<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_ahem-2021-0032_s_011"><h3>Introduction</h3>
<p>Glutaminolysis, beside glycolysis, is a key metabolic pathway of a cancer cell that provides energy and substrates for the synthesis of nucleic acids, proteins, and lipids. The pathway is mediated by both mitochondrial and cytosolic enzymes. Neither expression of glutaminolysis enzymes in colon cancer cells nor the influence of various oxygen concentrations on their expression has been studied so far.</p></sec>
<sec id="j_ahem-2021-0032_s_012"><h3>Objectives</h3>
<p>The aim of the study was to determine and compare the mRNA expression of enzymes involved in glutaminolysis at various oxygen levels in human primary (SW480) and metastatic (SW620) colon cancer cells cultured in 1% O<sub>2</sub> (hypoxia), 10% O<sub>2</sub> (tissue normoxia), 21% O<sub>2</sub> (atmospheric normoxia).</p></sec>
<sec id="j_ahem-2021-0032_s_013"><h3>Methods</h3>
<p>Cell viability was determined by Trypan Blue exclusion (TB) and Thiazolyl Blue Tetrazolium Bromide (MTT). The expression of <italic>HIF1α, GLUT1, GLS1, AST1, AST2, ACL, PC</italic> and <italic>GC1, GC2</italic> at mRNA levelwas determined by RT-qPCR. Results. Correlation between increasing oxygen concentration and cell count was not observed. In both cell lines the number of viable cells was the lowest at 10% oxygen. The enzyme profile and expression of proteins involved in glutaminolysis varied depending on oxygen pressure and type of cell lines. In summary, our findings suggest differences in metabolic adaptation to oxygen availability <italic>in vivo</italic> between primary and metastatic colon cancer cells.</p></sec></abstract>

Introduction
Glutaminolysis, beside glycolysis, is a key metabolic pathway of a cancer cell that provides energy and substrates for the synthesis of nucleic acids, proteins, and lipids. The pathway is mediated by both mitochondrial and cytosolic enzymes. Neither expression of glutaminolysis enzymes in colon cancer cells nor the influence of various oxygen concentrations on their expression has been studied so far.
Objectives
The aim of the study was to determine and compare the mRNA expression of enzymes involved in glutaminolysis at various oxygen levels in human primary (SW480) and metastatic (SW620) colon cancer cells cultured in 1% O2 (hypoxia), 10% O2 (tissue normoxia), 21% O2 (atmospheric normoxia).
Methods
Cell viability was determined by Trypan Blue exclusion (TB) and Thiazolyl Blue Tetrazolium Bromide (MTT). The expression of HIF1α, GLUT1, GLS1, AST1, AST2, ACL, PC and GC1, GC2 at mRNA levelwas determined by RT-qPCR. Results. Correlation between increasing oxygen concentration and cell count was not observed. In both cell lines the number of viable cells was the lowest at 10% oxygen. The enzyme profile and expression of proteins involved in glutaminolysis varied depending on oxygen pressure and type of cell lines. In summary, our findings suggest differences in metabolic adaptation to oxygen availability in vivo between primary and metastatic colon cancer cells.

Influence of oxygen availability on expression of glutaminolysis genes in human colon cancer cells

Postępy Higieny i Medycyny Doświadczalnej

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Introduction
Glutaminolysis, beside glycolysis, is a key metabolic pathway of a cancer cell that provides energy and substrates for the synthesis of nucleic...

Influence of oxygen availability on expression of glutaminolysis genes in human colon cancer cells

Article Category: Review

Published Online: Dec 20, 2021

Page range: 923 - 932

Received: Nov 09, 2020

Accepted: Jul 29, 2021

DOI: https://doi.org/10.2478/ahem-2021-0032

Keywords
Colorectal cancer, glutaminolysis, hypoxia, tissue normoxia

© 2021 Otto-Ślusarczyk., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of oxygen availability on expression of glutaminolysis genes in human colon cancer cells

Article Category: Review

Published Online: Dec 20, 2021

Page range: 923 - 932

Received: Nov 09, 2020

Accepted: Jul 29, 2021

DOI: https://doi.org/10.2478/ahem-2021-0032

KeywordsColorectal cancer, glutaminolysis, hypoxia, tissue normoxia

© 2021 Otto-Ślusarczyk., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Colorectal cancer, glutaminolysis, hypoxia, tissue normoxia