<abstract xmlns="http://www.w3.org/1999/xhtml">

Solubilisation of two bacterial model membranes induced by <italic>N,N-</italic>dimethyl-1-dodecanamine-<italic>N-</italic>oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a second model membrane was enriched with tetraoleoylcardiolipin (TOCL) with a composition POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol. Solubilisation of these model membranes was studied by static light scattering (nephelometry). Effective ratio Re (the amount of DDAO integrated into the bilayer to the amount of lipid) at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO was calculated – in case of the POPE-POPG membrane, Kp = 5,300 ± 400, for the POPE-POPG-TOCL membrane, Kp = 6,500 ± 500.
</abstract>

Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a second model membrane was enriched with tetraoleoylcardiolipin (TOCL) with a composition POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol. Solubilisation of these model membranes was studied by static light scattering (nephelometry). Effective ratio Re (the amount of DDAO integrated into the bilayer to the amount of lipid) at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO was calculated – in case of the POPE-POPG membrane, Kp = 5,300 ± 400, for the POPE-POPG-TOCL membrane, Kp = 6,500 ± 500.

Study of the solubilisation process of bacterial model membranes induced by DDAO

Department of Physical Chemistry of Drugs, Faculty of Pharmacy

European Pharmaceutical Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Study of the solubilisation process of bacterial model membranes induced by DDAO"}

Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a...

Study of the solubilisation process of bacterial model membranes induced by DDAO

Article Category: Original Paper

Published Online: May 11, 2021

Page range: 17 - 23

Received: Nov 30, 2020

Accepted: Mar 01, 2021

DOI: https://doi.org/10.2478/afpuc-2020-0019

Keywords
N,N-dimethyl-1-dodecanamine-N-oxide, solubilisation, bacterial model membranes, cardiolipin

© 2021 Želinská K. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Study of the solubilisation process of bacterial model membranes induced by DDAO

Article Category: Original Paper

Published Online: May 11, 2021

Page range: 17 - 23

Received: Nov 30, 2020

Accepted: Mar 01, 2021

DOI: https://doi.org/10.2478/afpuc-2020-0019

KeywordsN,N-dimethyl-1-dodecanamine-N-oxide, solubilisation, bacterial model membranes, cardiolipin

© 2021 Želinská K. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
N,N-dimethyl-1-dodecanamine-N-oxide, solubilisation, bacterial model membranes, cardiolipin