<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in horse plasma. Plasma samples were extracted with diethyl ether, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times were 3.0 min and 6.4 min for tildipirosin and tylosin tartrate, respectively. The total run time was 9 minutes in this method.</p>
<p>Calibration curves ranged from 0.1 to 3 μg/mL. The lower limit of detection for plasma was0.035μg/mL, and the lower limit of quantitation was 0.1 μg/mL. Both accuracy and precision were always &lt; 12% exce pt for LLOQ &lt; 20%. Mean recovery was 99.5 %. This procedure can be applied to determine tildipirosin concentrations in plasma and be useful to perform pharmacokinetic studies.</p>
</abstract>

A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in horse plasma. Plasma samples were extracted with diethyl ether, and after evaporation, tildipirosin was determined by reverse-phase chromatography with an ultraviolet detector set at a wavelength of 289 nm. Tildipirosin was separated on a Zorbax Eclipse XDB-C18 column, 150 x 3.0 mm, 5 μm with gradient chromatographic elution. The retention times were 3.0 min and 6.4 min for tildipirosin and tylosin tartrate, respectively. The total run time was 9 minutes in this method.
Calibration curves ranged from 0.1 to 3 μg/mL. The lower limit of detection for plasma was0.035μg/mL, and the lower limit of quantitation was 0.1 μg/mL. Both accuracy and precision were always < 12% exce pt for LLOQ < 20%. Mean recovery was 99.5 %. This procedure can be applied to determine tildipirosin concentrations in plasma and be useful to perform pharmacokinetic studies.

Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma

Department of Pharmacology, Faculty of Veterinary Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

A simple, rapid, low-cost, and sensitive high-performance liquid chromatographic method was developed to determine tildipirosin in horse plasma. Plasma samples...

Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma

Published Online: Apr 05, 2022

Page range: 100 - 110

Received: Sep 28, 2021

Accepted: Mar 08, 2022

DOI: https://doi.org/10.2478/acve-2022-0008

Keywords
horses, HPLC, plasma, Tildipirosin, validation

© 2022 Juan Sebastián Galecio et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Development and Validation of an Improved HPLC-UV Method for the Determination of Tildipirosin in Horse Plasma

Published Online: Apr 05, 2022

Page range: 100 - 110

Received: Sep 28, 2021

Accepted: Mar 08, 2022

DOI: https://doi.org/10.2478/acve-2022-0008

Keywordshorses, HPLC, plasma, Tildipirosin, validation

© 2022 Juan Sebastián Galecio et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
horses, HPLC, plasma, Tildipirosin, validation