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N-glycans Profiling in Pilocarpine Induced Status Epilepticus in Immature Rats

S. Kapoor

S. Kapoor

  • Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of SciencesBratislava, Slovak Republic
  • Institute of Physiology, Czech Academy of SciencesPrague, Czech Republic
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Kapoor, S.
, 
M. Nemcovic

M. Nemcovic

Institute of Chemistry, Slovak Academy of SciencesBratislava, Slovak Republic
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Nemcovic, M.
, 
J. Folbergrova

J. Folbergrova

Institute of Physiology, Czech Academy of SciencesPrague, Czech Republic
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Folbergrova, J.
, 
D. Kala

D. Kala

Institute of Physiology, Czech Academy of SciencesPrague, Czech Republic
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Kala, D.
, 
J. Svoboda

J. Svoboda

Institute of Physiology, Czech Academy of SciencesPrague, Czech Republic
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Svoboda, J.
, 
J. Otahal

J. Otahal

Institute of Physiology, Czech Academy of SciencesPrague, Czech Republic
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Otahal, J.
 and 
Z. Brnoliakova

Z. Brnoliakova

Centre of Experimental Medicine, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of SciencesBratislava, Slovak Republic
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Brnoliakova, Z.
  
Aug 11, 2022
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Article Category: Original Paper
Published Online: Aug 11, 2022
Page range: 1 - 4
Received: Feb 09, 2022
Accepted: May 26, 2022
DOI: https://doi.org/10.2478/afpuc-2022-0011
Keywords
© 2022 S. Kapoor et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

The relative intensities of N-glycans in the experimental model of lithium pilocarpine (Li-Pilo) induced status epilepticus (SE) in blood sera in immature rats. N-glycans were assorted according to (a) sialylation and non-sialylation; (b) their glycan type. Explanatory notes: High-Man – high-mannose type of N-glycan; C-Bi – complex bi-antennary; C-Bi-Fuc – complex bi-antennary with Fucose; C-Tri – complex tri-antennary; C-Tri-Fuc – complex tri-antennary with Fucose; C-Tetra – complex tetra-antennary; C-Tetra-Fuc – complex tetra-antennary with Fucose; Hybrid – hybrid type of N-glycan. Data were evaluated using GraphPad Software. Unpaired t-test with two tailed analysis was used to evaluate the difference among the experimental groups. The statistically significant difference are marked as * for p < 0.05 and ** for p < 0.01. Data were expressed as means ± SEM.
The relative intensities of N-glycans in the experimental model of lithium pilocarpine (Li-Pilo) induced status epilepticus (SE) in blood sera in immature rats. N-glycans were assorted according to (a) sialylation and non-sialylation; (b) their glycan type. Explanatory notes: High-Man – high-mannose type of N-glycan; C-Bi – complex bi-antennary; C-Bi-Fuc – complex bi-antennary with Fucose; C-Tri – complex tri-antennary; C-Tri-Fuc – complex tri-antennary with Fucose; C-Tetra – complex tetra-antennary; C-Tetra-Fuc – complex tetra-antennary with Fucose; Hybrid – hybrid type of N-glycan. Data were evaluated using GraphPad Software. Unpaired t-test with two tailed analysis was used to evaluate the difference among the experimental groups. The statistically significant difference are marked as * for p < 0.05 and ** for p < 0.01. Data were expressed as means ± SEM.

The list of detected N-glycans sorted according to their structural type_

Glycan type m/z
High-Man 1579.8; 1783.5; 1988.1; 2192.2; 2396.4
C-Bi 2227.4; 2431.5; 2736.8; 2792.7
C-Bi-Fuc 1836.1; 2040.2; 2605.7; 2966.9
C-Tri 3154.1; 3603.2
C-Tri-Fuc 2081.2; 3328.1; 3777.4
C-Tetra 3964.3
C-Tetra-Fuc 4138.4
Hybrid 2390.4
Language:
English
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Pharmacy, Pharmacy, other
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