<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The present study aimed to identify the carotenoid content and evaluate the antioxidant activities of spirulina, <italic>Arthrospira platensis</italic> (<italic>in vitro</italic>), and the effect of its supplementation to a vegetarian diet on growth performance, feed utilization, body proximate composition and physiological status of Nile tilapia, <italic>Oreochromis niloticus</italic> (<italic>in vivo</italic>). The carotenoid content of spirulina was identified by UPLC-MS/MS and showed that <italic>trans</italic> and <italic>cis</italic> β-carotene were the major carotenoids (88.3%) followed by β-cryptoxanthin and zeaxanthin. The antioxidant activity of spirulina was determined in water and crude carotenoid extracts by 2,2-diphenyl-1-picryhydrazyl (DPPH) scavenging assay, both extracts showed a significant free radical scavenging capacity. In addition, Nile tilapia fry (0.83 ± 0.01 g) was fed a vegetarian diet supplemented with different levels of spirulina for 12 weeks. The results revealed that the highest growth performance and nutrient utilization were recorded with 0.5% spirulina and the peak response determined at 0.63%-0.65% using polynomial second order regression. Also, the hemoglobin content improved in a quadratic regression model with the peak at 0.67% spirulina. Plasma total protein and lipid contents increased significantly with spirulina levels over 0.50%. Moreover, the aminopeptidase activities and glucose level decreased significantly with increasing spirulina levels. The current study recommended the supplementation of the Nile tilapia vegetarian diet with spirulina at levels of 0.63-0.65% for better growth performance and physiological status.</p>
</abstract>

The present study aimed to identify the carotenoid content and evaluate the antioxidant activities of spirulina, Arthrospira platensis (in vitro), and the effect of its supplementation to a vegetarian diet on growth performance, feed utilization, body proximate composition and physiological status of Nile tilapia, Oreochromis niloticus (in vivo). The carotenoid content of spirulina was identified by UPLC-MS/MS and showed that trans and cis β-carotene were the major carotenoids (88.3%) followed by β-cryptoxanthin and zeaxanthin. The antioxidant activity of spirulina was determined in water and crude carotenoid extracts by 2,2-diphenyl-1-picryhydrazyl (DPPH) scavenging assay, both extracts showed a significant free radical scavenging capacity. In addition, Nile tilapia fry (0.83 ± 0.01 g) was fed a vegetarian diet supplemented with different levels of spirulina for 12 weeks. The results revealed that the highest growth performance and nutrient utilization were recorded with 0.5% spirulina and the peak response determined at 0.63%-0.65% using polynomial second order regression. Also, the hemoglobin content improved in a quadratic regression model with the peak at 0.67% spirulina. Plasma total protein and lipid contents increased significantly with spirulina levels over 0.50%. Moreover, the aminopeptidase activities and glucose level decreased significantly with increasing spirulina levels. The current study recommended the supplementation of the Nile tilapia vegetarian diet with spirulina at levels of 0.63-0.65% for better growth performance and physiological status.

The Evaluation of <italic>Arthrospira platensis</italic> Bioactivity and their Dietary Supplementation to Nile Tilapia Vegetarian Diet on Growth Performance, Feed Utilization, Body Composition and Hemato-Biochemical Parameters

Department of Animal and Fish Production, College of Agricultural and Food Sciences

Department of Fish and Animal Production, Faculty of Agriculture (Saba Basha)

Department of Fish and Animal Production, Faculty of Agriculture (El Shatby)

Agricultural Biochemistry Department, Faculty of Agriculture

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

The Evaluation of  Bioactivity and their Dietary Supplementation to Nile Tilapia Vegetarian Diet on Growth Performance, Feed Utilization, Body Composition and Hemato-Biochemical Parameters

The present study aimed to identify the carotenoid content and evaluate the antioxidant activities of spirulina, Arthrospira platensis (in vitro), and the...

The Evaluation of Arthrospira platensis Bioactivity and their Dietary Supplementation to Nile Tilapia Vegetarian Diet on Growth Performance, Feed Utilization, Body Composition and Hemato-Biochemical Parameters

Published Online: Aug 05, 2021

Page range: 1061 - 1080

Received: Oct 08, 2020

Accepted: Jan 18, 2021

DOI: https://doi.org/10.2478/aoas-2021-0003

Keywords
carotenoids identification, DPPH assay, growth performance, physiological response, Oreochromis niloticus

© 2021 Abdallah Tageldein Mansour et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

The Evaluation of Arthrospira platensis Bioactivity and their Dietary Supplementation to Nile Tilapia Vegetarian Diet on Growth Performance, Feed Utilization, Body Composition and Hemato-Biochemical Parameters

Published Online: Aug 05, 2021

Page range: 1061 - 1080

Received: Oct 08, 2020

Accepted: Jan 18, 2021

DOI: https://doi.org/10.2478/aoas-2021-0003

Keywordscarotenoids identification, DPPH assay, growth performance, physiological response, Oreochromis niloticus

© 2021 Abdallah Tageldein Mansour et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
carotenoids identification, DPPH assay, growth performance, physiological response, Oreochromis niloticus