<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The 2009 outbreak of H1N1 influenza A viruses in humans underscored the importance of pigs in influenza A virus evolution and the emergence of novel viruses with pandemic potential. In addition, influenza A virus infections continued to cause production losses in the agricultural industry resulting in a significant drop of profit. The primary method to control influenza A virus infections in pigs is through vaccination. Previously we demonstrated that two doses of an elastase-dependent live attenuated swine influenza virus administered by either the intratracheal or intranasal route can provide a high degree of protection in pigs against challenge with both homologous and different heterologous swine influenza viruses. Here we report the protection efficacy of a single dose elastase-dependent live attenuated swine influenza virus administered by the intranasal route against challenge with homologous subtypic H1N1 2009 pandemic swine-like influenza virus. Protection was observed in the absence of neutralizing antibodies specific for H1N1 2009 in sera.</p></abstract>

The 2009 outbreak of H1N1 influenza A viruses in humans underscored the importance of pigs in influenza A virus evolution and the emergence of novel viruses with pandemic potential. In addition, influenza A virus infections continued to cause production losses in the agricultural industry resulting in a significant drop of profit. The primary method to control influenza A virus infections in pigs is through vaccination. Previously we demonstrated that two doses of an elastase-dependent live attenuated swine influenza virus administered by either the intratracheal or intranasal route can provide a high degree of protection in pigs against challenge with both homologous and different heterologous swine influenza viruses. Here we report the protection efficacy of a single dose elastase-dependent live attenuated swine influenza virus administered by the intranasal route against challenge with homologous subtypic H1N1 2009 pandemic swine-like influenza virus. Protection was observed in the absence of neutralizing antibodies specific for H1N1 2009 in sera.

A single dose vaccination with an elastase-dependent H1N1 live attenuated swine influenza virus protects pigs from challenge with 2009 pandemic H1N1 virus

National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg MB, Canada; Bioniche Life Sciences Inc, 231 Dundas Street East Belleville, ON, Canada

National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg MB, Canada;

Vaccine & Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada;

National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg MB, Canada; Department of Immunology, University of Manitoba, Winnipeg MB, Canada;

Acta Veterinaria

The 2009 outbreak of H1N1 influenza A viruses in humans underscored the importance of pigs in influenza A virus evolution and the emergence of novel viruses...

A single dose vaccination with an elastase-dependent H1N1 live attenuated swine influenza virus protects pigs from challenge with 2009 pandemic H1N1 virus

Published Online: Mar 25, 2014

Page range: 10 - 23

DOI: https://doi.org/10.2478/acve-2014-0002

Keywords
H1N1, swine influenza, pandemic influenza, vaccine

This content is open access.

A single dose vaccination with an elastase-dependent H1N1 live attenuated swine influenza virus protects pigs from challenge with 2009 pandemic H1N1 virus

Published Online: Mar 25, 2014

Page range: 10 - 23

DOI: https://doi.org/10.2478/acve-2014-0002

KeywordsH1N1, swine influenza, pandemic influenza, vaccine

This content is open access.

Keywords
H1N1, swine influenza, pandemic influenza, vaccine