<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured calcium silicate cement (CS) and hydroxyapatite with calcium silicate cement (HA-CS) into root canals of rabbits’ teeth. The study was conducted on four rabbits of the genus Oryctolagus cuniculus. After instrumentation and irrigation, the root canals of the central incisors were dried and filled with CS, HA-CS and control material (MTA Angelus). The animals were sacrificed after 28 days. After histological preparation and hematoxylin-eosin staining, tissue samples were evaluated for the intensity and extension of inflammatory tissue reaction; continuity, morphology and thickness of the newly formed calcified tissue; and presence of giant cells, materials particles and microorganisms. Kruskal Wallis and Dunn’s post hoc test were used for data analysis (α=0.05). There were no significant differences in the intensity of inflammatory reactions between CS, HA-CS and MTA control. HA-CS showed significantly better results than MTA and CS with respect to continuity of the newly formed calcified tissue (P=0.003 and P=0.010, respectively). Significant differences in thickness of the calcified tissue existed between CS and MTA (P=0.004) and between HA-CS and MTA (P=0.012). Application of CS and HA-CS resulted in minimal inflammatory tissue response, similar to the MTA control. CS and HA-CS were more efficient than MTA in supporting hard tissue formation. The best organized newly formed calcified tissue was seen after HA-CS application.</p>
</abstract>

The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured calcium silicate cement (CS) and hydroxyapatite with calcium silicate cement (HA-CS) into root canals of rabbits’ teeth. The study was conducted on four rabbits of the genus Oryctolagus cuniculus. After instrumentation and irrigation, the root canals of the central incisors were dried and filled with CS, HA-CS and control material (MTA Angelus). The animals were sacrificed after 28 days. After histological preparation and hematoxylin-eosin staining, tissue samples were evaluated for the intensity and extension of inflammatory tissue reaction; continuity, morphology and thickness of the newly formed calcified tissue; and presence of giant cells, materials particles and microorganisms. Kruskal Wallis and Dunn’s post hoc test were used for data analysis (α=0.05). There were no significant differences in the intensity of inflammatory reactions between CS, HA-CS and MTA control. HA-CS showed significantly better results than MTA and CS with respect to continuity of the newly formed calcified tissue (P=0.003 and P=0.010, respectively). Significant differences in thickness of the calcified tissue existed between CS and MTA (P=0.004) and between HA-CS and MTA (P=0.012). Application of CS and HA-CS resulted in minimal inflammatory tissue response, similar to the MTA control. CS and HA-CS were more efficient than MTA in supporting hard tissue formation. The best organized newly formed calcified tissue was seen after HA-CS application.

Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations After Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements Into Root Canals of Rabbits Teeth

Department for Restorative Dentistry and Endodontics

Faculty of Veterinary Medicine, Department for Surgery, Orthopedic and Ophthalmology

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured...

Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations After Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements Into Root Canals of Rabbits Teeth

Published Online: Mar 22, 2021

Page range: 85 - 97

Received: Nov 30, 2019

Accepted: Dec 23, 2020

DOI: https://doi.org/10.2478/acve-2021-0006

Keywords
biocompatibility, calcium silicate, hard tissue formation, hydroxyapatite

© 2021 Petrović Violeta et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations After Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements Into Root Canals of Rabbits Teeth

Published Online: Mar 22, 2021

Page range: 85 - 97

Received: Nov 30, 2019

Accepted: Dec 23, 2020

DOI: https://doi.org/10.2478/acve-2021-0006

Keywordsbiocompatibility, calcium silicate, hard tissue formation, hydroxyapatite

© 2021 Petrović Violeta et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
biocompatibility, calcium silicate, hard tissue formation, hydroxyapatite