1. bookVolume 68 (2018): Issue 2 (June 2018)
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Open Access

Effect of Pasteurella multocida Soluble Antigen Stimulation on the In Vitro Response of Peripheral Blood Mononuclear Cells of Holstein Calves

Published Online: 04 Jul 2018
Volume & Issue: Volume 68 (2018) - Issue 2 (June 2018)
Page range: 201 - 210
Received: 05 Oct 2017
Accepted: 16 Mar 2018
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

The expressions of cytokines mRNA, including interleukin-4 (IL-4), interleukin- 17A (IL-17A) and interferon-gamma (IFN-γ), their master regulatory transcription factors, and signal transducers and activator of transcription (STAT) stimulated in vitro with Pasteurella (P.) multocida soluble antigen were examined in peripheral blood mononuclear cells (PBMC) from Holstein calves. The healthy Holstein calves were divided into three groups; 2 weeks old (2W Group, N=8), 6 weeks old (6W Group, N=8), and 10 weeks old (10W Group, N=8). PBMC were stimulated in vitro by soluble antigen of P. multocida. There were significantly lower expressions of IFN-γ, IL-4, and STAT-6 mRNA of PBMC stimulated with P. multocida soluble antigen in the 2W Group compared to that in the 10W Group. Expression of IL-17A and IFN-γ in PBMC stimulated with P. multocida soluble antigen were significantly higher compared with the PBMC without stimulation in the 6W groups. The results of the present study demonstrated that 2W old calves had decreased cytokine expression of PBMC when in vitro stimulated with P. multocida soluble antigen in vitro.

Keywords

1. Anderson G, Moore NC, Owen JJ, Jenkinson EJ. Cellular interactions in thymocyte development, Annual Rev Immunol 1996, 14:73-99.10.1146/annurev.immunol.14.1.73Search in Google Scholar

2. Holt PG, Jones CA. The development of the immune system during pregnancy and early life. Allergy 2000,55:88-897.10.1034/j.1398-9995.2000.00118.xSearch in Google Scholar

3. Adkins B, Leclerc C, and Marshall-Clarke S. Neonatal adaptive immunity comes of age. Nature Rev Immunol 2004,4:553–564.10.1038/nri1394Search in Google Scholar

4. Maródi L. Neonatal innate immunity to infectious agents. Inf Immun 2006, 74:1999-2006.10.1128/IAI.74.4.1999-2006.2006Search in Google Scholar

5. Mathy NL, Mathy J-PD, Lee RP, Walker J, Lofthouse S, Meeusen ENT. Pathological and immunological changes after challenge infection with Pasteurella multocida in naive and immunized calves. Vet Immunol Immunopathol 2002, 85:179-188.10.1016/S0165-2427(01)00427-5Search in Google Scholar

6. Reber AJ, Lockwood A, Hippen AR, Hurley DJ. Colostrum induced phenotypic and tracking changes in maternal mononuclear cells in a peripheral blood leukocyte model forvstudy of leukocyte transfer to the neonatal calf. Vet Immunol Immunopathol 2006, 109:139-50.10.1016/j.vetimm.2005.08.01416169602Search in Google Scholar

7. Ohtsuka H, Ono M, Saruyama Y, Mukai M, Kohiruimaki M, Kawamura S. Comparison of the peripheral blood leukocyte population between Japanese Black and Holstein calves. Anim Sci J 2011, 82:93-98.10.1111/j.1740-0929.2010.00833.x21269366Search in Google Scholar

8. Nagahata H, Kojima N, Higashitani J, Ogawa H, Noda H. Postnatal changes in lymphocyte function of dairy calves. Zentralbl Veterinarmed B 1991, 38:49-54.10.1111/j.1439-0450.1991.tb00845.x2063643Search in Google Scholar

9. DeBey BM, Roth JA, Brogden KA, Cutlip RC, Stevens MG, Jones T, Briggs RE, Kluge JP. In vitro lymphocyte proliferative responses and gamma-interferon production as measures of cell-mediated immunity of cattle exposed to Pasteurella haemolytica. Can J Vet Res 1996, 60:263-270.Search in Google Scholar

10. Jacks S, Giguère S, Crawford PC, Castleman WL. Experimental infection of neonatal foals with Rhodococcus equi triggers adult-like gamma interferon induction. Clin Vac Immunol 2007, 14:669-677.10.1128/CVI.00042-07195107217409222Search in Google Scholar

11. Maeda Y, Ohtsuka H, Tomioka M, Oikawa M. Effect of progesterone on Th1/Th2/Th17 and regulatory T cell-related genes in peripheral blood mononuclear cells during pregnancy in cows. Vet Res Commun 2013, 37:43-49.10.1007/s11259-012-9545-723203561Search in Google Scholar

12. Keski-Nisula L, Hirvonen MR, Roponen M, Heinonen S, Pekkanen J. Maternal and neonatal IL-4 and IFN-gamma production at delivery and 3 months after birth. J Reprod Immunol 2003,60:25-33.10.1016/S0165-0378(03)00079-2Search in Google Scholar

13. Baker SJ, Ran G, Reddy EP. Hematopoietic cytokine receptor signaling. Oncogene. 2007, 26:6724-6737.10.1038/sj.onc.121075717934481Search in Google Scholar

14. Maródi L, Goda K, Palicz A, Szabó G. Cytokine receptor signalling in neonatal macrophages: defective STAT-1 phosphorylation in response to stimulation with IFN-gamma. Clin Exp Immunol. 2001, 126:456-460.10.1046/j.1365-2249.2001.01693.x190623411737062Search in Google Scholar

15. Cardoso CR, Garlet GP, Crippa GE, Rosa AL, Junior WM, Rossi MA, Silva JS. Evidence of the presence of T helper type 17 cells in chronic lesions of human periodontal disease. Oral Micro Immunol 2009,24:1-6.10.1111/j.1399-302X.2008.00463.x19121062Search in Google Scholar

16. Volpe E, Servant N, Zollinger R, Bogiatzi SI, Hupé P, Barillot E, Soumelis V. A critical function for transforming growth factor-beta, interleukin 23 and proinflammatory cytokines in driving and modulating human T(H)-17 responses. Nature Immunol 2008, 9:650-657.10.1038/ni.161318454150Search in Google Scholar

17. Yeh W, McWilliams IL, Harrington LE. IFNγ inhibits Th17 differentiation and function via Tbet-dependent and Tbet-independent mechanisms. J Neuroimmunol. 2014, 267:20-27.10.1016/j.jneuroim.2013.12.001436399724369297Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo