<abstract xmlns="http://www.w3.org/1999/xhtml">

An 8-week feeding trial was conducted to examine the efficacy of stachyose as a prebiotic on immune parameters, antioxidant-/immune-related genes’ expression, and lipid metabolism of zebrafish. Three hundred zebrafish (0.45 ± 0.08 g) were fed four diets containing different stachyose levels at 0, 1, 2 and 4 g kg−1, respectively. After eight weeks of the feeding trial, immunity, antioxidant defence and lipid metabolism were tested. It was observed that the addition of stachyose to the diet induced no significant influence (P>0.05) in SOD, GPX, and CAT, gene’s expression, compared to the control diet. The inclusion of stachyose resulted in no significant changes in immune gene expression (Lyz, IL-1, IL-6, and TNF) in zebrafish (P>0.05) compared to the control diet. Total cholesterol, triglyceride and LDL (low-density lipoprotein) significantly (P&lt;0.05) decreased with the addition of 2 and 4 g kg−1 stachyose, while fish fed the control diet and 1 g.kg−1 recorded the highest significant value of LDL (P&lt;0.05). Fish fed diet, either control diet or diet supplemented with 0.5 g kg−1 stachyose, recorded the lowest HDL value (P&lt;0.05) compared to other treatments. In conclusion, stachyose can be potentially used as a feed additive to modulate lipid metabolism. However, this prebiotic did not benefit immune parameters and antioxidant defence.
</abstract>

An 8-week feeding trial was conducted to examine the efficacy of stachyose as a prebiotic on immune parameters, antioxidant-/immune-related genes’ expression, and lipid metabolism of zebrafish. Three hundred zebrafish (0.45 ± 0.08 g) were fed four diets containing different stachyose levels at 0, 1, 2 and 4 g kg−1, respectively. After eight weeks of the feeding trial, immunity, antioxidant defence and lipid metabolism were tested. It was observed that the addition of stachyose to the diet induced no significant influence (P>0.05) in SOD, GPX, and CAT, gene’s expression, compared to the control diet. The inclusion of stachyose resulted in no significant changes in immune gene expression (Lyz, IL-1, IL-6, and TNF) in zebrafish (P>0.05) compared to the control diet. Total cholesterol, triglyceride and LDL (low-density lipoprotein) significantly (P<0.05) decreased with the addition of 2 and 4 g kg−1 stachyose, while fish fed the control diet and 1 g.kg−1 recorded the highest significant value of LDL (P<0.05). Fish fed diet, either control diet or diet supplemented with 0.5 g kg−1 stachyose, recorded the lowest HDL value (P<0.05) compared to other treatments. In conclusion, stachyose can be potentially used as a feed additive to modulate lipid metabolism. However, this prebiotic did not benefit immune parameters and antioxidant defence.

The effects of dietary stachyose as prebiotic on immunity and antioxidant related genes’ expression and lipid metabolism in zebrafish (<italic>Danio rerio</italic>)

Department of Fisheries, Faculty of Fisheries and Environmental Sciences

Gorgan University of Agricultural Sciences and Natural Resources

Radin Makian Azma Mehr Ltd., Radinmehr Veterinary Laboratory

Fish Nutrition Research Laboratory, Department of Animal Production, Faculty of Agriculture

Innovative Aqriculture Research Center, Faculty of Agriculture

Department of Animal and Aquatic Sciences, Faculty of Agriculture

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

The effects of dietary stachyose as prebiotic on immunity and antioxidant related genes’ expression and lipid metabolism in zebrafish ()

An 8-week feeding trial was conducted to examine the efficacy of stachyose as a prebiotic on immune parameters, antioxidant-/immune-related genes’ expression,...

The effects of dietary stachyose as prebiotic on immunity and antioxidant related genes’ expression and lipid metabolism in zebrafish (Danio rerio)

Published Online: Jul 19, 2022

Page range: 1097 - 1104

Received: Sep 22, 2021

Accepted: Feb 15, 2022

DOI: https://doi.org/10.2478/aoas-2022-0019

Keywords
stachyose, prebiotic, immune system, antioxidant defence, lipid metabolism

© 2022 Hesamaddin Shirzad Aski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

The effects of dietary stachyose as prebiotic on immunity and antioxidant related genes’ expression and lipid metabolism in zebrafish (Danio rerio)

Published Online: Jul 19, 2022

Page range: 1097 - 1104

Received: Sep 22, 2021

Accepted: Feb 15, 2022

DOI: https://doi.org/10.2478/aoas-2022-0019

Keywordsstachyose, prebiotic, immune system, antioxidant defence, lipid metabolism

© 2022 Hesamaddin Shirzad Aski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
stachyose, prebiotic, immune system, antioxidant defence, lipid metabolism