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Study of qualitative and quantitative content of amino acids in pumpkin seeds for further standardization of the herbal drug

E.E. Kotova

E.E. Kotova

Department of the State Pharmacopoeia of Ukraine, State enterprise “Ukrainian scientific Pharmacopoeial center for quality of medicines”Kharkiv, Ukraine
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Kotova, E.E.
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S.A. Kotov

S.A. Kotov

Department of the State Pharmacopoeia of Ukraine, State enterprise “Ukrainian scientific Pharmacopoeial center for quality of medicines”Kharkiv, Ukraine
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Kotov, S.A.
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T.M. Gontova

T.M. Gontova

Department of Botany, National University of Pharmacy4, Valentynivska Str., Ukraine
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Gontova, T.M.
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A.G. Kotov

A.G. Kotov

Department of the State Pharmacopoeia of Ukraine, State enterprise “Ukrainian scientific Pharmacopoeial center for quality of medicines”Kharkiv, Ukraine
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Nov 18, 2020
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Article Category: Original Paper
Published Online: Nov 18, 2020
Page range: 27 - 32
Received: Sep 05, 2019
Accepted: Nov 27, 2019
DOI: https://doi.org/10.2478/afpuc-2020-0001
Keywords
© 2019 Kotova E.E. et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Pumpkin seeds: dried drug substance
Pumpkin seeds: dried drug substance

Figure 2

The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 1,2,3,4, test solutions obtained from different series (RS 799, 800, 801, 802); RS 1, solution of arginine (zone with lower Rf) and glycine (zone with higher Rf); RS 2, solution of alanine (zone with lower Rf) and leucine (zone with higher Rf); RS 3, solution of aspartic acid (zone with lower Rf) and solution of glutamic acid (zone with higher Rf)
The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 1,2,3,4, test solutions obtained from different series (RS 799, 800, 801, 802); RS 1, solution of arginine (zone with lower Rf) and glycine (zone with higher Rf); RS 2, solution of alanine (zone with lower Rf) and leucine (zone with higher Rf); RS 3, solution of aspartic acid (zone with lower Rf) and solution of glutamic acid (zone with higher Rf)

Figure 3

The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 5,6,7, test solutions obtained from different series (RS 803, 804, 805); RS 4, solution of histidine, aspartic acid, glutamic acid, valine (in order of increasing Rf); RS 5, solution of arginine, glycine, alanine, and leucine (in order of increasing Rf)
The chromatograms obtained for the identification of amino acids of various samples of pumpkin seeds under conditions of the procedure developed : 5,6,7, test solutions obtained from different series (RS 803, 804, 805); RS 4, solution of histidine, aspartic acid, glutamic acid, valine (in order of increasing Rf); RS 5, solution of arginine, glycine, alanine, and leucine (in order of increasing Rf)

Figure 4

Typical absorption spectra obtained by assay amino acids content: 1,2, spectra of the test solutions obtained from the series of pumpkin seeds; RS, spectrum of solution of glutamic acid
Typical absorption spectra obtained by assay amino acids content: 1,2, spectra of the test solutions obtained from the series of pumpkin seeds; RS, spectrum of solution of glutamic acid

Results of amino acids assay expressed as glutamic acid and fat-free raw material in the different samples of pumpkin seeds

RS (batches of pumpkin seeds)The content of the amino acids expressed as glutamic acid
7992.0 ± 0.05
8002.1 ± 0.03
8011.9 ± 0.04
8021.8 ± 0.05
8032.5± 0.07
8041.7 ± 0.03
8051.8 ± 0.04
Language:
English
Publication timeframe:
2 times per year
Journal Subjects:
Pharmacy, Pharmacy, other
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