Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year
access type Open Access

Effects of dietary halloysite supplementation on broiler chicken’s blood parameters, carcass and meat quality, and bone characteristics: a preliminary study

Published Online: 16 May 2022
Volume & Issue: AHEAD OF PRINT
Page range: -
Received: 03 Mar 2021
Accepted: 06 Apr 2022
Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year

The aim of this study was to determine the effect of halloysite supplementation to the complete diets for broiler chickens on selected blood parameters, carcass and organ characteristics, as well as bone and meat traits. The trial was conducted under experimental conditions in a large-scale poultry farm on 18,000 broiler chickens. The one-day broiler chicken Ross 308 chicks were randomly divided into two groups: control (C) and experimental (E) in an amount of 9,000 birds per group. In each group, chicks were separated into five pens (100 m2) with 1800 chicks per pen. The birds were fed granulated complete mixtures without (C group) or with halloysite (E group) distributed in an amount of 1% (based on fresh matter) from the 11 d of life. Analysis revealed that halloysite supplementation to the broiler diet at the level of 1% showed a trend (P = 0.056) towards higher final body weight, significantly improved carcass yield (P = 0.048), and reduced the relative weights of the liver and gizzard (P < 0.05). No differences (P > 0.05) were observed either in terms of the physicochemical parameters of the broiler chickens’ breast muscles, except a significantly lower b* value of the colour parameter (P = 0.048). In turn, in the blood serum, the addition of 1% halloysite significantly decreased (P < 0.05) the content of triglycerides and the total cholesterol and low-density lipoprotein fraction, but did not affect (P > 0.05) the high-density lipoprotein fraction, calcium, phosphorus or alkaline phosphate content. Tibia and femur characteristics (weight, length, relative bone weight, bone mineral density, bone mineral content, and breaking strength) were not affected (P > 0.05) by the usage of halloysite. In conclusion, supplementation of broiler diet with halloysite in the amount of 1% had beneficial effects on the carcass yield and health status of the animals, without any adverse effect on the bone parameters or meat quality of the broiler chickens.


Al-Beitawi N.A., Shaker M.M., Khaled N. El-Shuraydeh K.N., Bláha J. (2017). Effect of nanoclay minerals on growth performance, internal organs and blood biochemistry of broiler chickens compared to vaccines and antibiotics. J. Appl. Anim. Res., 45: 543–549. Search in Google Scholar

Augustyńska-Prejsnar A., Sokołowicz Z. (2014). Factors affecting the sensory quality of broiler chicken meat. Wiadomości Zoot., 2: 108–116. (In Polish) Search in Google Scholar

Aviagen. Ross Broiler Management Handbook. (2020). ROSS An Aviagen Brand. Available online: http://en.aviagen.com/assets/Tech_Center/Ross_PS/RossPSHandBook2018.pdf Search in Google Scholar

Banaszak M., Biesek J., Bogucka J., Dankowiakowska A., Olszewski D., Bigorowski B., Grabowicz M., Adamski M. (2020). Impact of aluminosilicates on productivity, carcass traits, meat quality, and jejunum morphology of broiler chickens. Poult. Sci., 99: 7169–7177. Search in Google Scholar

Barton C.D., Karathanasis A.D. (2002). Clay minerals. In Encyclopedia of soil science. Rattan Lal, comp., ed. Marcel Dekker: New York, USA, 187–192. Search in Google Scholar

Biesek J., Banaszak M., Adamski M. (2021). Ducks’ growth, meat quality, bone strength, and jejunum strength depend on zeolite in feed and long-term factors. Animals, 11: 1015. https://doi.org/10.3390/ani11041015.10.3390/ani11041015806715933916880 Search in Google Scholar

Bouderoua Y., Ait-Saada D., Selselet-Attou G., Mourot J., Perier C., Robin G. (2016). Effects of dietary addition of raw and treated calcium bentonite on growth, digesta characteristics, blood profiles and meat fatty acids composition of broilers chicks. Asian J. Anim. Vet. Adv., 11: 805–814. Search in Google Scholar

Chen Y., Cheng Y., Wang W., Wang A., Zhou Y. (2020). Protective effects of dietary supplementation with a silicate clay mineral (palygorskite) in lipopolysaccharide challenged broiler chickens at an early age. Anim. Feed Sci. Technol., 263: 114459. Search in Google Scholar

Cheng Y.F., Chen Y.P., Li X.H., Yang W.L., Wen C., Zhou Y.M. (2016). Effects of palygorskite inclusion on the growth performance, meat quality, antioxidant ability and mineral element content of broilers. Biol. Trace Elem. Res., 173: 194–201. Search in Google Scholar

CIE (1978). Recommendations on Uniform Color Spaces - Color Difference Equations, Psychometric Color Terms; Supplement No. 2 to CIE Publication No. 15 (E-1.3.1.); Bureau Central de la Commission Internationale de l’Eclairage: Paris, France. Search in Google Scholar

Durna Aydın Ö., Yıldız G., Güntürkün O.B., Bayraktaroğlu A.G. (2020). The use of glauconite as a feed additive in broiler nutrition and its effect on growth performance, intestinal histomorphology and biomechanical properties of bones. Kafkas Univ. Vet. Fak. Derg., 26: 343–349. Search in Google Scholar

Eleroglu H., Yalçın H., Yıldırım A. (2011). Dietary effects of ca-zeolite supplementation on some blood and tibial bone characteristics of broilers. S. Afr. J. Anim. Sci., 41: 319–330. Search in Google Scholar

Eraslan G., Eşsiz D., Akdoğan M., Şahindokuyucu F., Altıntaş L. (2005). The effects of aflatoxin and sodium bentonite combined and alone on some blood electrolyte levels in broiler chicks. Turk. J. Vet. Anim. Sci., 29: 601–605. Search in Google Scholar

Eser H., Songül S., Yalçin S., Sehu A. (2012). Effects of sepiolite usage in broiler diets on performance, carcass traits and some blood parameters. Kafkas Univ. Vet. Fak. Derg., 18: 313–318. Search in Google Scholar

Feldhusen F. (1994). Einfluüsse auf die postmortale Farbveränderung der Oberfläche von Schweinemuskulatur. Fleischwirtschaft, 74: 989–991. Search in Google Scholar

Friedewald W.T., Levyr I., Fredrickson D.S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultra-centrifuge. Clin. Chem., 18: 499–502. Search in Google Scholar

Janssen, W.M.M.A. (1989). European table of energy values for poultry feedstuffs, 3rd ed.; Subcommittee energy of the working group nr. 2 Nutrition of the European Federation of Branches of the World’s Poultry Science Association: Beekbergen, The Netherlands. Search in Google Scholar

Jarosz Ł., Stępień-Pyśniak D., Grądzki Z., Kapica M., Gacek A. (2017). The effect of feed supplementation with Zakarpacki zeolite (clinoptilolite) on percentages of T and B lymphocytes and cytokine concentrations in poultry. Poultry Sci., 96: 2091–2097. Search in Google Scholar

Kierończyk B., Rawski M., Józefiak D., Świątkiewicz S. (2017). Infectious and non-infectious factors associated with leg disorders in poultry – a review. Ann. Anim. Sci., 17: 645–669. Search in Google Scholar

Lemos M.J.D., Calixto L.F.L., Alves O.D.S., Souza D.S.D., Moura B.B., Reis T.L. (2015). Kaolin in the diet and its effects on performance, litter moisture and intestinal morphology of broiler chickens. Cienc. Rural, 45: 1835–1840. Search in Google Scholar

Li X., Chen Y.P., Cheng Y.F., Wen C., Zhou Y.M. (2017). Effects of dietary supplementation with yeast cell wall, palygorskite and their combination on the growth performance, meat quality, muscular antioxidant ability and mineral element content of broilers. Europ. Poult. Sci., 81. Search in Google Scholar

Lindahl G., Lundström K., Tornberg E. (2001). Contribution of pigment content, myoglobin forms and internal reflectance to the colour of pork loin and ham from pure breed pigs. Meat Sci., 59: 141–151. Search in Google Scholar

Lotfollahian H., Shariatmadari F., Shivazad M., Mirhadi A. (2004). Study on the effects of two kinds of natural zeolite in diets on blood biochemical parameters, relative weight of body organs and broilers performance. Pajouhesh-Va-Sazandegi, 64: 18–34. Search in Google Scholar

Makarski M., Niemiec T., Łozicki A., Pietrzak D., Adamczak L., Chmiel M., Florowski T., Koczoń P. (2020). The effect of silica-calcite sedimentary rock contained in the chicken broiler diet on the overall quality of chicken muscles. Open. Chem., 18: 215–225. Search in Google Scholar

Mancini R.A., Hunt M.C. (2005). Current research in meat colour. Meat Sci., 71: 100–121. Search in Google Scholar

Massaro M., Noto R., Riela S. (2020). Past, present and future perspectives on halloysite clay minerals. Molecules, 25: 4863. Search in Google Scholar

Mızrak C., Yenice E., Kahraman Z., Tunca M., Yıldırım U., Ceylan N. (2014). Effects of dietary sepiolite and mannanoligosaccharide supplementation on the performance, egg quality, blood and digestion characteristics of laying hens receiving aflatoxin in their feed. Ankara Üniv. Vet. Fak. Derg., 61: 65–71. Search in Google Scholar

Nadziakiewicz M., Kehoe S., Micek P. (2019). Physico-chemical properties of clay minerals and their use as a health promoting feed additive. Animals, 9(10): 714. https://doi.org/10.3390/ani910071410.3390/ani9100714682705931548509 Search in Google Scholar

Nadziakiewicz M., Lis M.W., Micek P. (2021). The effect of dietary halloysite supplementation on the performance of broiler chickens and broiler house environmental parameters. Animals, 11(7): 2040. https://doi.org/10.3390/ani1107204010.3390/ani11072040830021434359168 Search in Google Scholar

Ouachem D., Kaboul N., Meredef A., Abdessemed F., Ahmed Gaid Z. (2015a). Effects of clay on performance, moisture of droppings and health status of poultry: an overview. Worl. Poul. Sci. Jour., 71: 184–189.10.1017/S004393391500015X Search in Google Scholar

Ouachem D., Meredef A., Kaboul N. (2015b). The marl and kaolin in broiler diet: Effects on the bone weight and the cutting yield. The 6th International Seminar on Tropical Animal Production Integrated Approach in Developing Sustainable Tropical Animal Production. October 20-22, Yogyakarta, Indonesia, 72–75. Search in Google Scholar

Ouhida I., Pérez J.F., Gasa J. (2000). Sepiolite (Exal) decreases microbial colonization in the gastrointestinal tract of young broilers fed barley-wheat based diets. Arch. Zoot., 49: 501–504. Search in Google Scholar

Owen O.J., Nodu M.B. (2012). The effects of dietary kaolin (clay) as feed additive on the growth performance of broiler chickens. Green. J. Agric. Sci., 2: 233–236. Search in Google Scholar

Park J.H., Lee D.B., Kim S.H., Shin W.J., Ryu K.S. (2002). Effect of dietary supplementation of artificial and natural zeolites on performance and intestinal microbes of broiler chicks. Korean J. Poult. Sci., 29: 101–107. Search in Google Scholar

PN-77/A-82058 (1977). Meat and meat products. Determination of pH of meat. Search in Google Scholar

Połtowicz K. (2000). Effect of breast muscles’ initial pH on selected indicators of meat of broiler chickens of three genotypes. Rocz. Nauk. Zoot. Suppl., 8: 161–165. (In Polish) Search in Google Scholar

Prvulovic D., Kojic D., Grubor-Lajsic G., Kosarcic S. (2008). The effects of dietary inclusion of hydrated aluminosilicate on performance and biochemical parameters of broiler chickens. Turk. J. Vet. Anim. Sci., 32: 183–189. Search in Google Scholar

Rath N.C., Huff G.R., Huff W.E., Balog J.M. (2000). Factors regulating bone maturity and strength in poultry. Poult. Sci., 79: 1024–1032. Search in Google Scholar

Saçakli P., Calik A., Bayraktaroğlu A.G., Ergün A., Şahan Ö., Özaydin S. (2015). Effect of clinoptilolite and/or phytase on broiler growth performance, carcass characteristics, intestinal histomorphology and tibia calcium and phosphorus levels. Kafkas Univ. Vet. Fak. Derg., 21: 729–737. Search in Google Scholar

Safaei Katouli M., Boldaji F., Dastar B., Hassani S. (2012). Growth response and tibia bone characteristics in broilers fed diets containing kaolin, bentonite and zeolite. J. Anim. Feed Sci., 21: 334–344. Search in Google Scholar

Safaei M., Boldaji F., Dastar B., Hassani S., Taran, M. (2014). Economic analysis using silicate minerals in broiler chickens diets. Anim. Biol. Anim. Husb., 6: 2016–2023. Search in Google Scholar

Safari M.H., Shams S.M., Shargh, M.S., Amini A., Tatar A. (2015). Effects of different levels of natural glauconite and zeolite on performance, tibia bone characteristics and blood parameters of broiler chicken. Anim. Sci. J., 105: 167–178. Search in Google Scholar

Schneider A.F., Zimmermann O.F., Gewehr C.E. (2017). Zeolites in poultry and swine production. Cienc. Rural, 47: 1–8. Search in Google Scholar

Shi Y., Xu Z., Sun Y., Wang C., Feng J. (2009). Effects of different types of montmorillonite on growth performance and serum profiles of broiler chicks during aflatoxicosis. Turk. J. Anim. Sci., 33: 15–20. Search in Google Scholar

Shim M.Y., Karnuah A.B., Mitchell A.D., Anthony N.B., Pesti G.M., Aggrey S.E. (2012). The effects of growth rate on leg morphology and tibia breaking strength, mineral density, mineral content, and bone ash in broilers. Poult. Sci., 91: 1790–1795. Search in Google Scholar

Slamova R., Trckova M., Vondruskava H., Zraly Z., Pavlik I. (2011). Clay minerals in animal nutrition. Appl. Clay Sci., 51: 395–398. Search in Google Scholar

Subramaniam M., Kim I.H. (2015). Clays as dietary supplements for swine: A review. J. Anim. Sci. Biotech., 6: 2–9. Search in Google Scholar

Tatar A., Boldaji F., Dastar B., Hassani S., Yalçın S. (2012). Effects of dietary supplementation with perlite and zeolite on performance, litter quality and carcass characteristics of broilers from 7–42 days of age. Int. Res. J. Appl. Basic Sci., 3: 1148–1154. Search in Google Scholar

Tatar A., Boldaji F., Dastar B., Yaghobfar A. (2008). Effects of perlite and zeolite on serum characteristics, bone ash, gut pH and performance of broiler chickens. In: Paper presented at the 13th Asian-Aust. Anim. Scie. Assoc. Congress. Hanoi, Vietnam, 273. Search in Google Scholar

Trckova M., Matlova L., Dvorska L., Pavlik I. (2004). Kaolin, bentonite, and zeolites as feed supplements for animals: health advantages and risks. Vet. Med. Czech., 49: 389–399. Search in Google Scholar

Uzunoğlu K., Yalçın S. (2019). Effects of dietary supplementation of betaine and sepiolite supplementation on performance and intestinal health in broilers. Ankara Üniv. Vet. Fak. Derg., 66: 221–230. Search in Google Scholar

Watkins K.L., Southern L.L. (1992). Effect of dietary sodium zeolite A and graded levels of calcium and phosphorous on growth, plasma, and tibia characteristics of chicks. Poult. Sci., 71: 1048–1058. Search in Google Scholar

Wawrzyniak A., Kapica M., Stępień-Pyśniak D., Szewerniak R., Olejarska A., Jarosz Ł. (2017). Effect of feeding transcarpathian zeolite on gastrointestinal morphology and function in broiler chickens. Rev. Bras. Cienc. Avic., 19: 737–746. Search in Google Scholar

Yalçın S., Bilgili S.E., McDaniel G. R. (1995). Sodium zeolite A: influence on broiler carcass yields and tibia characteristics. Appl. Poult. Sci., 4: 81–88. Search in Google Scholar

Yalçin S., Gebeġ E.S., Ġahin A., Duyum H.M., Escribano F., Ceylan A. (2017). Sepiolite as a feed supplement for broilers. Appl. Clay Sci., 148: 95–102. Search in Google Scholar

Yang W.L., Chen Y.P., Cheng Y.F., Li X.H., Zhang R.Q., Wen C., Zhou Y. (2016). An evaluation of zinc bearing palygorskite inclusion on the growth performance, mineral content, meat quality, and antioxidant status of broilers. Poult. Sci., 95: 878–886. Search in Google Scholar

Zaefarian F., Abdollahi M.R., Cowieson A., Ravindran V. (2019). Avian liver: The forgotten organ. Animals, 9(2): 63. https://doi.org/10.3390/ani902006310.3390/ani9020063640685530781411 Search in Google Scholar

Zhang L., Yan R., Zhang R., Wen C., Zhou Y. (2017). Effect of different levels of palygorskite inclusion on pellet quality, growth performance and nutrient utilization in broilers. Anim. Feed Sci. Technol. 223: 73–81. Search in Google Scholar

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