Environmental exposure to glyphosate does not inhibit human acetylcholinesterase and butyrylcholinesterase

1. Duke SO. The history and current status of glyphosate. Pest Manag Sci 2018;74:1027–34. doi: 10.1002/ps.4652 DukeSO The history and current status of glyphosate Pest Manag Sci 2018 74 1027 34 10.1002/ps.4652 Open DOISearch in Google Scholar

2. Steinrücken HC, Amrhein N. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase. Biochem Biophys Res Commun 1980;94:1207–12. doi: 10.1016/0006-291x(80)90547-1 SteinrückenHC AmrheinN The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase Biochem Biophys Res Commun 1980 94 1207 12 10.1016/0006-291x(80)90547-1 Open DOISearch in Google Scholar

3. Defarge N, de Vendômois JS, Séralini GE. Toxicity of formulants and heavy metals in glyphosate-based herbicides and other pesticides. Toxicol Rep 2017;5:156–63. doi: 10.1016/j.toxrep.2017.12.025 DefargeN de VendômoisJS SéraliniGE Toxicity of formulants and heavy metals in glyphosate-based herbicides and other pesticides Toxicol Rep 2017 5 156 63 10.1016/j.toxrep.2017.12.025 Open DOISearch in Google Scholar

4. Silva V, Montanarella L, Jones A, Fernández-Ugalde O, Mol HGJ, Ritsema CJ, Geissen V. Distribution of glyphosate and aminomethylphosphonic acid (AMPA) in agricultural topsoils of the European Union. Sci Total Environ 2018;621:1352–9. doi: 10.1016/j.scitotenv.2017.10.093 SilvaV MontanarellaL JonesA Fernández-UgaldeO MolHGJ RitsemaCJ GeissenV Distribution of glyphosate and aminomethylphosphonic acid (AMPA) in agricultural topsoils of the European Union Sci Total Environ 2018 621 1352 9 10.1016/j.scitotenv.2017.10.093 Open DOISearch in Google Scholar

5. Guyton KZ, Loomis D, Grosse Y, El Ghissassi F, Benbrahim-Tallaa L, Guha N, Scoccianti C, Mattock H, Straif K; International Agency for Research on Cancer Monograph Working Group, IARC, Lyon, France. Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate. Lancet Oncol 2015;16:490–1. doi: 10.1016/S1470-2045(15)70134-8 GuytonKZ LoomisD GrosseY El GhissassiF Benbrahim-TallaaL GuhaN ScocciantiC MattockH StraifK International Agency for Research on Cancer Monograph Working Group, IARC, Lyon, France Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate Lancet Oncol 2015 16 490 1 10.1016/S1470-2045(15)70134-8 Open DOISearch in Google Scholar

6. Lacroix R, Kurrasch DM. Glyphosate toxicity: in vivo, in vitro, and epidemiological evidence. Toxicol Sci 2023;192:131–40. doi: 10.1093/toxsci/kfad018 LacroixR KurraschDM Glyphosate toxicity: in vivo, in vitro, and epidemiological evidence Toxicol Sci 2023 192 131 40 10.1093/toxsci/kfad018 Open DOISearch in Google Scholar

7. Matozzo V, Fabrello J, Marin MG. The effects of glyphosate and its commercial formulations to marine invertebrates: a review. J Mar Sci Eng 2020;8(6):399. doi: 10.3390/jmse8060399 MatozzoV FabrelloJ MarinMG The effects of glyphosate and its commercial formulations to marine invertebrates: a review J Mar Sci Eng 2020 8 6 399 10.3390/jmse8060399 Open DOISearch in Google Scholar

8. Gillezeau C, van Gerwen M, Shaffer RM, Rana I, Zhang L, Sheppard L, Taioli E. The evidence of human exposure to glyphosate: a review. Environ Health 2019;18(1):2. doi: 10.1186/s12940-018-0435-5 GillezeauC van GerwenM ShafferRM RanaI ZhangL SheppardL TaioliE The evidence of human exposure to glyphosate: a review Environ Health 2019 18 1 2 10.1186/s12940-018-0435-5 Open DOISearch in Google Scholar

9. Timperley CM, Forman JE, Abdollahi M, Al-Amri AS, Baulig A, Benachour D, Borrett V, Cariño FA, Geist M, Gonzalez D, Kane W, Kovarik Z, Martínez-Álvarez R, Mourão NMF, Neffe S, Raza SK, Rubaylo V, Suárez AG, Takeuchi K, Tang C, Trifirò F, van Straten FM, Vanninen PS, Vučinić S, Zaitsev V, Zafar-Uz-Zaman M, Zina MS, Holen S. Advice on assistance and protection provided by the Scientific Advisory Board of the Organisation for the Prohibition of Chemical Weapons: Part 1. On medical care and treatment of injuries from nerve agents. Toxicology 2019;415:56–69. doi: 10.1016/j.tox.2019.01.004 TimperleyCM FormanJE AbdollahiM Al-AmriAS BauligA BenachourD BorrettV CariñoFA GeistM GonzalezD KaneW KovarikZ Martínez-ÁlvarezR MourãoNMF NeffeS RazaSK RubayloV SuárezAG TakeuchiK TangC TrifiròF van StratenFM VanninenPS VučinićS ZaitsevV Zafar-Uz-ZamanM ZinaMS HolenS Advice on assistance and protection provided by the Scientific Advisory Board of the Organisation for the Prohibition of Chemical Weapons: Part 1. On medical care and treatment of injuries from nerve agents Toxicology 2019 415 56 69 10.1016/j.tox.2019.01.004 Open DOISearch in Google Scholar

10. Naughton SX, Terry AV Jr. Neurotoxicity in acute and repeated organophosphate exposure. Toxicology 2018;408:101–12. doi: 10.1016/j.tox.2018.08.011 NaughtonSX TerryAVJr Neurotoxicity in acute and repeated organophosphate exposure Toxicology 2018 408 101 12 10.1016/j.tox.2018.08.011 Open DOISearch in Google Scholar

11. Kwiatkowska M, Nowacka-Krukowska H, Bukowska B. The effect of glyphosate, its metabolites and impurities on erythrocyte acetylcholinesterase activity. Environ Toxicol Pharmacol 2014;37:1101–8. doi: 10.1016/j.etap.2014.04.008 KwiatkowskaM Nowacka-KrukowskaH BukowskaB The effect of glyphosate, its metabolites and impurities on erythrocyte acetylcholinesterase activity Environ Toxicol Pharmacol 2014 37 1101 8 10.1016/j.etap.2014.04.008 Open DOISearch in Google Scholar

12. Milić M, Žunec S, Micek V, Kašuba V, Mikolić A, Lovaković BT, Živković Semren T, Pavičić I, Čermak AMM, Pizent A, Lucić Vrdoljak A, Valencia-Quintana R, Sánchez-Alarcón J, Želježić D. Oxidative stress, cholinesterase activity, and DNA damage in the liver, whole blood, and plasma of Wistar rats following a 28-day exposure to glyphosate. Arh Hig Rada Toksikol 2018;69:154–68. doi: 10.2478/aiht-2018-69-3114 MilićM ŽunecS MicekV KašubaV MikolićA LovakovićBT Živković SemrenT PavičićI ČermakAMM PizentA Lucić VrdoljakA Valencia-QuintanaR Sánchez-AlarcónJ ŽelježićD Oxidative stress, cholinesterase activity, and DNA damage in the liver, whole blood, and plasma of Wistar rats following a 28-day exposure to glyphosate Arh Hig Rada Toksikol 2018 69 154 68 10.2478/aiht-2018-69-3114 Open DOISearch in Google Scholar

13. Martins-Gomes C, Coutinho T E, Silva TL, Andreani T, Silva AM. Neurotoxicity assessment of four different pesticides using in vitro enzymatic inhibition assays. Toxics 2022;10(8):448. doi: 10.3390/toxics10080448 Martins-GomesC CoutinhoTE SilvaTL AndreaniT SilvaAM Neurotoxicity assessment of four different pesticides using in vitro enzymatic inhibition assays Toxics 2022 10 8 448 10.3390/toxics10080448 Open DOISearch in Google Scholar

14. Čadež T, Kolić D, Šinko G, Kovarik Z. Assessment of four organophosphorus pesticides as inhibitors of human acetylcholinesterase and butyrylcholinesterase. Sci Rep 2021;11(1):21486. doi: 10.1038/s41598-021-00953-9 ČadežT KolićD ŠinkoG KovarikZ Assessment of four organophosphorus pesticides as inhibitors of human acetylcholinesterase and butyrylcholinesterase Sci Rep 2021 11 1 21486 10.1038/s41598-021-00953-9 Open DOISearch in Google Scholar

15. Pehar V, Kolić D, Zandona A, Šinko G, Katalinić M, Stepanić V, Kovarik Z. Selected herbicides screened for toxicity and analysed as inhibitors of both cholinesterases. Chem Biol Interact 2023;379:110506. doi: 10.1016/j.cbi.2023.110506 PeharV KolićD ZandonaA ŠinkoG KatalinićM StepanićV KovarikZ Selected herbicides screened for toxicity and analysed as inhibitors of both cholinesterases Chem Biol Interact 2023 379 110506 10.1016/j.cbi.2023.110506 Open DOISearch in Google Scholar

16. Kovarik Z, Čalić M, Šinko G, Bosak A. Structure-activity approach in the reactivation of tabun-phosphorylated human acetylcholinesterase with bispyridinium para-aldoximes. Arh Hig Rada Toksikol 2007;58:201–9. doi: 10.2478/v10004-007-0013-7. KovarikZ ČalićM ŠinkoG BosakA Structure-activity approach in the reactivation of tabun-phosphorylated human acetylcholinesterase with bispyridinium para-aldoximes Arh Hig Rada Toksikol 2007 58 201 9 10.2478/v10004-007-0013-7. Open DOISearch in Google Scholar

17. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961;7:88–95. doi: 10.1016/0006-2952(61)90145-9 EllmanGL CourtneyKD AndresV FeatherstoneRM A new and rapid colorimetric determination of acetylcholinesterase activity Biochem Pharmacol 1961 7 88 95 10.1016/0006-2952(61)90145-9 Open DOISearch in Google Scholar

18. Larsen KE, Lifschitz AL, Lanusse CE, Virkel GL. The herbicide glyphosate is a weak inhibitor of acetylcholinesterase in rats. Environ Toxicol Pharmacol 2016;45:41–4. doi: 10.1016/j.etap.2016.05.012 LarsenKE LifschitzAL LanusseCE VirkelGL The herbicide glyphosate is a weak inhibitor of acetylcholinesterase in rats Environ Toxicol Pharmacol 2016 45 41 4 10.1016/j.etap.2016.05.012 Open DOISearch in Google Scholar

19. El-Demerdash FM, Yousef MI, Elagamy EI. Influence of paraquat, glyphosate, and cadmium on the activity of some serum enzymes and protein electrophoretic behavior (in vitro). J Environ Sci Health Part B 2001;36:29–42. doi: 10.1081/pfc-100000914 El-DemerdashFM YousefMI ElagamyEI Influence of paraquat, glyphosate, and cadmium on the activity of some serum enzymes and protein electrophoretic behavior (in vitro) J Environ Sci Health Part B 2001 36 29 42 10.1081/pfc-100000914 Open DOISearch in Google Scholar

20. Takeuchi I, Yanagawa Y, Nagasawa H, Jitsuiki K, Madokoro S, Takahashi N, Ohsaka H, Ishikawa K, Omori K. Decrease in butyrylcholinesterase accompanied by intermediate-like syndrome after massive ingestion of a glyphosate-surfactant. Intern Med 2019;58:3057–59. doi: 10.2169/internalmedicine.2562-18 TakeuchiI YanagawaY NagasawaH JitsuikiK MadokoroS TakahashiN OhsakaH IshikawaK OmoriK Decrease in butyrylcholinesterase accompanied by intermediate-like syndrome after massive ingestion of a glyphosate-surfactant Intern Med 2019 58 3057 59 10.2169/internalmedicine.2562-18 Open DOISearch in Google Scholar

21. Richardson JR, Fitsanakis V, Westerink RHS, Kanthasamy AG. Neurotoxicity of pesticides. Acta Neuropathol 2019;138:343–62. doi: 10.1007/s00401-019-02033-9 RichardsonJR FitsanakisV WesterinkRHS KanthasamyAG Neurotoxicity of pesticides Acta Neuropathol 2019 138 343 62 10.1007/s00401-019-02033-9 Open DOISearch in Google Scholar

22. Zandona A, Zorbaz T, Miš K, Pirkmajer S, Katalinić M. Cytotoxicity-related effects of imidazolium and chlorinated bispyridinium oximes in SH-SY5Y cells. Arh Hig Rada Toksikol 2022;73:277–84. doi: 10.2478/aiht-2022-73-3688 ZandonaA ZorbazT MišK PirkmajerS KatalinićM Cytotoxicity-related effects of imidazolium and chlorinated bispyridinium oximes in SH-SY5Y cells Arh Hig Rada Toksikol 2022 73 277 84 10.2478/aiht-2022-73-3688 Open DOISearch in Google Scholar

23. Costas-Ferriera C, Durán R, Faro LRF. Toxic Effects of glyphosate on the nervous system: A systematic review. Int J Mol Sci 2022;23:4605. doi: 10.3390/ijms23094605 Costas-FerrieraC DuránR FaroLRF Toxic Effects of glyphosate on the nervous system: A systematic review Int J Mol Sci 2022 23 4605 10.3390/ijms23094605 Open DOISearch in Google Scholar

24. Martínez MA, Rodríguez JL, Lopez-Torres B, Martínez M, Martínez-Larrañaga MR, Maximiliano JE, Anadón A, Ares I. Use of human neuroblastoma SH-SY5Y cells to evaluate glyphosate-induced effects on oxidative stress, neuronal development and cell death signaling pathways. Environ Int 2020;135:105414. doi: 10.1016/j.envint.2019.105414 MartínezMA RodríguezJL Lopez-TorresB MartínezM Martínez-LarrañagaMR MaximilianoJE AnadónA AresI Use of human neuroblastoma SH-SY5Y cells to evaluate glyphosate-induced effects on oxidative stress, neuronal development and cell death signaling pathways Environ Int 2020 135 105414 10.1016/j.envint.2019.105414 Open DOISearch in Google Scholar

25. Martinez A, Al-Ahmad AJ. Effects of glyphosate and aminomethylphosphonic acid on an isogeneic model of the human blood-brain barrier. Toxicol Lett 2019;304:39–49. doi: 10.1016/j.toxlet.2018.12.013 MartinezA Al-AhmadAJ Effects of glyphosate and aminomethylphosphonic acid on an isogeneic model of the human blood-brain barrier Toxicol Lett 2019 304 39 49 10.1016/j.toxlet.2018.12.013 Open DOISearch in Google Scholar

26. Cattani D, de Liz Oliveira Cavalli VL, Rieg CEH, Domingues JT, Dal-Cim T, Tasca CI, Mena Barreto Silva FR, Zamoner A. Mechanisms underlying the neurotoxicity induced by glyphosate-based herbicide in immature rat hippocampus: involvement of glutamate excitotoxicity. Toxicology 2014;320:34–45. doi: 10.1016/j.tox.2014.03.001 CattaniD de Liz Oliveira CavalliVL RiegCEH DominguesJT Dal-CimT TascaCI Mena Barreto SilvaFR ZamonerA Mechanisms underlying the neurotoxicity induced by glyphosate-based herbicide in immature rat hippocampus: involvement of glutamate excitotoxicity Toxicology 2014 320 34 45 10.1016/j.tox.2014.03.001 Open DOISearch in Google Scholar

27. Kovarik Z, Moshitzky G, Maček Hrvat N, Soreq H. Recent advances in cholinergic mechanisms as reactions to toxicity, stress, and neuroimmune insults. J Neurochem 2023. doi: 10.1111/jnc.15887. Online ahead of print KovarikZ MoshitzkyG Maček HrvatN SoreqH Recent advances in cholinergic mechanisms as reactions to toxicity, stress, and neuroimmune insults J Neurochem 2023 10.1111/jnc.15887 Online ahead of print Open DOISearch in Google Scholar

28. Kolić D, Kovarik Z. N-methyl-d-aspartate receptors: Structure, function, and role in organophosphorus compound poisoning. Biofactors 2024. doi: 10.1002/biof.2048. Online ahead of print KolićD KovarikZ N-methyl-d-aspartate receptors: Structure, function, and role in organophosphorus compound poisoning Biofactors 2024 10.1002/biof.2048 Online ahead of print Open DOISearch in Google Scholar

29. Gallegos CE, Bartos M, Gumilar F, Raisman-Vozari R, Minetti A, Baier CJ. Intranasal glyphosate-based herbicide administration alters the redox balance and the cholinergic system in the mouse brain. Neurotoxicology 2020;77:205–15. doi: 10.1016/j.neuro.2020.01.007 GallegosCE BartosM GumilarF Raisman-VozariR MinettiA BaierCJ Intranasal glyphosate-based herbicide administration alters the redox balance and the cholinergic system in the mouse brain Neurotoxicology 2020 77 205 15 10.1016/j.neuro.2020.01.007 Open DOISearch in Google Scholar

30. Baier CJ, Gallegos CE, Raisman-Vozari R, Minetti A. Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice. Neurotoxicol Teratol 2017;64:63–72. doi: 10.1016/j.ntt.2017.10.004 BaierCJ GallegosCE Raisman-VozariR MinettiA Behavioral impairments following repeated intranasal glyphosate-based herbicide administration in mice Neurotoxicol Teratol 2017 64 63 72 10.1016/j.ntt.2017.10.004 Open DOISearch in Google Scholar

31. Bali YA, Kaikai N, Ba-M’hamed S, Bennis M. Learning and memory impairments associated to acetylcholinesterase inhibition and oxidative stress following glyphosate based-herbicide exposure in mice. Toxicology 2019;415:18–25. doi: 10.1016/j.tox.2019.01.010 BaliYA KaikaiN Ba-M’hamedS BennisM Learning and memory impairments associated to acetylcholinesterase inhibition and oxidative stress following glyphosate based-herbicide exposure in mice Toxicology 2019 415 18 25 10.1016/j.tox.2019.01.010 Open DOISearch in Google Scholar

32. Winstone JK, Pathak KV, Winslow W, Piras IS, White J, Sharma R, Huentelman MJ, Pirrote P, Velazquez R. Glyphosate infiltrates the brain and increases pro-inflammatory cytokine TNFα: implications for neurodegenerative disorders. J Neuroinflammation 2022;19(1):193. doi: 10.1186/s12974-022-02544-5 WinstoneJK PathakKV WinslowW PirasIS WhiteJ SharmaR HuentelmanMJ PirroteP VelazquezR Glyphosate infiltrates the brain and increases pro-inflammatory cytokine TNFα: implications for neurodegenerative disorders J Neuroinflammation 2022 19 1 193 10.1186/s12974-022-02544-5 Open DOISearch in Google Scholar