1. bookVolume 13 (2013): Issue 3 (July 2013)
Journal Details
License
Format
Journal
eISSN
2300-8733
ISSN
1642-3402
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
access type Open Access

Assessment of in Vitro Developmental Capacity of Porcine Nuclear-Transferred Embryos Reconstituted with Cumulus Oophorus Cells Undergoing Vital Diagnostics for Apoptosis Detection / Ocena zdolności rozwojowych in vitro klonalnych zarodków świni rekonstytuowanych z jąder komórek wzgórka jajonośnego poddawanych przyżyciowej diagnostyce w kierunku wykrywania apoptozy

Published Online: 01 Aug 2013
Volume & Issue: Volume 13 (2013) - Issue 3 (July 2013)
Page range: 513 - 529
Journal Details
License
Format
Journal
eISSN
2300-8733
ISSN
1642-3402
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
Abstract

The objective of the current investigation was to extensively compare the in vitro developmental capabilities between cloned pig embryos reconstructed with the cell nuclei of either cumulus oophorus cells or adult dermal fibroblast cells that were both evaluated as non-apoptotic on the basis of YO-PRO-1- and Annexin V-eGFP-mediated vital analysis for programmed cell suicide. In Group I, the competences of nuclear-transferred (NT) embryos that were derived from non-apoptotic/ non-necrotic (i.e., YO-PRO-1- and Annexin V-eGFP-negative) cumulus cells to complete their development to the morula and blastocyst stages were maintained at the proportions of 155/364 (42.6%) and 54/364 (14.8%), respectively. In Group II, NT embryos that were reconstituted with non-apoptotic and/or non-necrotic adult cutaneous fibroblast cells developed to the morula and blastocyst stages at the rates of 207/358 (57.8%) and 110/358 (30.7%), respectively. Although the in vitro developmental potential of porcine NT embryos derived from non-apoptotic/non-necrotic cumulus cells was significantly lower (P<0.001) than that of NT embryos reconstructed with adult dermal fibroblast cells, the obtained morula/blastocyst formation rates turned out to be considerably higher as compared to the rates reported by other investigators. Altogether, to our knowledge, the comprehensive research aimed at the determination of preimplantation developmental outcomes of cloned pig embryos produced using nuclear donor somatic cells of different provenance (cumulus oophorus cells or adult cutaneous fibroblast cells) that were vitally diagnosed for the lack of proapoptotic transformations in their plasma membranes has not yet been accomplished.

Keywords

Anger M., Kues W.A., Klima J., Mielenz M., Kubelka M., Motlik J., Esner M., Dvorak P., Carnwarth J.W., Niemann H. (2003). Cell cycle dependent expression of Plk1 in synchronized porcine fetal fibroblasts. Mol. Reprod. Dev., 65: 245-253.1278424510.1002/mrd.10289Search in Google Scholar

Bondioli K., Ramsoondar J., Williams B., Costa C., Fodor W. (2001). Cloned pigs generated from cultured skin fibroblasts derived froma H-transferase transgenic boar. Mol. Reprod. Dev., 60: 189-195.10.1002/mrd.1076Open DOISearch in Google Scholar

Boquest A.C., Grupen C.G., Harrison S.J., Mc Ilfatrick S.M., Ashman R.J.,d’ Apice A.J.F., Nottle M.B. (2002). Production of cloned pigs from cultured fetal fibroblast cells. Biol. Reprod., 66: 1283-1287.10.1095/biolreprod66.5.128311967188Open DOISearch in Google Scholar

Cho J.K., Lee B.C., Park J.I., Lim J.M., Shin S.J., Kim K.Y., Lee B.D., Hwang W.S. (2002). Development of bovine oocytes reconstructed with different donor somatic cells with or without serum starvation. Theriogenology, 57: 1819-1828.1204168610.1016/S0093-691X(01)00699-9Search in Google Scholar

Daly J.M., Jannot C.B., Beerli R.R., Graus- Porta D., Maurer F.G., Hynes N.E. (1997). New differentiation factor induces Erb B2 down-regulation and apoptosis of Erb B2-overexpressing breast tumor cells. Cancer Res., 57: 3804-3811.Search in Google Scholar

De Sousa P., Dobrinsky J.R., Zhu J., Archibald A.L., Ainslie A., Bosma W., Bowering J., Bracken J., Ferrier P.M., Fletcher J., Gasparrini B., Hark -ness L., Johnston P., Ritchie M., Ritchie W.A., Travers A., Albertini D., Din-nyes A., King T.J., Wilmut I. (2002). Somatic cell nuclear transfer in the pig: control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy. Biol. Reprod., 66: 642-650.10.1095/biolreprod66.3.64211870070Open DOISearch in Google Scholar

Engeland M.,vanden Eijnde S.M., Aken T., Vermeij- Keers C., Ramaekers F.C., Schutte B., Reutelingsperger C.P. (2001). Detection of apoptosis in ovarian cells in vitro and in vivo using the annexin V-affinity assay. Methods Mol. Med., 39: 669-677.Search in Google Scholar

Estaquier J., Idziorek T., Zou W., Emilie D., Farber C.M., Bourez J.M., Amei -sen J.C. (1995). Thelper type 1/Thelper type 2 cytokines and Tcell death: preventive effect of interleukin 12 on activation-induced and CD95 (FAS/APO-1)-mediated apoptosis of CD4+ cells from human immunodeficiency virus-infected persons. J. Exp. Med., 182: 1759-1767.Search in Google Scholar

Estaquier J., Taneka M., Suda T., Nagata S., Golstein P., Ameisen J.C. (1996). Fas-mediated apoptosis of CD4+ and CD8+ Tcells from human immunodeficiency virus-infected persons: differential in vitro preventive effect of cytokines and protease antagonists. Blood, 87: 4959-4966.Search in Google Scholar

Fadok V.A., Savill J.S., Haslett D.L., Bratton G.L., Doherty P.A., Campbell P.A., Henson P.M. (1992 a). Different populations of macrophages use either the vitronectin receptor or the phosphatidylserine receptor to recognize and remove apoptotic cells. J. Immunol., 149: 4029-4035.Search in Google Scholar

Fadok V.A., Voelker D.R., Campbell P.A., Cohen J.J., Bratton G.L., Henson P.M. (1992 b). Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J. Immunol., 148: 2207-2216.Search in Google Scholar

Fadok V.A., Lazlo D.J., Noble P.W., Weinstein L., Riches D.W.H., Henson P.M. (1993). Particle digestibility is required for induction of the phosphatidylserine recognition mechanism used by murine macrophages to phagocytose apoptotic cells. J. Immunol., 151: 4274-4285.Search in Google Scholar

Frasch S.C., Henson P.M., Nagaosa K., Fessler M.B., Borregaard N., Bratton D.L. (2004). Phospholipid flip-flop and phospholipid scramblase 1 (PLSCR1) co-localize to uropod rafts in formylated Met-Leu-Phe-stimulated neutrophils. J. Biol. Chem., 279: 17625-17633.Search in Google Scholar

Hyun S., Lee G., Kim D., Kim H., Lee S., Nam D., Jeong Y., Kim S., Yeom S., Kang S., Han J., Lee B., Hwang W. (2003). Production of nuclear transfer-derived piglets using porcine fetal fibroblasts transfected with the enhanced green fluorescent protein. Biol. Reprod., 69: 1060-1068.10.1095/biolreprod.102.0148861277342912773429Open DOISearch in Google Scholar

Idziorek T., Estaquier J., De Bels F., Ameisen J.C. (1995). YO-PRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J. Immunol. Methods, 185: 249-258.Search in Google Scholar

Jang G., Park E.S., Cho J.K., Bhuiyan M.M., Lee B.C., Kang S.K., Hwang W.S. (2004). Preimplantational embryo development and incidence of blastomere apoptosis in bovine somatic cell nuclear transfer embryos reconstructed with long-term cultured donor cells. Theriogenology, 62: 512-521.10.1016/j.theriogenology.2003.11.0221522600715226007Open DOISearch in Google Scholar

Jiang L., Carter D.B., Xu J., Yang X., Prather R.S., Tian X.C. (2004). Telomere lengths in cloned transgenic pigs. Biol. Reprod., 70: 1589-1593.10.1095/biolreprod.103.0226161474929614749296Open DOISearch in Google Scholar

Koo D.-B., Kang Y.-K., Park J.S., Park J.-K., Chang W.-K., Lee K.-K., Han Y.-M. (2004). Apaucity of structural integrity in cloned porcine blastocysts produced in vitro. Theriogenology, 62: 779-789.10.1016/j.theriogenology.2003.12.02715251229Open DOISearch in Google Scholar

Kues W.A., Carnwarth J.W., Paul D., Niemann H. (2002). Cell cycle synchronization of porcine fetal fibroblasts by serum deprivation initiatesanonconventional form of apoptosis. Cloning Stem Cells, 4: 231-243.1239880410.1089/1536230026033951112398804Search in Google Scholar

Lee J.-W., Wu S.-C., Tian X.C., Barber M., Hoagland T., Riesen J., Lee K.-H., Tu C.-F., Cheng W.T.K., Yang X. (2003 a). Production of cloned pigs by whole-cell intracytoplasmic microinjection. Biol. Reprod., 69: 995-1001.1277341810.1095/biolreprod.103.015917Search in Google Scholar

Lee G.S., Hyun S.H., Kim H.S., Kim D.Y., Lee S.H., Lim J.M., Lee E.S., Kang S.K., Lee B.C., Hwang W.S. (2003 b). Improvement ofaporcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations. Theriogenology, 59: 1949-1957.1260073210.1016/S0093-691X(02)01294-3Search in Google Scholar

Lee G.S., Kim H.S., Hyun S.H., Lee S.H., Jeon H.Y., Nam D.H., Jeong Y.W., Kim S., Kim J.H., Han J.Y., Ahn C., Kang S.K., Lee B.C., Hwang W.S. (2005). Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent protein. Theriogenology, 63: 973-991.1571018610.1016/j.theriogenology.2004.04.01715710186Search in Google Scholar

Lincz L.F. (1998). Deciphering the apoptotic pathway: all roads lead to death. Immunol. Cell Biol., 76: 1-19.955377110.1046/j.1440-1711.1998.00712.x9553771Search in Google Scholar

Martin S.J., Reutelingsperger C.P., Mc Gahon A.J., Rader J.A.,van Schie R.C., La Face D.M., Green D.R. (1995). Early redistribution of plasma membrane phosphatidylserine isageneral feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J. Exp. Med., 182: 1545-1556.10.1084/jem.182.5.154521921827595224Open DOISearch in Google Scholar

Park K.-W., Lai L., Cheong H.-T., Cabot R., Sun Q.-Y., Wu G., Rucker E.B., Durt -schi D., Bonk A., Samuel M., Rieke A., Day B.N., Murphy C.N., Carter D.B., Prather R.S. (2002). Mosaic gene expression in nuclear transfer-derived embryos and the production of cloned transgenic pigs from ear-derived fibroblasts. Biol. Reprod., 66: 1001-1005.10.1095/biolreprod66.4.100111906919Open DOISearch in Google Scholar

Ramsoondar J.J., Machaty Z., Costa C., Williams B.L., Fodor W.L., Bondioli K.R. (2003). Production of α1,3-galactosyltransferase-knockout cloned pigs expressing human α1,2- fucosylosyltransferase. Biol. Reprod., 69: 437-445.Search in Google Scholar

Samiec M. (2004). Development of pig cloning studies: past, present and future. J. Anim. Feed Sci., 13: 211-238.Search in Google Scholar

Samiec M. (2005 a). The effect of mitochondrial genome on architectural remodeling and epigenetic reprogramming of donor cell nuclei in mammalian nuclear transfer-derived embryos. J. Anim. Feed Sci., 14: 393-422.10.22358/jafs/67034/2005Search in Google Scholar

Samiec M. (2005 b). The role of mitochondrial genome (mt DNA) in somatic and embryo cloning of mammals. J. Anim. Feed Sci., 14: 213-233.10.22358/jafs/67008/2005Search in Google Scholar

Samiec M., Skrzyszowska M. (2005 a). Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the method and its technical advantages:areview. Czech J. Anim. Sci., 50: 235-242.10.17221/4163-CJASSearch in Google Scholar

Samiec M., Skrzyszowska M. (2005 b). Molecular conditions of the cell nucleus remodelling/ reprogramming process and nuclear-transferred embryo development in the intraooplasmic karyoplast injection technique:areview. Czech J. Anim. Sci., 50: 185-195.10.17221/4142-CJASSearch in Google Scholar

Samiec M., Skrzyszowska M. (2010 a). The use of different methods of oocyte activation for generation of porcine fibroblast cell nuclear-transferred embryos. Ann. Anim. Sci., 10: 399-411.Search in Google Scholar

Samiec M., Skrzyszowska M. (2010 b). Preimplantation developmental capability of cloned pig embryos derived from different types of nuclear donor somatic cells. Ann. Anim. Sci., 10: 385-398.Search in Google Scholar

Samiec M., Skrzyszowska M. (2011 a). The possibilities of practical application of transgenic mammalian species generated by somatic cell cloning in pharmacology, veterinary medicine and xenotransplantology. Pol. J. Vet. Sci., 14: 329-340.10.2478/v10181-011-0051-6Search in Google Scholar

Samiec M., Skrzyszowska M. (2011 b). Transgenic mammalian species, generated by somatic cell cloning, in biomedicine, biopharmaceutical industry and human nutrition/dietetics - recent achievements. Pol. J. Vet. Sci., 14: 317-328.10.2478/v10181-011-0050-7Search in Google Scholar

Samiec M., Skrzyszowska M. (2012 a). Roscovitine isanovel agent that can be used for the activation of porcine oocytes reconstructed with adult cutaneous or fetal fibroblast cell nuclei. Theriogenology, 78: 1855-1867.2297996310.1016/j.theriogenology.2012.06.029Search in Google Scholar

Samiec M., Skrzyszowska M. (2012 b). High developmental capability of porcine cloned embryos following trichostatin A-dependent epigenomic transformation during in vitro maturation of oocytes pre-exposed to R-roscovitine. Anim. Sci. Pap. Rep., 30: 383-393.Search in Google Scholar

Samiec M., Skrzyszowska M., Lipi ński D. (2012). Pseudophysiological transcomplementary activation of reconstructed oocytes asahighly efficient method used for producing nucleartransferred pig embryos originating from transgenic foetal fibroblast cells. Pol. J. Vet. Sci., 15: 509-516.Search in Google Scholar

Samiec M., Skrzyszowska M., Smor ąg Z. (2003). Effect of activation treatments on the in vitro developmental potential of porcine nuclear transfer embryos. Czech J. Anim. Sci., 48: 499-507.Search in Google Scholar

Schuetz A.W., Whittingham D.G., Snowden R. (1996). Alterations in the cell cycle of mouse cumulus granulosa cells during expansion and mucification in vivo and in vitro. Reprod. Fertil. Dev., 8: 935-943.Search in Google Scholar

Skrzyszowska M., Samiec M. (2005). Production of porcine nuclear transfer embryos using fetal fibroblast cells analyzed on apoptosis. Reprod. Fertil. Dev., 17: 182-183.Search in Google Scholar

Skrzyszowska M., Samiec M. (2008). Live plasma membrane analysis of early apoptotic cell death in porcine adult dermal fibroblasts prior to somatic cell cloning. Reprod. Fertil. Dev., 20, p. 108.10.1071/RDv20n1Ab55Search in Google Scholar

Skrzyszowska M., Karasiewicz J., Bednarczyk M., Samiec M., Smor ąg Z., Wa ś B., Guszkiewicz A., Korwin - Kossakowski M., G órniewska M., Szab -listy E., Modliński J.A., Łakota P., Wawrzy ńska M., Sechman A., Wojty-siak D., Hrabia A., Mika M., Lisowski M., Czekalski P., Rz ąsa J., Kapkow -ska E. (2006). Generation of cloned and chimeric embryos/offspring using the new methods of animal biotechnology. Reprod. Biol., 6 (Suppl. 1): 119-135.Search in Google Scholar

Skrzyszowska M., Samiec M., S łomski R., Lipi ński D., Ma ły E. (2008). Development of porcine transgenic nuclear-transferred embryos derived from fibroblast cells transfected by the novel technique of nucleofection or standard lipofection. Theriogenology, 70: 248-259.Search in Google Scholar

Skrzyszowska M., Samiec M., Smor ąg Z. (2005). The effect of in vitro culture conditions on the developmental potential of porcine nuclear transfer embryos reconstructed with cumulus cells. Ann. Anim. Sci., 5: 99-109.Search in Google Scholar

Skrzyszowska M., Samiec M., Smor ąg Z., Lipi ński D., S łomski R. (2007). Creation of porcine transgenic nuclear-transferred embryos reconstructed with e GFP-expressing adult dermal fibroblast cells analyzed on apoptosis. Reprod. Fertil. Dev., 19: 160-161.Search in Google Scholar

Uhm S.J., Chung H.M., Kim C., Shim H., Kim N.-H., Lee H.T., Chung K.S. (2000). In vitro development of porcine enucleated oocytes reconstructed by the transfer of porcine fetal fibroblasts and cumulus cells. Theriogenology, 54: 559-570.1107113010.1016/S0093-691X(00)00371-XSearch in Google Scholar

Wakayama T., Perry A.C.F., Zuccotti M., Johnson K.R., Yanagimachi R. (1998). Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394: 369-374.969047110.1038/286159690471Search in Google Scholar

Yin X.J., Tani T., Yonemura I., Kawakami M., Miyamoto K., Hasegawa R., Kato Y., Tsunoda Y. (2002). Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes. Biol. Reprod., 67: 442-446.1213587910.1095/biolreprod67.2.44212135879Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo