Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year
access type Open Access

Dietary L-glutamine affects eggshell quality in the post-peak laying period

Published Online: 02 May 2022
Volume & Issue: AHEAD OF PRINT
Page range: -
Received: 21 Dec 2021
Accepted: 15 Feb 2022
Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year

In the current study the hypothesis that 1.0% dietary inclusion of glutamine (Gln), a conditionally essential amino acid that influences protein synthesis and shows anti-osteoporotic effect, can influence eggshell quality was tested on laying hens in the post-peak laying period. A 30-week-old Bovans Brown hens were randomly assigned to control group or group supplemented with Gln in the form of alpha-ketoglutarate (10 g/kg) with 12 replicate cages (2 hens/cage) in each group. The experimental period lasted for 30 weeks, from the 31st to the 60th week of age of hens, when eggs were collected and selected eggshell quality indices were determined. While Gln supplementation had no effect on egg geometry and eggshell cracking force, the analysis of eggshell quality showed an increase of the eggshell thickness (P<0.01), porosity (P<0.01), eggshell specific surface (P<0.001) and positive changes in other mechanical parameters (decrease of eggshell stiffness and Young’s modulus, P<0.05 and P<0.01, respectively; increase of work needed to crack the eggshell, P<0.05). Changes in eggshell mineral composition, including an increase of Ca content (P<0.001), were also observed. In conclusion, the current study showed a beneficial effect of Gln on the eggshell thickness, calcification, and some mechanical parameters.

Ahmed A.M.H., Rodrigez-Navarro A.B., Vidal M.L., Gautron J., Garcia-Ruiz J.M., Nys Y. (2005). Changes in eggshell mechanical properties, crystallographic texture and in matrix proteins induced by moult in hens. Br. Poult. Sci., 46: 268–279. Search in Google Scholar

Altuntas E., Sekeroglu A. (2008). Effect of egg shape index on mechanical properties of chicken eggs. J. Food. Eng., 85: 606–612. Search in Google Scholar

Ar A., Paganelli C.V., Reeves R.B., Greene D.G., Rahn H. (1974). The avian egg: Water vapor conductance, shell thickness, and functional pore area source. Condor, 76: 153–158. Search in Google Scholar

Athanasiadou D., Jiang W., Goldbaum D., Saleem A., Basy K., Pacella M.S., Böhm C.F., Chromik R.R., Hincke M.T., Rodríguez-Navarro A.B., Vali H., Wolf S.E., Gray J.J., Buy K.H., McKee M.D. (2018). Nanostructure, osteopontin, and mechanical properties of calcitic avian eggshell. Sci. Adv., 4: eaar3219. Search in Google Scholar

Bain M.M. (1990). Eggshell strength: A mechanical/ultrastructural evaluation. Ph.D. thesis, University of Glasgow, UK. Search in Google Scholar

Bartell S.M. , Batal A.B. (2007). The effect of supplemental glutamine on growth performance, development of the gastrointestinal tract, and humoral immune response of broilers. Poultry Sci., 86: 1940–1947. Search in Google Scholar

Burrin D.G., Stoll B. (2009). Metabolic fate and function of dietary glutamate in the gut. Am. J. Clin. Nutr., 90: 850S–856S. Search in Google Scholar

De Ketelaere B., Govaerts T., Coucke P., Dewil E., Visscher J., Decuypere E., De Baerdemaeker J. (2002). Measuring the eggshell strength of 6 different genetic strains of laying hens: Techniques and comparisons. Br. Poult. Sci., 43: 238–244. Search in Google Scholar

Dos Santos F., Brandão M., Da Silva C., Machado L.D.S., Soares M., Barreto M., Nascimento E.D., Pereira V. (2014). Eggshell apex abnormalities in a free-range hen farm with Mycoplasma synoviae and infectious Bronchitis virus in Rio de Janeiro State, Brazil. Braz. J. Poult. Sci., 16: 101–103. Search in Google Scholar

Drabik K., Batkowska J., Vasiukov K., Putam A. (2020). The impact of eggshell colour on the quality of table and hatching eggs derived from Japanese quail. Animals, 10: 264. Search in Google Scholar

EFSA, European Food Safety Authority (2020). Safety and efficacy of L-glutamine produced using Corynebacterium glutamicum NITE BP-02524 for all animal species. EFSA J., 18: 6075. Search in Google Scholar

EU, European Union, Commission Regulation (EC) No 152/2009 of 27 January 2009 laying down the methods of sampling and analysis for the official control of feed. Off. J. Eur. Union, 54: 1–130. Search in Google Scholar

Festin M.F.W. (2018). On determining sample size in experiments involving laboratory animals. Lab. Anim., 52: 341–350. Search in Google Scholar

Fritz C.O., Morris P.E., Richler J.J. (2012). Effect size estimates: Current use, calculations, and interpretation. J. Exp. Psychol., 141: 2–18. Search in Google Scholar

Gholipour V., Camani M., Aghdam Shahryar H., Sadeghi A.A., Aminafshar M. (2017). Effects of dietary L-glutamine supplemented on performance, egg quality, fertility and some blood biochemical parameters in Guinea fowls (Numida meleagridis). Kafkas Univ. Vet. Fak. Derg., 23: 903–910. Search in Google Scholar

Gholipour V., Camani M., Aghdam Shahryar H., Sadeghi A.A., Aminafshar M. (2019). Effects of dietary L-glutamine supplement on performance, characteristics of the carcase and intestinal morphometry in guinea fowl chickens (Numida meleagris). Ital. J. Anim. Sci., 18: 513–521. Search in Google Scholar

Goran G.V., Crivineanu V., Tudoreanu L., Udrea D. (2010). Dynamics of some mineral elements in hen eggs. Bull. Univ. Agric. Sci. Vet. Med. Cluj-Napoca Vet. Med., 67: 88–95. Search in Google Scholar

Hamilton R.M.G. (1982). Methods and factors that affect the measurement of egg shell quality. Poultry Sci., 61: 2022–2039. Search in Google Scholar

Jazideh F., Farhoomand P., Daneshyar M., Najafi G. (2014). The effects of dietary glutamine supplementation on growth performance and intestinal morphology of broiler chickens reared under hot conditions. Turk. J. Vet. Anim. Sci., 38: 264–270. Search in Google Scholar

Kibala L., Rozempolska-Rucińska I., Kasperek K., Zięba G., Łukaszewicz M. (2015). Ultrasonic eggshell thickness measurement for selection of layers. Poultry Sci., 94: 2360– 2362. Search in Google Scholar

Luquetti B.C., Fernandez Alarcon M.F., Lunedo R., Borges Campos D.M., Furlan R.L., Macari M. (2016). Effects of glutamine on performance and intestinal mucosa morphometry of broiler chickens vaccinated against coccidiosis. Sci. Agricol., 73: 322–327. Search in Google Scholar

Mabe I., Rapp C., Bain M.M., Nys Y. (2003). Supplementation of a corn-soybean meal diet with manganese, copper, and zinc from organic or inorganic sources improves eggshell quality in aged laying hens. Poultry Sci., 82: 1903–1913. Search in Google Scholar

Mohsenin N.N. (1986). Physical properties of plant and animal materials: structure, physical characteristics and mechanical properties. 2nd ed. Gordon and Breach, London. Search in Google Scholar

Mori H., Takaya M., Nishimura K., Goto T. (2020). Breed and feed affect amino acid contents of egg yolk and eggshell color in chickens. Poultry Sci., 99: 172–178. Search in Google Scholar

Mortola J.P. (2009). Gas exchange in avian embryos and hatchlings. Comp. Biochem. Physiol. A, 153: 359–377. Search in Google Scholar

Murray R.K., Granner D.K., Mayes P.A., Rodwell V.W. (2000). Harper’s Biochemistry. 25th ed. McGraw-Hill Medical. Search in Google Scholar

Narushin V.G. (2005). Egg geometry calculation using the measurements of length and breadth. Poultry Sci., 84: 482–484. Search in Google Scholar

Nys Y., Le Roy N. (2018). Calcium homeostasis and eggshell biomineralization in female chicken. In: Vitamin D. Volume 1: Biochemistry, physiology and diagnostics, Feldman D. (ed.). 4th Ed., Academic Press, pp. 361–382.10.1016/B978-0-12-809965-0.00022-7 Search in Google Scholar

Orczewska-Dudek S., Pietras M., Puchała M., Nowak J. (2020). Camelina sativa oil and camelina cake as sources of polyunsaturated fatty acids in the diets of laying hens: effect on hen performance, fatty acid profile of yolk lipids, and egg sensory quality. Ann. Anim. Sci., 20: 1365–1377. Search in Google Scholar

Park J.A., Shon S.H. (2018). The influence of hen aging on eggshell ultrastructure and shell mineral components. Korean J. Food Sci. Anim. Res., 38: 1080–1091. Search in Google Scholar

Radwan L.M., Fathi M.M., Galal A., Zein El-Dein A. (2010). Mechanical and ultrastructural properties of eggshell in two Egyptian native breeds of chicken. Int. J. Poult. Sci., 9: 77–81. Search in Google Scholar

Rodruguez-Navarro A., Kalin O., Nys Y., Garia-Ruiz J.M. (2002). Influence of the microstructure on the shell strength of eggs laid by hens of different ages. Br. Poult. Sci., 43: 395–40310.1080/0007166012010367512195799 Search in Google Scholar

Roudybush T., Hoffman L., Rahn H. (1980). Conductance, pore geometry, and water loss of eggs of Cassin’s Auklet. Condor, 82: 105–106. Search in Google Scholar

Sakamoto M.I., Murakami A.E., Silveira T.G.V., Fernandes J.I.M., De Oliveira C.A.L. (2006). Influence of glutamine and vitamin E on the performance and the immune responses of broiler chickens. Braz. J. Poult. Sci., 8: 243–249. Search in Google Scholar

Smulikowska S., Rutkowski A. (2018). Editors. Recommended allowances and nutritive value of feedstuffs – poultry feeding standards (in Polish). 5th ed. The Kielanowski Institute of Animal Physiology and Nutrition, Jabłonna, Poland, PAS. Search in Google Scholar

Sokołowicz Z., Krawczyk J., Dykiel M. (2018). The effect of the type of alternative housing system, genotype and age of laying hens on egg quality. Ann. Anim. Sci., 18: 541–555. Search in Google Scholar

Solomon S.E. (2010). The eggshell: strength, structure and function. Br. Poult. Sci., 51(supl1): 52–59. Search in Google Scholar

Soltan M.A. (2008). Effect of dietary organic acid supplementation on egg production, egg quality and some blood serum parameters in laying hens. Int. J. Poult. Sci., 7: 613–621. Search in Google Scholar

Soltan M.A. (2009). Influence of dietary glutamine supplementation on growth performance, small intestine morphology, immune response and some blood parameters of broiler chickens. Int. J. Poult. Sci., 8: 60–68. Search in Google Scholar

Świątkiewicz S., Arczewska-Włosek A., Krawczyk J., Szczurek W., Puchała M., Józefiak D. (2018). Effect of selected feed additives on egg performance and eggshell quality in laying hens fed a diet with standard or decreased calcium content. Ann. Anim. Sci., 18: 167–183. Search in Google Scholar

Świątkiewicz S., Arczewska-Włosek A., Szczurek W., Calik J., Bederska-Łojewska D., Orczewska-Dudek S, Muszyński S., Tomaszewska E., Józefiak D. (2020). Algal oil as source of polyunsaturated fatty acids in laying hens nutrition: Effect on egg performance, egg quality indices and fatty acid composition of egg yolk lipids. Ann. Anim. Sci., 20: 961– 973. Search in Google Scholar

Tomaszewska E., Świątkiewicz S., Arczewska-Włosek A., Wojtysiak D., Dobrowolski P., Domaradzki P., Świetlicka I., Donaldson J., Hułas-Stasiak M., Muszyński S. (2020). Alpha-ketoglutarate: An effective feed supplement in improving bone metabolism and muscle quality of laying hens: A preliminary study. Animals, 10: 2420. Search in Google Scholar

Tyler C. (1953). Studies on egg shells. II – A method for marking and counting pores. J. Sci. Food Agric., 4: 266–272. Search in Google Scholar

Xiao D., Zeng L., Yao K., Kong X., Wu G., Yin Y. (2016). The glutamine-alpha-ketoglutarate (AKG) metabolism and its nutritional implications. Amino Acids, 48: 2067–2080. Search in Google Scholar

Xiao J.F., Zhang Y.N., Wu S.G., Zhang H.J., Yue H.Y., Qi G.H. (2014). Manganese supplementation enhances the synthesis of glycosaminoglycan in eggshell membrane: A strategy to improve eggshell quality in laying hens. Poultry Sci., 93: 380–388. Search in Google Scholar

Xue G.D., Barekatain R., Wu S.B., Choct M., Swick R.A. (2018). Dietary L-glutamine supplementation improves growth performance, gut morphology, and serum biochemical indices of broiler chickens during necrotic enteritis challenge. Poultry Sci., 97: 1334–1341. Search in Google Scholar

Zavarize K.C., Sartori J.R., Pezzato A.C., Garcia E.A., Cruz V.C. (2011). Glutamine in diet of laying hens submitted to heat stress and thermoneutrality. Ci. Anim. Bras., 12: 400–406. Search in Google Scholar

Zhang Y., Li H., Zhang Y., Song F. (2018). Statistical optimization and batch studies on adsorption of phosphate using Al-eggshell. Adsorp. Sci. Technol., 36: 026361741774079. Search in Google Scholar

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