<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction</bold>: Stroke is one of the leading causes of death in Romania. Evidence in supporting the role of the pro-inflammatory cytokine TNFalpha in ischemic pathogenesis is now well established. The aim of the present study is to evaluate the relationship between TNFα -308G>A polymorphism and ischemic stroke in a Northern Romanian population group and to determine whether it has an influence on the risk of cerebral events. This is a cross-sectional, randomized, case-control study for the evaluation of TNFα -308G>A polymorphism alleles frequency among patients with ischemic stroke.<bold>Material and method:</bold> The study included 108 patients diagnosed with ischemic stroke (neurological and CT scan examination), and 118 healthy unrelated controls. TNFα -308G>A genotyping was carried out using PCR-RFLP technique. The amplification of the relevant gene fragment was subjected to restriction enzyme digestion, followed by gel electrophoresis.<bold>Results:</bold> Molecular analysis did not reveal an increased frequency of GA mutant genotype in the study group compared to the control group (p = 0.879, OR = 0.928, CI = 0.512-1.682).<bold>Conclusions:</bold> We found no significant differences in distribution of the TNFα -308G>A polymorphism between ischemic stroke patients and controls. </abstract>

Introduction: Stroke is one of the leading causes of death in Romania. Evidence in supporting the role of the pro-inflammatory cytokine TNFalpha in ischemic pathogenesis is now well established. The aim of the present study is to evaluate the relationship between TNFα -308G>A polymorphism and ischemic stroke in a Northern Romanian population group and to determine whether it has an influence on the risk of cerebral events. This is a cross-sectional, randomized, case-control study for the evaluation of TNFα -308G>A polymorphism alleles frequency among patients with ischemic stroke.Material and method: The study included 108 patients diagnosed with ischemic stroke (neurological and CT scan examination), and 118 healthy unrelated controls. TNFα -308G>A genotyping was carried out using PCR-RFLP technique. The amplification of the relevant gene fragment was subjected to restriction enzyme digestion, followed by gel electrophoresis.Results: Molecular analysis did not reveal an increased frequency of GA mutant genotype in the study group compared to the control group (p = 0.879, OR = 0.928, CI = 0.512-1.682).Conclusions: We found no significant differences in distribution of the TNFα -308G>A polymorphism between ischemic stroke patients and controls.

Genetic Polymorphism TNFα -308G&gt;A and Ischemic Stroke in Northern Romania

Department of Medical Genetics, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

Department of Internal Medicine II, Cluj-Napoca, Romania

Acta Marisiensis - Seria Medica

Genetic Polymorphism TNFα -308G>A and Ischemic Stroke in Northern Romania

{"article-title":"Genetic Polymorphism TNFα -308G>A and Ischemic Stroke in Northern Romania"}

Introduction: Stroke is one of the leading causes of death in Romania. Evidence in supporting the role of the pro-inflammatory cytokine TNFalpha in ischemic...

Genetic Polymorphism TNFα -308G>A and Ischemic Stroke in Northern Romania

Published Online: Aug 13, 2013

Page range: 75 - 77

DOI: https://doi.org/10.2478/amma-2013-0016

This content is open access.