<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The topic of free radicals and related antioxidants is greatly discussed nowadays. Antioxidants help to neutralize free radicals before damaging cells. In the absence of antioxidants, a phenomenon called oxidative stress occurs. Oxidative stress can cause many diseases e.g. Alzheimer’s disease and cardiovascular diseases. Therefore, antioxidant activity of various compounds and the mechanism of their action have to be studied. Antioxidant activity and capacity are measured by <italic>in vitro</italic> and <italic>in vivo</italic> methods; <italic>in vitro</italic> methods are divided into two groups according to chemical reactions between free radicals and antioxidants. The first group is based on the transfer of hydrogen atoms (HAT), the second one on the transfer of electrons (ET). The most frequently used methods in the field of antioxidant power measurement are discussed in this work in terms of their principle, mechanism, methodology, the way of results evaluation and possible pitfalls.</p></abstract>

The topic of free radicals and related antioxidants is greatly discussed nowadays. Antioxidants help to neutralize free radicals before damaging cells. In the absence of antioxidants, a phenomenon called oxidative stress occurs. Oxidative stress can cause many diseases e.g. Alzheimer’s disease and cardiovascular diseases. Therefore, antioxidant activity of various compounds and the mechanism of their action have to be studied. Antioxidant activity and capacity are measured by in vitro and in vivo methods; in vitro methods are divided into two groups according to chemical reactions between free radicals and antioxidants. The first group is based on the transfer of hydrogen atoms (HAT), the second one on the transfer of electrons (ET). The most frequently used methods in the field of antioxidant power measurement are discussed in this work in terms of their principle, mechanism, methodology, the way of results evaluation and possible pitfalls.

Selected<italic>in vitro</italic> methods to determine antioxidant activity of hydrophilic/lipophilic substances

Slovak University of Technology in Bratislava

Faculty of Chemical and Food Technology, Institute of Food Science and Nutrition, Department of Food Technology

Acta Chimica Slovaca

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Selected methods to determine antioxidant activity of hydrophilic/lipophilic substances

The topic of free radicals and related antioxidants is greatly discussed nowadays. Antioxidants help to neutralize free radicals before damaging cells. In the...

Selectedin vitro methods to determine antioxidant activity of hydrophilic/lipophilic substances

Published Online: Jan 21, 2020

Page range: 200 - 211

DOI: https://doi.org/10.2478/acs-2019-0028

Keywords
ET methods, HAT methods, oxidative stress, total antioxidant activity

© 2019 Aneta Ácsová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Selectedin vitro methods to determine antioxidant activity of hydrophilic/lipophilic substances

Published Online: Jan 21, 2020

Page range: 200 - 211

DOI: https://doi.org/10.2478/acs-2019-0028

KeywordsET methods, HAT methods, oxidative stress, total antioxidant activity

© 2019 Aneta Ácsová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
ET methods, HAT methods, oxidative stress, total antioxidant activity