1. bookVolume 70 (2020): Issue 3 (September 2020)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

Entomological and Virological Methods for the Identification of Potential Vectors of Lumpy Skin Disease Virus in the South-Eastern Part of Northern Caucasus, Russia

Published Online: 18 Sep 2020
Volume & Issue: Volume 70 (2020) - Issue 3 (September 2020)
Page range: 296 - 304
Received: 27 Dec 2019
Accepted: 11 Jun 2020
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

The article provides assessment of field and laboratory methods for the collection and evaluation of potential vectors of lumpy skin disease virus (LSDV) in one of the districts of Krasnodarskiy Kray in southern Russia. In this study, we tested several methods of vector collection and a PCR protocol for the detection of the LSDV genome in insects. Descriptive data on samples were collected using a free web-based application Epicollect5.

Potential LSDV vectors are quite widely spread insects in this region. We identified 15 insect species, including Musca domestica, Musca autumnalis and Stomoxys calcitrans. Analysis of the insect population showed an increase in species diversity and a decrease in abundance of the insect population by the end of the flight season.

PCR tests did not detect LSDV genome in the collected samples. All the methods tested were found suitable for large-scale monitoring of lumpy skin disease (LSD). Further studies on potential risk factors of LSD spread are necessary to improve measures on preventing and eliminating the disease.

Keywords

1. Balysheva VI, Zhivoderov SP, Pivova EJ, Bobrovskaya NK, Zhivodeorova AV, Anisimova LI, Kushnir SD, Usadov TR, Yurkov SG, Salnikov NI, Lunitsin AV: Permissivity of cell cultures of different origin during the cultivation of lumpy skin disease virus. Agricultural Biology (Russian) 2017, 52 (6): 1265-1272.Search in Google Scholar

2. Konovalov MG, Shevchenko AA: Prevention of lumpy skin disease in the Krasnodar Territory. Proceedings of All-Russian Workshop “Aspects for new generation food production” dedicated to the memory of Professor GP Saprygin. PA Stolypin Omsk State Agriculture University 2017: 79-81.Search in Google Scholar

3. Order of the Ministry of Agriculture of Russian Federation No. 317 dated July 20, 2016.Search in Google Scholar

4. Chihota CM, Rennie LF, Kitching RP, Mellor PS: Attempted mechanical transmission of lumpy skin disease virus by biting insects. Med. Vet. Entomol. 2003, 17(3): 294-300.Search in Google Scholar

5. EFSA AHAW Panel (EFSA Panel on Animal Health and Welfare), 2015. Scientific Opinion on lumpy skin disease. EFSA Journal 2015,13(1):3986, 7310.2903/j.efsa.2015.3986Search in Google Scholar

6. Material from Wikipedia-free encyclopedia, Climatogram. Available online: [https://ru.wikipedia.org/wiki/Climate_Krasnodar].Search in Google Scholar

7. Podshibjakin DV, Blokhin AA: Guidelines for collecting, recording and conducting laboratory studies of Diptera for the genetic material of the lumpy skin disease virus using PCR. Volginsky, 2018.Search in Google Scholar

8. Tarbinskij SP, Plavilshchikov, NN: Key to insects of the European part of the USSR. OGIZ-SELKHOZGIZ, 1948.Search in Google Scholar

9. Bowden TR, Babiuk SL, Parkyn GR, Copps JS, Boyle DB: Capripoxvirus tissue tropism and shedding: A quantitative study in experimentally sick sheep and goats. Virology 2008, 371: 380–393.10.1016/j.virol.2007.10.00217988703Search in Google Scholar

10. Vidanović D, Šekler M, Petrović T, Debeljak Z, Vasković N, Matović K, Hoffmann B: Real-time PCR assays for the specific detection of field Balkan strains of lumpy skin disease virus. Acta Veterinaria-Beograd. 2016, 66 (4): 444-454.10.1515/acve-2016-0038Search in Google Scholar

11. Baldacchino F, Muenworn V, Desquesnes M, Desoli F, Charoenviriyaphap T, Duvallet G: Transmission of pathogens by Stomoxys flies (Diptera, Muscidae). Parasite 2013: 20:26.10.1051/parasite/2013026375633523985165Search in Google Scholar

12. Sohier C, Haegeman A, Mostin L, De Leeuw I, Van Campe W, De Vleeschauwer A, Tuppurainen ESM, van den Berg T, De Regge N, De Clercq K: Experimental evidence of mechanical lumpy skin disease virus transmission by Stomoxys calcitrans biting flies and Haematopota spp. horseflies. Sci Rep. 2019, 9(1):20076.10.1038/s41598-019-56605-6693483231882819Search in Google Scholar

13. Gubbins S: Using the basic reproduction number to assess the risk of transmission of lumpy skin disease virus by biting insects. Transbound Emerg Dis. 2019, 66(5):1873–1883.10.1111/tbed.13216676715731038286Search in Google Scholar

14. Sprygin A, Pestova Y, Prutnikov P, Kononov A: Detection of vaccine-like lumpy skin disease virus in cattle and Musca domestica L. flies in an outbreak of lumpy skin disease in Russia in 2017. Transbound Emerg Dis. 2018, 65(5):1137–1144.10.1111/tbed.1289729932512Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo