1. bookVolume 64 (2014): Issue 1 (March 2014)
Journal Details
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Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Open Access

Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts

Published Online: 25 Mar 2014
Volume & Issue: Volume 64 (2014) - Issue 1 (March 2014)
Page range: 24 - 32
Journal Details
License
Format
Journal
eISSN
1820-7448
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
Abstract

The objective of the study was to determine the concentration of BMP-15 and GDF-9 in the fluid of follicular cysts and ovarian follicles, and to compare their concentrations in mono- and polyoocyte follicles in gilts. The study involved two experiments conducted on the ovaries collected post-slaughter from gilts (7-8 months old). The first experiment covered 31 follicular single cyst gilts (15-25 mm in diameter) and 41 gilts without cysts. Follicular fluid from follicles of 8-10 mm in diameter (n=41) and 5-8 mm in diameter (n=41), and cystic fluid (n=31) were collected for analysis. The second experiment involved collecting follicular fluid from poly- (n=19) and monooocyte (n=22) follicles. The concentration of BMP-15 and GDF-9 was then determined in the samples using specimen-specific ELISA kits. The differences in the concentration of these factors were calculated by means of analysis of variance and a posthoc test. Duncan’s multiple range test was used to verify the significance of differences at P<0.05 and P<0.01. In addition, correlations between the factors were calculated. BMP-15 and GDF-9 levels in the cystic fluid were significantly higher than those in the follicular fluid (P<0.01). However, no differences were observed between various size follicles or between mono- and polyoocyte follicles. BMP-15 and GDF-9 concentrations were found to be positively correlated (P<0.01). Differences in BMP-15 and GDF-9 concentrations in ovarian follicles and follicular cysts, as evidenced by our study, indicate that these factors may be related to folliculogenesis disorders in gilts. What is more, the number of oocytes in ovarian follicles does not influence the intrafollicular concentration of BMP-15 and GDF-9.

Keywords

1. Knight PG, Glister C: TGF-β superfamily members and ovarian follicle development. Reproduction 2006, 132: 191-206.10.1530/rep.1.01074Search in Google Scholar

2. Su YQ, Sugiura K, Wigglesworth K, O’Brien MJ, Affourtit JP, Pangas SA, Matzuk MM, Eppig JJ: Oocyte regulation of metabolic cooperativity between mouse cumulus cells and oocytes: BMP-15 and GDF-9 control cholesterol biosynthesis in cumulus cells. Development 2008, 135: 111-121.10.1242/dev.009068Search in Google Scholar

3. Sun RZ, Lei L, Cheng L, Jin ZF, Zu SJ, Shan ZY, Wang ZD, Zhang JX, Liu ZH: Expression of GDF-9, BMP-15 and their receptors in mammalian ovary follicles. J Mol Hist 2010, 41: 325-332.10.1007/s10735-010-9294-2Search in Google Scholar

4. Orisaka M, Jiang JY, Orisaka S, Kotsuji F, Tsang BK: Growth differentiation factor 9 promotes rat preantral follicle growth by up-regulating follicular androgen biosynthesis. Endocrinology 2009, 150: 2740-2748.10.1210/en.2008-1536Search in Google Scholar

5. Peng X, Yang M, Wang L, Tong C, Guo Z: In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium. J Assist Reprod Genet 2010, 27: 247-257.10.1007/s10815-010-9415-6Search in Google Scholar

6. Su YQ, Wu X, O’Brien MJ, Pendola FL, Denegre JN, Matzuk MM Eppig JJ: Synergistic roles of BMP15 and GDF9 in the development and function of the oocyte-cumulus cell complex in mice: genetic evidence for an oocyte-granulosa cell regulatory loop. Dev Biol 2004, 27: 64-73.10.1016/j.ydbio.2004.08.020Search in Google Scholar

7. Paradis F, Novak S, Murdoch GK, Dyck MK, Dixon WT, Foxcroft GR: Temporal regulation of BMP2, BMP6, BMP15, GDF9, BMPR1A, MPR1B, BMPR2 and TGFBR1 mRNA expression in the oocyte, granulosa and theca cells of developing preovulatory follicles in the pig. Reproduction 2009, 138: 115-129.10.1530/REP-08-0538Search in Google Scholar

8. Stankiewicz T, Błaszczyk B, Udała J: Selected aspects of pig oocytes maturation in vivo and in vitro. Med Weter 2008, 64: 400-403 (in polish).Search in Google Scholar

9. Paulini F, Melo EO: The Role of Oocyte-Secreted Factors GDF9 and BMP15 in Follicular Development and Oogenesis. Reprod Dom Anim 2011, 46: 354-361.10.1111/j.1439-0531.2010.01739.xSearch in Google Scholar

10. Ebbert W, Bostedt H: Cystic Degeneration in porcine ovaries - fi rst communication: morphology of cystic ovaries, interpretation of the results. Reprod Dom Anim 1993, 28: 441-450.10.1111/j.1439-0531.1993.tb01021.xSearch in Google Scholar

11. Fitko RJ, Kucharski B, Szlezyngier B, Jana B: The concentration of GnRH in hypothalamus, LH and FSH in pituitary, LH, PRL and sex steroids in peripheral and ovarian venous plasma of hypo- and hyperthyroid, cysts-bearing gilts. Anim Reprod Sci 1996, 45: 123-138.10.1016/S0378-4320(96)01568-0Search in Google Scholar

12. Castagna CD, Peixoto CH, Bortolozzo FP, Wentz I, Neto GB, Ruschel F: Ovarian cysts and their consequences on the reproductive performance of swine herds. Anim Reprod Sci 2004, 81: 115-123.10.1016/j.anireprosci.2003.08.00414749053Search in Google Scholar

13. Cech S, Dolezel R: Treatment of ovarian cysts in sows - a fi eld trial. Vet Med-Czech 2007, 52, 413-418.10.17221/1995-VETMEDSearch in Google Scholar

14. Tummaruk P, Kesdangsakonwut S: Factors affecting the incidence of cystic ovaries in replacement gilts. Comp Clin Path 2012, 21: 1-7.10.1007/s00580-010-1055-9Search in Google Scholar

15. Ebbert WF, Elsaesser F, Bostedt H: Cystic Degeneration in Porcine Ovaries - Second Communication: Concentrations of Progesterone, Estradiol-17β, and Testosterone in Cystic Fluid and Plasma; Interpretation of the Results. Reprod Dom Anim 1993, 28: 451-463.10.1111/j.1439-0531.1993.tb01022.xSearch in Google Scholar

16. Safran A, Reubinoff BE, Porat-Katz A, Werner M, Friedler S, Lewin A: Intracytoplasmic sperm injection allows fertilization and development of a chromosomally balanced embryo from a binoovular zona pellucida. Hum Reprod 1998, 13: 2575-2578.10.1093/humrep/13.9.25759806286Search in Google Scholar

17. Payan-Carreira R, Pires MA: Multioocyte follicles in domestic dogs: a survey of frequency of occurrence. Theriogenology 2008, 69: 977-982.10.1016/j.theriogenology.2008.01.01318358525Search in Google Scholar

18. Stankiewicz T, Błaszczyk B, Udała J: A Study on the Occurrence of Polyovular Follicles in Porcine Ovaries with Particular Reference to Intrafollicular Hormone Concentrations, Quality of Oocytes and their in vitro Fertilization. Anat Histol Embryol 2009, 38: 233-239.10.1111/j.1439-0264.2009.00929.x19469770Search in Google Scholar

19. Hunter MG, Robinson RS, Mann GE, Webb R: Endocrine and paracrine control of follicular development and ovulation rate in farm species. Anim Reprod Sci 2004, 82-83: 461-477.10.1016/j.anireprosci.2004.05.01315271473Search in Google Scholar

20. Fouladi-Nashta AA, Waddington D, Campbell KHS: Maintenance of bovine oocytes in meiotic arrest and subsequent development in vitro: a comparative evaluation of antral follicle culture with other methods. Biol Reprod 1998, 59: 255-262.10.1095/biolreprod59.2.2559687293Search in Google Scholar

21. Eppig JJ: Oocyte control of ovarian follicular development and function in mammals. Reproduction 2001, 122: 829-838.10.1530/rep.0.122082911732978Search in Google Scholar

22. Otsuka F, Mctavish KJ, Shimasaki S: Integral Role of GDF-9 and BMP-15 in Ovarian Function. Mol Reprod Dev 2011, 78: 9-21.10.1002/mrd.21265305183921226076Search in Google Scholar

23. Prochazka R, Nemcova L, Nagyova E, Kanka J: Expression of Growth Differentiation Factor 9 Messenger RNA in Porcine Growing and Preovulatory Ovarian Follicles. Biol Reprod 2004, 71: 1290-1295.10.1095/biolreprod.104.02791215189836Search in Google Scholar

24. Sun YL, Zhang J, Ping ZG, Wang CQ, Sun YF, Chen L, Li XY, Li CJ, Zhu XL, Liu Z, Zhang W, Zhou X: Relationship Between Apoptosis and Proliferation in Granulosa and Theca Cells of Cystic Follicles in Sows. Reprod Dom Anim 2012, 47: 601-608.10.1111/j.1439-0531.2011.01929.x22023076Search in Google Scholar

25. Błaszczyk B, Udała J, Gączarzewicz D: Apoptosis in the atresia of ovarian follicles. Med Weter 2000, 56: 158-163 (in polish).Search in Google Scholar

26. Orisaka M, Orisaka S, Jiang JY, Craig J, Wang Y, Kotsuji F, Tsang BK: Growth differentiation factor 9 is antiapoptotic during follicular development from preantral to early antral stage. Mol Endocrinol 2006, 20: 2456-2468.10.1210/me.2005-035716740654Search in Google Scholar

27. Hu CL, Cowan RG, Harman RM, Quirk SM: Cell cycle progression and activation of Akt kinase are required for insulin-like growth factor I-mediated suppression of apoptosis in granulosa cells. Mol Endocrinol 2004, 18: 326-338.10.1210/me.2003-017814593075Search in Google Scholar

28. Craig J, Orisaka M, Wang H, Orisaka S, Thompson W, Zhu C, Kotsuji F, Tsang BK: Gonadotropin and intra-ovarian signals regulating follicle development and atresia: the delicate balance between life and death. Front Biosci 2007, 12: 3628-3639.10.2741/233917485326Search in Google Scholar

29. Solovyeva EV, Hayashi M, Margi K, Barkats C, Klein C, Amsterdam A, Hsueh AJ Tsafriri A: Growth Differentiation Factor-9 Stimulates Rat Theca-Interstitial Cell Androgen Biosynthesis. Biol Reprod 2000, 63: 1214-1218.10.1095/biolreprod63.4.121410993847Search in Google Scholar

30. Pache TD, Chadha S, Gooren LJ, Hop WC, Jaarsma KW, Dommerholt HB, Fauser BC: Ovarian morphology in long-term androgen-treated female to male transsexuals. A human model for the study of polycystic ovarian syndrome? Histopathology 1991, 19: 445-452.10.1111/j.1365-2559.1991.tb00235.x1757084Search in Google Scholar

31. Galloway SM, McNatty KP, Cambridge LM, Laitinen MPE, Juengel JL, Jokiranta S, McLaren RJ, Luiro K, Dodds KG, Montgomery GW, Beattie AE, Davis GH, Ritvos O: Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nat Genet 2000, 25: 279-283.10.1038/7703310888873Search in Google Scholar

32. Hanrahan JP, Gregan SM, Mulsant P, Mullen M, Davis GH, Powell R, Galloway SM: Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol Reprod 2004, 70: 900-909.10.1095/biolreprod.103.02309314627550Search in Google Scholar

33. Vireque AA, Reis RM, Rosa e Silva AAM, Resende LOT, Ferreira EM, Rosa e Silva ACJS, Ferriani RA: Involvement of Bone Morphogenetic Proteins (BMPs) in Ovarian Function and Infertility. Open Reprod Sci J 2008, 1: 11-15. 10.2174/1874255600801010011Search in Google Scholar

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