<abstract xmlns="http://www.w3.org/1999/xhtml">It was hypothesized that dietary copper (Cu) nanoparticles, as a substitute for the commonly used copper sulfate, could contribute to lowering the dietary inclusion levels of Cu without compromising growth performance or reducing Cu digestibility and utilization in turkeys. An experiment was carried out on 648 one-day-old Hybrid Converter turkeys divided into 6 groups with 6 replicates per group in a two-factorial design with 3 dietary inclusion levels of Cu (20, 10 and 2 mg kg−1) and 2 dietary sources of Cu, copper sulfate and Cu nanoparticles (Cu-SUL and Cu-NPs, respectively). The apparent digestibility coefficients of minerals were determined after 6 weeks, and tissue samples were collected after 14 weeks of experimental feeding. A decrease in the dietary inclusion levels of Cu from 20 to 10 and 2 mg kg−1 did not reduce the body weights of turkeys at 42 and 98 days of age. In comparison with the remaining treatments, the lowest dietary inclusion level of Cu significantly decreased MDA concentrations in small intestinal tissue (P=0.002) and in the bursa of Fabricius (P=0.001). The replacement of Cu-SUL with Cu-NPs differentially modulated the redox status of selected tissues, i.e., enhanced SOD activity in small intestinal tissue (P=0.001) and decreased total glutathione levels in the bursa of Fabricius (P=0.005). In general, neither the different levels nor sources of additional dietary Cu (main factors) exerted negative effects on the histological structure of the duodenum and jejunum in turkeys. The intestinal digestibility of Cu increased with decreasing dietary Cu levels, and as a consequence, the highest apparent digestibility coefficient of Cu (and zinc) was noted in turkeys fed diets with the addition of 2 mg kg−1 Cu-NPs. Therefore, the environmental burden of excreted Cu was substantially reduced along with decreasing dietary Cu levels but it did not depend on the Cu source.</abstract>

It was hypothesized that dietary copper (Cu) nanoparticles, as a substitute for the commonly used copper sulfate, could contribute to lowering the dietary inclusion levels of Cu without compromising growth performance or reducing Cu digestibility and utilization in turkeys. An experiment was carried out on 648 one-day-old Hybrid Converter turkeys divided into 6 groups with 6 replicates per group in a two-factorial design with 3 dietary inclusion levels of Cu (20, 10 and 2 mg kg−1) and 2 dietary sources of Cu, copper sulfate and Cu nanoparticles (Cu-SUL and Cu-NPs, respectively). The apparent digestibility coefficients of minerals were determined after 6 weeks, and tissue samples were collected after 14 weeks of experimental feeding. A decrease in the dietary inclusion levels of Cu from 20 to 10 and 2 mg kg−1 did not reduce the body weights of turkeys at 42 and 98 days of age. In comparison with the remaining treatments, the lowest dietary inclusion level of Cu significantly decreased MDA concentrations in small intestinal tissue (P=0.002) and in the bursa of Fabricius (P=0.001). The replacement of Cu-SUL with Cu-NPs differentially modulated the redox status of selected tissues, i.e., enhanced SOD activity in small intestinal tissue (P=0.001) and decreased total glutathione levels in the bursa of Fabricius (P=0.005). In general, neither the different levels nor sources of additional dietary Cu (main factors) exerted negative effects on the histological structure of the duodenum and jejunum in turkeys. The intestinal digestibility of Cu increased with decreasing dietary Cu levels, and as a consequence, the highest apparent digestibility coefficient of Cu (and zinc) was noted in turkeys fed diets with the addition of 2 mg kg−1 Cu-NPs. Therefore, the environmental burden of excreted Cu was substantially reduced along with decreasing dietary Cu levels but it did not depend on the Cu source.

Effect of Different Levels of Copper Nanoparticles and Copper Sulfate on Morphometric Indices, Antioxidant Status and Mineral Digestibility in the Small Intestine of Turkeys

Department of Poultry Science, Faculty of Animal Bioengineering

University of Warmia and Mazury in Olsztyn

Department of Large Animal Diseases with Clinic

Department of Biochemistry and Toxicology, Faculty of Biology, Animal Sciences and Bioeconomy

Institute of Animal Reproduction and Food Research

Department of Animal Nutrition and Biotechnology

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

It was hypothesized that dietary copper (Cu) nanoparticles, as a substitute for the commonly used copper sulfate, could contribute to lowering the dietary...

Effect of Different Levels of Copper Nanoparticles and Copper Sulfate on Morphometric Indices, Antioxidant Status and Mineral Digestibility in the Small Intestine of Turkeys

Published Online: Aug 01, 2020

Page range: 975 - 990

Received: Aug 27, 2019

Accepted: Jan 09, 2020

DOI: https://doi.org/10.2478/aoas-2020-0013

Keywords
copper, nanoparticles, small intestine, redox status, turkeys

© 2020 Jan Jankowski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of Different Levels of Copper Nanoparticles and Copper Sulfate on Morphometric Indices, Antioxidant Status and Mineral Digestibility in the Small Intestine of Turkeys

Published Online: Aug 01, 2020

Page range: 975 - 990

Received: Aug 27, 2019

Accepted: Jan 09, 2020

DOI: https://doi.org/10.2478/aoas-2020-0013

Keywordscopper, nanoparticles, small intestine, redox status, turkeys

© 2020 Jan Jankowski et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
copper, nanoparticles, small intestine, redox status, turkeys