1. bookVolume 72 (2022): Issue 2 (June 2022)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Salmonella in Wild Boars (Sus Scrofa): Characterization and Epidemiology

Published Online: 04 Jul 2022
Volume & Issue: Volume 72 (2022) - Issue 2 (June 2022)
Page range: 184 - 194
Accepted: 23 May 2022
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

The large study on Salmonella spp. in the population of wild boars from twelve hunting estates in the South-West Vojvodina, Serbia was conducted with the aim to investigate the prevalence of Salmonella spp. in wild boars and to trace Salmonella sources. The hunting estates had similar epidemiological characteristics, i.e. lowland regions with an intensive management system of wild boars. The prevalence of Salmonella in wild boars was determined and the examination of molecular similarities of strains isolated from wild boars and domestic animals (pigs and poultry from nearby farms) was performed. The total number of 425 wild boars (25.3% of total population), shot on official hunts, were sampled (425 feces and 425 mesenteric lymph nodes samples) and examined by standard ISO protocols. Subtyping of the isolates was performed and compared by Pulsed-field gel electrophoresis (PFGE). The Salmonella prevalence in the fecal samples was 3.1% and in the lymph nodes was 0.2%. Salmonella Enteritidis was the most dominant serotype. A high molecular similarity was found between Salmonella isolates from wild boars and domestic animals. The proximity of communities and domestic animals, as well as improper removal of animal waste were identified as important epidemiological factors which significantly affect the epidemiology of Salmonella in wild boars from lowlands.

Keywords

1. Wisniewski J: The incidence of Salmonella spp. in wild boars in Poland. Poland Medycyna Wet 2001, 57(6):399-401. Search in Google Scholar

2. Wacheck S, Fredriksson-Ahomaa M, König M, Stolle A, Stephan R: Wild boars as an important reservoir for foodborne pathogens. Foodborne Pathog Dis 2010, 7(3):307-31210.1089/fpd.2009.036719899962 Search in Google Scholar

3. Paulsen P, Smulders F, Hilbert F: Salmonella in meat from hunted game: A central European perspective. Food Res Int 2012, 45(2):609-616.10.1016/j.foodres.2011.06.055 Search in Google Scholar

4. Navarro-Gonzalez N, Casas-Díaz E, Porrero CM, Mateos A, Domínguez L, Lavín S, Serrano E: Food-borne zoonotic pathogens and antimicrobial resistance of indicator bacteria in urban wild boars in Barcelona, Spain. Vet Microbiol 2013, 167(3-4):686-689.10.1016/j.vetmic.2013.07.03723992794 Search in Google Scholar

5. Peruzy MF, Murru N, Yu Z, Kerkhof PJ, Neola B, Joossens M, Proroga YTR, Houf K: Assessment of microbial communities on freshly killed wild boar meat by MALDI-TOF MS and 16S rRNA amplicon sequencing. Int J Food Microb 2019, 301:51-60.10.1016/j.ijfoodmicro.2019.05.00531100642 Search in Google Scholar

6. Orte ga N, Fanelli A, Serr anoa A, Mart inez-Carrasco C, Wscr ibano F, Tizz ani P, Cand ela M: Salmonella seroprevalence in wild boar from Southeast Spain depends on host population density. Res Vet Sci 2020, 132:400-40310.1016/j.rvsc.2020.07.02632763568 Search in Google Scholar

7. Mirceta J, Petrovic J, Malesevic M, Blagojevic B, Antic D: Assessment of microbial carcass contamination of hunted wild boars. Eur J Wildlife Res 2017 doi 10.1007/s10344-017-1096-3 Open DOISearch in Google Scholar

8. Cano-Manuel FJ, Lopez-Olvera J, Fandos P, Soriguer RC, Pérez JM, Granados JE: Long-term monitoring of 10 selected pathogens in wild boar (Sus scrofa) in Sierra Nevada national park, southern Spain. Vet Microbiol 2014, 174(1-2): 148-154.10.1016/j.vetmic.2014.06.01725261921 Search in Google Scholar

9. Díaz-Sánchez S, Sánchez S, Herrera-León S, Porrero C, Blanco J, Dahbi G, Blanco J, Mora A, Mateo R, Hanning I: Prevalence of Shiga toxin-producing Escherichia coli, Salmonella spp. and Campylobacter spp. in large game animals intended for consumption: relationship with management practices and livestock influence. Vet Microbiol 2013, 163(3-4):274-281.10.1016/j.vetmic.2012.12.02623384892 Search in Google Scholar

10. Polaček V, Mirčeta J, Prodanov-Radulović J: Key risk factors and impact of African Swine Fever spreading on pig production in Serbia. Acta Vet-Beograd 2021, 71(4):371-39110.2478/acve-2021-0032 Search in Google Scholar

11. ISO 6579:2002, Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. International Organization for Standardization. Search in Google Scholar

12. ISO 6579-3:2014, Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp.- Part 3: Guidelines for serotyping of Salmonella spp. International Organization for Standardization Search in Google Scholar

13. CDC: Standard operating procedure for PulseNet PFGE of Escherichia coli O157: H7, Escherichia coli non-O157 (STEC), Salmonella serotypes, Shigella sonnei and Shigella flexneri. Centers for Disease Control and Prevention, Atlanta. 2013. https://www.cdc.gov/pulsenet/pdf/ecoli-shigella-salmonella-pfge-protocol-508c.pdf Search in Google Scholar

14. Chiari M, Zanoni M, Tagliabue S, Lavazza A, Alborali LG: Salmonella serotypes in wild boars (Sus scrofa) hunted in northern Italy. Acta Vet Scand 2013, doi: 10.1186/1751-0147-55-42.366909923692883 Open DOISearch in Google Scholar

15. Vieira-Pinto M, Morais L, Caleja C, Themudo P, Torres C, Igrejas G, Poeta P, Martins C: Salmonella sp. in game (Sus scrofa and Oryctolagus cuniculus). Foodborne Pathog Dis 2011, 8(6):739-740.10.1089/fpd.2010.074221254910 Search in Google Scholar

16. Zottola T, Montagnaro S, Magnapera C, Sasso S, De Martino L, Bragagnolo A, D’Amici L, Condoleo R, Pisanelli G, Iovane G: Prevalence and antimicrobial susceptibility of Salmonella in European wild boar (Sus scrofa); Latium Region–Italy. Comp Immunol Microb 2013,.36(2):161-168.10.1016/j.cimid.2012.11.00423253890 Search in Google Scholar

17. Gill C: Microbiological conditions of meats from large game animals and birds. Meat Sci 2007, 77(2):149-160.10.1016/j.meatsci.2007.03.00722061585 Search in Google Scholar

18. Closa-Sebastià F, Casas-Díaz E, Cuenca R, Lavín S, Mentaberre G, Marco I: Antibodies to selected pathogens in wild boar (Sus scrofa) from Catalonia (NE Spain). Eur J Wildlife Res 2011, 57(4):977-981.10.1007/s10344-010-0491-9 Search in Google Scholar

19. Prodanov-Radulović J, Vučićević I, Polaček V, Aleksić-Kovačević S: Current swine respiratory diseases morphology in intensive swine production in Serbia. Acta Vet-Beograd 2020, 70(1):1-36.10.2478/acve-2020-0001 Search in Google Scholar

20. Stojanov I, Kapetanov M, Prodanov-Radulović J, Pušić I, Petrović J, Živkov-Baloš M.: The resistency of Salmonella servoar Enteritidis/Infantis isolated in poultry against nalidixic acid. Biotech anim husb 2011, 27(3):751-58.10.2298/BAH1103751S Search in Google Scholar

21. Velhner M, Potkonjak D, Stojanović D, Mitevski D, Stojanov I, Petrović J: Rezistencija salmonela na antibakterijske lekove i mere kontrole u živinarskoj proizvodnji.Vet glasn 2013, 67(1-2): 87-9610.2298/VETGL1302087V Search in Google Scholar

22. Petrovic J, Babic J, Stojanov I, Velhner M:The change in antimicrobial resistance profile of meat chain-associated Salmonella in Serbia. P Food Sci 2015, 5:231-234.10.1016/j.profoo.2015.09.059 Search in Google Scholar

23. Petrović J, Grgić Ž, Prodanov Radulović J, Ratajac R, Urošević M, Pustahija M, Medić S: Epidemiology of human trichinellosis in Vojvodina Province, Serbia, from 2015 to 2016. Acta Vet Hung 2019, 67(1):40–50 doiI: 10.1556/004.2019.00530922089 Open DOISearch in Google Scholar

24. Petrovic J, Grgic Z, Pusic I, Urosevic M: Sylvatic trichinellosis in the Vojvodina region (Serbia), in: Paulsen, P., Bauer, A., Vodnansky, M., Winkelmayer, R., Smulders, F.J.M. (Ed.), Trends in game meat hygiene. Wageningen Academic Publishers, Wageningen, 2014, 175-1.10.3920/978-90-8686-238-2_13 Search in Google Scholar

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