<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Taurine (2-aminoethanesulfonic acid) is a free sulfur-containing β-amino acid widely distributed in many mammalians. Owing to the energizing effects, it is mostly used in soft drinks and supplements for athletes. Regular intake of soft drinks may lead to an overdose of caffeine, taurine, and guarana and loss of bone mass, overweight, hypertension, and in older age, osteoporosis and cardiovascular diseases. Therefore, it is essential to control the maximum amount of taurine consumed by humans in the food and beverages. Here, a fast, simple, accurate, and robust method based on ultrahigh-performance liquid chromatography hyphenated with mass spectrometry (UHPLC-MS) was successfully applied for the determination of taurine in selected soft drinks sold in Slovakia. The method was characterized by coefficient of determination higher than 0.99, and the predicted value of the limit of detection was 4.29 μmol/L. The analyzed levels of taurine in selected commercial drinks ranged from 2.8 to 3.78 mg/mL. The concentration in one brand of the investigated drinks was found to be extremely low (about 70%) compared to the declared content by the manufacturer.</p>
</abstract>

Taurine (2-aminoethanesulfonic acid) is a free sulfur-containing β-amino acid widely distributed in many mammalians. Owing to the energizing effects, it is mostly used in soft drinks and supplements for athletes. Regular intake of soft drinks may lead to an overdose of caffeine, taurine, and guarana and loss of bone mass, overweight, hypertension, and in older age, osteoporosis and cardiovascular diseases. Therefore, it is essential to control the maximum amount of taurine consumed by humans in the food and beverages. Here, a fast, simple, accurate, and robust method based on ultrahigh-performance liquid chromatography hyphenated with mass spectrometry (UHPLC-MS) was successfully applied for the determination of taurine in selected soft drinks sold in Slovakia. The method was characterized by coefficient of determination higher than 0.99, and the predicted value of the limit of detection was 4.29 μmol/L. The analyzed levels of taurine in selected commercial drinks ranged from 2.8 to 3.78 mg/mL. The concentration in one brand of the investigated drinks was found to be extremely low (about 70%) compared to the declared content by the manufacturer.

Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method

Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy

Biomedical Research Center Slovak Academy of Sciences

Toxicological and Antidoping Center, Faculty of Pharmacy

Department of Galenic Pharmacy, Faculty of Pharmacy

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Taurine (2-aminoethanesulfonic acid) is a free sulfur-containing β-amino acid widely distributed in many mammalians. Owing to the energizing effects, it is...

Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method

Article Category: Special Issue Article

Published Online: Aug 31, 2023

Page range: 67 - 74

Received: May 30, 2023

Accepted: Jun 23, 2023

DOI: https://doi.org/10.2478/afpuc-2023-0010

Keywords
taurine, soft drinks, liquid chromatography, food and food supplements quality control, mass spectrometry

© 2023 P. Chalova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method

Article Category: Special Issue Article

Published Online: Aug 31, 2023

Page range: 67 - 74

Received: May 30, 2023

Accepted: Jun 23, 2023

DOI: https://doi.org/10.2478/afpuc-2023-0010

Keywordstaurine, soft drinks, liquid chromatography, food and food supplements quality control, mass spectrometry

© 2023 P. Chalova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
taurine, soft drinks, liquid chromatography, food and food supplements quality control, mass spectrometry