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Study of the solubilisation process of bacterial model membranes induced by DDAO

K. Želinská
K. Želinská
 and 
J. Gallová
J. Gallová
   | May 11, 2021
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Article Category: Original Paper
Published Online: May 11, 2021
Page range: 17 - 23
Received: Nov 30, 2020
Accepted: Mar 01, 2021
DOI: https://doi.org/10.2478/afpuc-2020-0019
Keywords
© 2021 Želinská K. et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Dependence of normalised intensity of scattered light (INORM) on the concentration of added DDAO (cDDAO), both types of membrane at a lipid concentration of 0.1 mmol/dm3. Inset shows a comparison of the experiment at three different concentrations of POPE-POPG-TOCL (intensity (I) was not normalised).
Dependence of normalised intensity of scattered light (INORM) on the concentration of added DDAO (cDDAO), both types of membrane at a lipid concentration of 0.1 mmol/dm3. Inset shows a comparison of the experiment at three different concentrations of POPE-POPG-TOCL (intensity (I) was not normalised).

Figure 2

Dependence of scattered light intensity (I) on the concentration of added DDAO, cPOPE+POPG+TOCL = 0.06 mmol/dm3. Arrows indicate the critical concentrations DtSAT, DtSOL acquired using bilinear functions (full line) and DtMID using reverse sigmoid function (dashed line).
Dependence of scattered light intensity (I) on the concentration of added DDAO, cPOPE+POPG+TOCL = 0.06 mmol/dm3. Arrows indicate the critical concentrations DtSAT, DtSOL acquired using bilinear functions (full line) and DtMID using reverse sigmoid function (dashed line).

Figure 3

Linear dependencies of critical DDAO concentrations on the concentration of POPE-POPG (left) and POPE-POPG-TOCL (right). The figure shows the global fit used to calculate the effective molar ratios (Re ) and the partition coefficients (Kp). DwSAT represents the concentration of DDAO surfactant in the water phase at the stage of complete saturation of the lipid bilayers by DDAO.
Linear dependencies of critical DDAO concentrations on the concentration of POPE-POPG (left) and POPE-POPG-TOCL (right). The figure shows the global fit used to calculate the effective molar ratios (Re ) and the partition coefficients (Kp). DwSAT represents the concentration of DDAO surfactant in the water phase at the stage of complete saturation of the lipid bilayers by DDAO.

Global fit (Equation 3) results of POPE-POPG and POPE-POPG-TOCL static light scattering data.

Critical DDAO concentration POPE-POPG POPE-POPG-TOCL
Re DW [mmol/dm3] Re DW [mmol/dm3]
D tSAT 3.7 ± 0.2 0.9 ± 0.1 5.0 ± 0.3 1.0 ± 0.1
DtMID 4.6 ± 0.3 1.2 ± 0.2 6.3 ± 0.3 1.2 ± 0.2
DtSOL 5.1 ± 0.3 1.3 ± 0.2 7.5 ± 0.4 1.4 ± 0.2
Kp 5,300 ± 400 6,500 ± 500
eISSN:
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Language:
English
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Journal Subjects:
Pharmacy, other
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