<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>This study investig ated the activation of apoptosis and autophagy in CDV infected brain tissues of dogs with acute neurological signs, by determining Cas-3, Cas-8, Cas-9, Bax, Bcl-2, LC3B, and Beclin-1 expression with real-time PCR. The expression levels of Beclin-1 and LC3B, autophagy markers, were significantly up-regulated in comparison with the control group (p &lt; 0.001). The expression levels of apoptotic markers Cas-3, Cas-8, Cas-9 and Bax were slightly up-regulated, but Bcl-2 was slightly down-regulated in contrast to the control group (p &lt; 0.05). Therefore, the autophagy markers were more activated than apoptotic markers in dogs with acute neurological signs. In conclusion, autophagy takes part in the pathogenesis of demyelination in canine distemper. Knowing this may be helpful to create new therapeutic strategies, such as new effective antiviral medicines.</p>
</abstract>

This study investig ated the activation of apoptosis and autophagy in CDV infected brain tissues of dogs with acute neurological signs, by determining Cas-3, Cas-8, Cas-9, Bax, Bcl-2, LC3B, and Beclin-1 expression with real-time PCR. The expression levels of Beclin-1 and LC3B, autophagy markers, were significantly up-regulated in comparison with the control group (p < 0.001). The expression levels of apoptotic markers Cas-3, Cas-8, Cas-9 and Bax were slightly up-regulated, but Bcl-2 was slightly down-regulated in contrast to the control group (p < 0.05). Therefore, the autophagy markers were more activated than apoptotic markers in dogs with acute neurological signs. In conclusion, autophagy takes part in the pathogenesis of demyelination in canine distemper. Knowing this may be helpful to create new therapeutic strategies, such as new effective antiviral medicines.

Evaluation of Apoptosis and Autophagy Activities in the Brain of Dogs Naturally Infected with Canine Distemper Virus Based on Changes in Apoptotic and Autophagic Markers

Faculty of Veterinary Medicine, Department of Internal Medicine

Faculty of Veterinary Medicine, Department of Genetics

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

This study investig ated the activation of apoptosis and autophagy in CDV infected brain tissues of dogs with acute neurological signs, by determining Cas-3,...

Evaluation of Apoptosis and Autophagy Activities in the Brain of Dogs Naturally Infected with Canine Distemper Virus Based on Changes in Apoptotic and Autophagic Markers

Published Online: Jul 04, 2022

Page range: 195 - 206

Received: Dec 30, 2021

Accepted: May 04, 2022

DOI: https://doi.org/10.2478/acve-2022-0016

Keywords
apoptosis, autophagy, canine distemper, real-time PCR

© 2022 Şükrü Değirmençay et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Evaluation of Apoptosis and Autophagy Activities in the Brain of Dogs Naturally Infected with Canine Distemper Virus Based on Changes in Apoptotic and Autophagic Markers

Published Online: Jul 04, 2022

Page range: 195 - 206

Received: Dec 30, 2021

Accepted: May 04, 2022

DOI: https://doi.org/10.2478/acve-2022-0016

Keywordsapoptosis, autophagy, canine distemper, real-time PCR

© 2022 Şükrü Değirmençay et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
apoptosis, autophagy, canine distemper, real-time PCR