<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay.</p>
</abstract>

The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay.

Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein

Department of Biochemistry and Immunology

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in...

Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein

Published Online: Mar 22, 2021

Page range: 98 - 106

Accepted: Dec 31, 2020

DOI: https://doi.org/10.2478/acve-2021-0007

Keywords
African Swine Fever Virus, Serotyping, EP402R gene, CD2v, Vietnam

© 2021 Tran Ha Thi Thanh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Rapid Identification for Serotyping of African Swine Fever Virus Based on the Short Fragment of the EP402R Gene Encoding for CD2-Like Protein

Published Online: Mar 22, 2021

Page range: 98 - 106

Accepted: Dec 31, 2020

DOI: https://doi.org/10.2478/acve-2021-0007

KeywordsAfrican Swine Fever Virus, Serotyping, EP402R gene, CD2v, Vietnam

© 2021 Tran Ha Thi Thanh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
African Swine Fever Virus, Serotyping, EP402R gene, CD2v, Vietnam