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Concentration-dependent effect of silymarin on concanavalin A-stimulated mouse spleen cells in vitro

G. Hrčková

G. Hrčková

Institute of Parasitology of the Slovak, Academy of SciencesKošice, Slovakia
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Hrčková, G.
, 
T. Mačák Kubašková

T. Mačák Kubašková

Institute of Parasitology of the Slovak, Academy of SciencesKošice, Slovakia
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Mačák Kubašková, T.
, 
D. Mudroňová

D. Mudroňová

Institute of Microbiology and Immunology, The University of Veterinary Medicine and PharmacyKošice, Slovakia
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Mudroňová, D.
 and 
A. Bardelčíková

A. Bardelčíková

Annamária Bardelčíková, Department of Pharmacology, Faculty of Medicine, The University of P. J. ŠafárikKošice, Slovakia
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Bardelčíková, A.
  
Nov 18, 2020
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Article Category: Original research article/Review
Published Online: Nov 18, 2020
Page range: 17 - 26
Received: Oct 02, 2019
Accepted: Dec 10, 2019
DOI: https://doi.org/10.2478/afpuc-2020-0003
Keywords
© 2019 Hrčková G et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

The effect of silymarin on CoA-activated mouse spleen cells in vitro: (a) proliferation index of T lymphocytes; (b) viability test of T lymphocytes using trypan blue staining. Significantly different values between CoA alone and CoA + silymarin treated groups are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.
The effect of silymarin on CoA-activated mouse spleen cells in vitro: (a) proliferation index of T lymphocytes; (b) viability test of T lymphocytes using trypan blue staining. Significantly different values between CoA alone and CoA + silymarin treated groups are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.

Figure 2

The effect of silymarin on cytokine secretion and mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro: (a) concentration of IFN-γ and (b) IL-4 in supernatants of cells after 70 h cultivation; (c) relative gene expression for IFN-γ and (d) IL-4 in T lymphocytes after 70 h of cultivation. Significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.
The effect of silymarin on cytokine secretion and mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro: (a) concentration of IFN-γ and (b) IL-4 in supernatants of cells after 70 h cultivation; (c) relative gene expression for IFN-γ and (d) IL-4 in T lymphocytes after 70 h of cultivation. Significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.

Figure 3

The effect of silymarin on mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro for (a) transcription factor NF-κB and (b) transcription factor Foxp3. Significantly different values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.
The effect of silymarin on mRNA levels in control unstimulated splenocytes and CoA-activated mouse spleen T lymphocytes in vitro for (a) transcription factor NF-κB and (b) transcription factor Foxp3. Significantly different values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001.

Figure 4

The effect of silymarin on mitochondrial membrane potential in naïve and CoA-activated mouse spleen cells and after treatment with silymarin (b), expressed as the mean intensity of fluorescence (MIF) for Rhodamine 123: (a) representative plots of MIF for control and individual silymarin-treated groups. Significantly different values are indicated as: *p < 0.05.
The effect of silymarin on mitochondrial membrane potential in naïve and CoA-activated mouse spleen cells and after treatment with silymarin (b), expressed as the mean intensity of fluorescence (MIF) for Rhodamine 123: (a) representative plots of MIF for control and individual silymarin-treated groups. Significantly different values are indicated as: *p < 0.05.

Figure 5

The proportions of live, early apoptotic, and late apoptotic/dead cells determined for the suspensions of naïve splenocytes, CoA-activated T lymphocytes, and following in vitro treatment with silymarin after 70 h of cultivation (b). Representative flow cytometric dot plots showing Annexin V and propidium iodide staining of naïve splenocytes and CoA-activated cells following silymarin treatment (a). Significantly different values between CoA alone and silymarin-treated groups for early apoptotic cells are indicated as *p < 0.05 and for live cells are indicated as Ñp < 0.05.
The proportions of live, early apoptotic, and late apoptotic/dead cells determined for the suspensions of naïve splenocytes, CoA-activated T lymphocytes, and following in vitro treatment with silymarin after 70 h of cultivation (b). Representative flow cytometric dot plots showing Annexin V and propidium iodide staining of naïve splenocytes and CoA-activated cells following silymarin treatment (a). Significantly different values between CoA alone and silymarin-treated groups for early apoptotic cells are indicated as *p < 0.05 and for live cells are indicated as Ñp < 0.05.
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