1. bookVolume 19 (2019): Issue 3 (July 2019)
Journal Details
License
Format
Journal
eISSN
2300-8733
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
access type Open Access

Dietary Biotechnological Ajuga reptans Extract in Post Weaning Piglets: Effects on Growth Performance, Oxidative Status and Immune Parameters

Published Online: 30 Jul 2019
Volume & Issue: Volume 19 (2019) - Issue 3 (July 2019)
Page range: 793 - 806
Received: 21 Feb 2019
Accepted: 04 Apr 2019
Journal Details
License
Format
Journal
eISSN
2300-8733
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
Abstract

The effect of dietary supplementation with a biotechnological extract of Ajuga reptans on growth performance, oxidative status and immune parameters was evaluated in post weaning piglets. At weaning, 120 piglets with an average live weight of 8.1±1.3 kg, were assigned to one of three experimental groups. The first group was fed a control diet (C). The second and third groups were fed the same diet supplemented with 5 mg (T1) and 10 mg (T2) of teupolioside/kg feed from a biotechnological plant extract. Growth performances were recorded and blood samples were collected at the beginning, at 14 days, and at the end of the trial (56 days). Serum biochemical parameters, oxidative status and immunoglobulin titres were determined. Average daily gain tended to be higher (P=0.057) and live weight was higher in piglets (P<0.05) fed with different amounts of plant extract (T1 and T2) than the controls. The production of reactive oxygen metabolites (ROMs) was higher (P<0.05) in the control group than in the groups receiving teupolioside (T1 and T2). Concentration of serum immunoglobulin of class G improved (P<0.001) in piglets fed the T1 and T2 diets than the controls. Overall, the results suggested that the biotechnological extract of Ajuga reptans containing teupolioside has an antioxidant and immunomodulant effect.

Keywords

Alberti A., Bolognini L., Macciantelli D., Caratelli M. (2000). The radical cation of N, N-diethyl-para-phenylendiamine: a possible indicator of oxidative stress in biological samples. Res. Chem. Intermediat., 26: 253–267.Search in Google Scholar

Brüssow H. (2017). Adjuncts and alternatives in the time of antibiotic resistance and in-feed antibiotic bans. Microb. Biotechnol., 10: 674–677.Search in Google Scholar

Buchet A., Belloc C., Leblanc-Maridor M., Merlot E. (2017). Effects of age and weaning conditions on blood indicators of oxidative status in pigs. PLoS One, 12: e0178487.Search in Google Scholar

Casamassima D., Palazzo M., Presutti T., Colella G.E. (2009). Productive performances, plasmatic oxidative status and some blood parameters in suckling lambs supplemented with verbascoside. It. J. Anim. Sci., 8: 668.Search in Google Scholar

Casamassima D., Palazzo M., Martemucci G., Vizzarri F., Corino C. (2012). Effects of verbascoside on plasma oxidative status and blood and milk production parameters during the peripartum period in Lacaune ewes. Small Rumin. Res., 105: 1–8.Search in Google Scholar

Casamassima D., Palazzo M., Vizzarri F., Cinone M., Corino C. (2013). Effect of dietary phenylpropanoid glycoside-based natural extracts on blood parameters and productive performance in intensively-reared young hares. Czech J. Anim. Sci., 58: 270–278.Search in Google Scholar

Casamassima D., Nardoia M., Palazzo M., Vizzarri F., D ’ Alessandro A.G., Corino C. (2014). Effect of dietary extruded linseed, verbascoside and vitamin E supplements on yield and quality of milk in Lacaune ewes. J. Dairy Res., 81: 485–493.Search in Google Scholar

Cesarone M.R., Belcaro G., Caratelli M., Cornelli U., De Sanctis M.T., Incandela L., Barsott R., Terranova R., Nicolaides A. (1999). A simple test to monitor oxidative stress. Intern. Angiol., 2: 127–130.Search in Google Scholar

Cheng G., Hao H., Xie S., Xu W., Dai M., Huang L., Yuan Z. (2014). Antibiotic alternatives: the substitution of antibiotics in animal husbandry? Front. Microbiol., 5: 217.Search in Google Scholar

Cuevas A., Saavedra N., Salazar L.A., Abdalla D.S.P. (2013). Modulation of immune function by polyphenols: possible contribution of epigenetic factors. Nutrients, 5: 2314–2332.Search in Google Scholar

Dal Toso R., Melandri F. (2009). Biotechnologically produced botanical ingredients Ajuga reptans extract titrated in teupolioside. Nutrafoods, 8: 29–36.Search in Google Scholar

D’ Alessandro A.G., Vizzarri F., Palazzo M., Martemucci G. (2017). Dietary verbascoside supplementation in donkeys: effects on milk fatty acid profile during lactation, and serum biochemical parameters and oxidative markers. Animal, 11: 1505–1512.Search in Google Scholar

De Marco M., Salcedo W.L., Pastorelli G., Rossi R., Corino C., Bergagna S., Mellia E., Gennero M.S., Biasibetti E., Capucchio M.T., Nurisso S., Tarantola M., Forneris G., Schiavone A. (2015). Effects of verbascoside supplemented diets on growth performance, blood traits, meat quality, lipid oxidation and histological features in broiler chickens. It. J. Anim. Sci., 14: 172–178.Search in Google Scholar

Dembitsky V.M. (2005). Astonishing diversity of natural surfactants: 5. Biologically active glycosides of aromatic metabolites. Lipids, 40: 869–900.Search in Google Scholar

Devi S.M., Lee S.I., Kim I.H. (2015). Effect of phytogenics on growth performance, fecal score, blood profiles, fecal noxious gas emission, digestibility, and intestinal morphology of weanling pigs challenged with Escherichia coli K88. Pol. J. Vet. Sci., 18: 557–564.Search in Google Scholar

Di Giancamillo A., Rossi R., Pastorelli G., Deponti D., Carollo V., Casamassima D., Domeneghini C., Corino C. (2015). The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets. J. Anim. Sci., 93: 284–959.Search in Google Scholar

Di Paola R., Esposito E., Mazzon E., Riccardi L., Caminiti R., Dal Toso R., Pressi G., Cuzzocrea S. (2009). Teupolioside, a phenylpropanoid glycosides of Ajuga reptans, biotechnologically produced by IRB plant cell line, exerts beneficial effects on a rodent model of colitis. Biochem. Pharmacol., 77: 845–857.Search in Google Scholar

European Surveillance of Veterinary Antimicrobial Consumption (2016). Sales of veterinary antimicrobial agents in 29 European countries in 2014. Trends from 2011 to 2014. VI ESVAC report. 14.10. 2016.Search in Google Scholar

European Patent EP 1 997 501 A2. Extracts from Ajuga reptans cell lines, their preparation and use. Date of publication: 03.12.2008 Bulletin 2008/49.Search in Google Scholar

Food and Drug Administration (2013). Animal Drug User Fee Act (ADUFA) public meeting. Food and Drug Administration Center for Veterinarian Medicine.18.12 2012.Search in Google Scholar

Gheisar M.M., Kim M. (2018). Phytobiotics in poultry and swine nutrition – a review. It. Anim. Sci., 17: 92–99.Search in Google Scholar

Gresse R., Chaucheyras-Durand F., Fleury M.A., Vande Wiele T., Forano E., Blanquet-Diot S. (2017). Gut microbiota dysbiosis in postweaning piglets: understanding the keys to health. Trends Microbiol., 25: 851–873.Search in Google Scholar

Hassanain E., Silverberg J.I., Norowitz K.B., Chice S., Bluth M.H., Brody N., Joks R., Durkin H.G., Smith-Norowitz T.A. (2010). Green tea (Camelia sinensis) suppresses B cell production of IgE without inducing apoptosis. Ann. Clin. & Lab. Sci., 40: 135–143.Search in Google Scholar

Johnson R., McNutt P., Mac Mahon S., Robson R. (1997). Use of the Friedewald formula to estimate LDL-cholesterol in patients with chronic renal failure on dialysis. Clin. Chem., 11: 2183–2184.Search in Google Scholar

Kamel C. (2001). Tracing modes of action and the roles of plant extracts in non-ruminants. In: Recent Advances in Animal Nutrition, Garnsworthy P.C., Wiseman J. (eds), pp. 135–150.Search in Google Scholar

Kanitz E., Tuchscherer M., Puppe B., Tuchscherer A., Stabenow B. (2004). Consequences of repeated early isolation in domestic piglets (Sus scrofa) on their behavioural, neuroendocrine, and immunological responses. Brain Behav. Immun., 18: 35–45.Search in Google Scholar

Korkina L.G., Mikhalchik E., Suprun M.V., Pastore S., Dal Toso R. (2006). Molecular mechanisms underlying wound healing and anti-inflammatory properties of naturally occurring biotechnologically produced phenylpropanoid glycosides. Cell. Mol. Biol., 53: 78–83.Search in Google Scholar

Landete J.M. (2012). Updated knowledge about polyphenols: functions, bioavailability, metabolism, and health. Crit. Rev. Food Sci. Nutr., 52: 936–948.Search in Google Scholar

Le Dividich J., Sève B. (2000). Effects of under feeding during the weaning period on growth, metabolism, and hormonal adjustments in the piglet. Domestic Anim. Endocrinol., 19: 63–74.Search in Google Scholar

Lipiński K., Mazur M., Antoszkiewicz Z., Purwin C. (2017). Polyphenols in monogastric nutrition – a review. Ann. Anim. Sci., 17: 41–58.Search in Google Scholar

Looft T., Johnson T.A., Allen K.H., Bayles D.O., Alt D.P., Stedtfeld R.D., Sul W.J., Stedtfeld T.M., Chai B., Cole J.R., Hashsham S.A., Tiedje J.M., Stanton T.B. (2012). In-feed antibiotic effects on the swine intestinal microbiome. Proc. Natl. Acad. Sci., 109: 1691–1696.Search in Google Scholar

Lykkesfeldt J., Svendsen O. (2007). Oxidants and antioxidants in disease: Oxidative stress in farm animals. Vet. J., 173: 502–511.Search in Google Scholar

Maass N., Bauer J., Paulicks B.R., Böhmer B.M., Roth-Maier D.A. (2005). Efficiency of Echinacea purpurea on performance and immune status in pigs. J. Anim. Physiol. Anim. Nutr., 89: 244–252.Search in Google Scholar

Maghin F., Rossi R., Prost M., Corino C. (2016). Biological system to assess the antioxidant capability of plant extracts. Proc. Nutriox meeting. Kaiserslautern, Germany, 21-23.09.2016.Search in Google Scholar

Montagne L., Boudry G., Favier C., Le Huërou-Luron I., Lallès J.P., Sève B. (2007). Main intestinal markers associated with the changes in gut architecture and function in piglets after weaning. Br. J. Nutr., 97: 45–57.Search in Google Scholar

National Research Council (1998). Nutrient Requirements of Swine. 10th rev. eds, National Academy Press: Washington, DC.Search in Google Scholar

Palazzo M., Vizzarri F., Cinone M., Corino C., Casamassima D. (2011). Assessment of a natural dietary extract, titrated in phenylpropanoid glycosides, on blood parameters and plasma oxidative status in intensively reared Italian hares (Lepus corsicanus). Animal, 5: 844–850.Search in Google Scholar

Pastore S., Potapovich A., Kostyuk V., Mariani V., Lulli D., De Luca C., Korkina L. (2009). Plant polyphenols effectively protect HaCaT cells from ultraviolet C-triggered necrosis and suppress inflammatory chemokine expression. Ann. New York Acad. Sci., 1171: 305–313.Search in Google Scholar

Pastorelli G., Rossi R., Corino C. (2012). Influence of Lippia citriodora verbascoside on growth performance, antioxidant status, and serum immunoglobulins content in piglets. Czech J. Anim. Sci., 57: 312–322.Search in Google Scholar

Paszkiewicz M., Budzynska A., Rozalska B., Sadowska B. (2012). The immunomodulatory role of plant polyphenols (in Polish). Postep. Hig. Med. Dosw., 66: 637–646.Search in Google Scholar

Prost M. (1992). Process for the determination by means of free radicals of the antioxidant properties of a living organism or potentially aggressive agents. In: US Patent 5, 135, 850.Search in Google Scholar

Rossi R., Pastorelli G., Cannata S., Corino C. (2009). Effect of weaning on total antiradical activity in piglets. Proc. of the XVIII Animal Science and Production Association Congress, Palermo, Italy 9-12.06.2009, p. 673.Search in Google Scholar

Rossi R., Pastorelli G., Corino C. (2013). Application of KRL test to assess total antioxidant activity in pigs: sensitivity to dietary antioxidants. Res. Vet. Sci., 94: 372–377.Search in Google Scholar

Rossi R., Ratti S., Pastorelli G., Maghin F., Martemucci G., Casamassima D., D ’ Alessandro A.G., Corino C. (2017). Effect of dietary plant extract on meat quality and sensory parameters of meat from Equidae. J. Sci. Food Agric., 97: 4690–4696.Search in Google Scholar

Sanbongi C., Suzuki N., Sakane T. (1997). Polyphenols in chocolate, which have antioxidant activity, modulate immune functions in humans in vitro. Cell. Immunol., 177: 129–136.Search in Google Scholar

Sangwan N.S., Farooqi A.H.A., Shabih F., Sangwan R.S. (2001). Regulation of essential oil production in plants. Plant Growth Reg., 34: 3–21.Search in Google Scholar

Sauerwein H., Schmitz S., Hiss S. (2007). Effects of a dietary application of a yeast cell wall extract on innate and acquired immunity, on oxidative status and growth performance in weanling piglets and on the ileal epithelium in fattened pigs. J. Anim. Physiol. Anim. Nutr., 91: 369–380.Search in Google Scholar

Van Boeckel T.P., Brower C., Gilbert M., Grenfell B.T., Levin S.A., Robinson T.P., Teillant A., Laxminarayan R. (2015). Global trends in antimicrobial use in food animals. Proc. Nat. Acad. Sci., 112: 5649–5654.Search in Google Scholar

Vizzarri F., Nardoia M., Palazzo M. (2014). Effect of dietary Lippia citriodora extract on productive performance and meat quality parameters in hares (Lepus europaeus Pall.). Archiv. Tierzucht, 57: 1–7.Search in Google Scholar

World Health Organization (WHO) (2017). Stop using antibiotics in healthy animals to prevent the spread of antibiotic resistance, 07 November, Geneve.Search in Google Scholar

Zunino S.J., Storms D.H. (2009). Resveratrol alters proliferative responses and apoptosis in human activated B lymphocytes in vitro. J. Nutr., 139: 1603–1608.Search in Google Scholar

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