNA analysis using real-time PCR. As we previously described, gilts with PMSG/hCG-induced estrus showed decreased luteal P4 synthesis on days 10 and 12 of pregnancy. PMSG/hCG treatment did not affect P4 receptor mRNA expression in either uterine tissue. In the endometrium, a greater mRNA transcript abundance of estrogen receptors (ESR1 and ESR2), androgen receptor (AR), prostaglandin (PG) E2 receptors (PTGER2 and PTGER4), PGF2α receptor (PTGFR), interleukin 6 receptor (IL6R), and tumor necrosis factor α receptors (TNFRSF1A and TNFRSF1B) was detected in gilts with natural than with PMSG/hCG-induced estrus (P<0.05). In the myometrium, the mRNA expression of AR, PTGER2, and PTGFR was lower, while PGI2 receptor (PTGIR) transcript abundance was elevated in the gilts treated with PMSG/hCG as compared with the control animals (P<0.05). In summary, a decreased luteal P4 level during the peri-implantation period in gonadotropin-stimulated pigs affects endometrial and myometrial receptor expression, with the endometrium being more sensitive to impaired P4 synthesis. Whether the observed changes alter uterine receptivity to local and systemic factors remains to be elucidated.

Impaired luteal progesterone synthesis observed in gilts with PMSG/hCG-induced estrus affects the expression of steroid, prostaglandin, and cytokine receptors in the endometrium and myometrium during the peri-implantation period

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

The present study aimed to examine the effect of impaired progesterone (P4) synthesis, observed in gilts with gonadotropin-induced estrus, on the uterine...

Impaired luteal progesterone synthesis observed in gilts with PMSG/hCG-induced estrus affects the expression of steroid, prostaglandin, and cytokine receptors in the endometrium and myometrium during the peri-implantation period

Published Online: May 03, 2023

Page range: 439 - 448

Received: Dec 23, 2021

Accepted: Oct 07, 2022

DOI: https://doi.org/10.2478/aoas-2022-0076

Keywords
pig, progesterone, endometrium, myometrium, receptor expression

© 2023 Magdalena Szymanska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Supplementary Material Details

Impaired luteal progesterone synthesis observed in gilts with PMSG/hCG-induced estrus affects the expression of steroid, prostaglandin, and cytokine receptors in the endometrium and myometrium during the peri-implantation period

Published Online: May 03, 2023

Page range: 439 - 448

Received: Dec 23, 2021

Accepted: Oct 07, 2022

DOI: https://doi.org/10.2478/aoas-2022-0076

Keywordspig, progesterone, endometrium, myometrium, receptor expression

© 2023 Magdalena Szymanska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Supplementary Material Details

Keywords
pig, progesterone, endometrium, myometrium, receptor expression