<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Cystic endometrial hyperplasia-pyometra complex (CEH/P) significantly perturbs the reproductive performance of affected bitches and ovariohysterectomy (OHE) is a commonly applied treatment. Thus the only way to take advantage of the genetic potential of valuable females is application of assisted reproductive techniques (ART) mainly <italic>in vitro</italic> embryo production (IVP) or in some exceptional cases animal cloning by somatic cell nuclear transfer (SCNT). The aim of our study was to examine a potential effect of the CEH/P status on the quality of oocytes from females subjected to OHE. In total, 828 immature oocytes collected from ovaries of 33 bitches (21 control, 12 CEH/P) were subjected to genetic analyses (mRNA expression of two maternal-effect genes: <italic>GDF-9, OCT4</italic> and mitochondrial DNA (mtDNA) content). Oocytes of CEH/P females were characterized by a higher mtDNA content (471 696) than gametes of their healthy counterparts (368 175; P&lt;0.005). Transcripts for the two genes were detected in all samples and the mRNA level was not affected by the CEH/P status. In conclusion, the CEH/P complex does not exert a negative effect on oocyte quality reflected by the two parameters examined in this study.</p></abstract>

Cystic endometrial hyperplasia-pyometra complex (CEH/P) significantly perturbs the reproductive performance of affected bitches and ovariohysterectomy (OHE) is a commonly applied treatment. Thus the only way to take advantage of the genetic potential of valuable females is application of assisted reproductive techniques (ART) mainly in vitro embryo production (IVP) or in some exceptional cases animal cloning by somatic cell nuclear transfer (SCNT). The aim of our study was to examine a potential effect of the CEH/P status on the quality of oocytes from females subjected to OHE. In total, 828 immature oocytes collected from ovaries of 33 bitches (21 control, 12 CEH/P) were subjected to genetic analyses (mRNA expression of two maternal-effect genes: GDF-9, OCT4 and mitochondrial DNA (mtDNA) content). Oocytes of CEH/P females were characterized by a higher mtDNA content (471 696) than gametes of their healthy counterparts (368 175; P<0.005). Transcripts for the two genes were detected in all samples and the mRNA level was not affected by the CEH/P status. In conclusion, the CEH/P complex does not exert a negative effect on oocyte quality reflected by the two parameters examined in this study.

Pyometra does not affect some molecular quality-related parameters of canine oocytes

Department of Genetics and Animal Breeding

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cystic endometrial hyperplasia-pyometra complex (CEH/P) significantly perturbs the reproductive performance of affected bitches and ovariohysterectomy (OHE) is...

Pyometra does not affect some molecular quality-related parameters of canine oocytes

Published Online: Jan 29, 2021

Page range: 193 - 204

Received: Jan 08, 2020

Accepted: Jun 03, 2020

DOI: https://doi.org/10.2478/aoas-2020-0062

Keywords
CEH/P, domestic dog, immature oocyte, maternal-effect genes, mtDNA

© 2020 Piotr Pawlak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Pyometra does not affect some molecular quality-related parameters of canine oocytes

Published Online: Jan 29, 2021

Page range: 193 - 204

Received: Jan 08, 2020

Accepted: Jun 03, 2020

DOI: https://doi.org/10.2478/aoas-2020-0062

KeywordsCEH/P, domestic dog, immature oocyte, maternal-effect genes, mtDNA

© 2020 Piotr Pawlak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
CEH/P, domestic dog, immature oocyte, maternal-effect genes, mtDNA