1. bookVolume 72 (2022): Issue 1 (March 2022)
Journal Details
License
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Journal
First Published
28 Feb 2007
Publication timeframe
4 times per year
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English
access type Open Access

Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons

Published Online: 30 Aug 2021
Page range: 123 - 134
Accepted: 13 Jan 2021
Journal Details
License
Format
Journal
First Published
28 Feb 2007
Publication timeframe
4 times per year
Languages
English
Abstract

In this study, the neuroprotective potential of arbutin (100 µmol L−1) pre-treatment and post-treatment against oxygen/ glucose deprivation (OGD) and reoxygenation (R) induced ischemic injury in cultured rat cortical neurons was explored. The OGD (60 min) and reoxygenation (24 h) treatment significantly (p < 0.001) compromised the antioxidant defence in cultured neurons. Subsequently, an increase (p < 0.001) in lipid peroxidation and inflammatory cytokines (tumour necrosis factor-α and nuclear factor kappa-B) declined neuron survival. In pre- and post-condition experiments, treatment with arbutin enhanced both survival (p < 0.01) and integrity (p < 0.05) of cultured neurons. Results showed that arbutin protects (p < 0.05) against peroxidative changes, inflammation, and enhanced the antioxidant activity (e.g., glutathione, superoxide dismutase and catalase) in cultured neurons subjected to OGD/R. It can be inferred that arbutin could protect against ischemic injuries and stroke. The anti-ischemic activity of arbutin can arrest post-stroke damage to the brain.

Keywords

1. Z. Yang, C. Weian, H. Susu and W. Hanmin, Protective effects of mangiferin on cerebral ischemiareperfusion injury and its mechanisms, Eur. J. Pharmacol. 771 (2015) 145–151; https://doi.org/10.1016/j.ejphar.2015.12.003 Search in Google Scholar

2. R. H. Lee, M. H. Lee, C. Y. Wu, A. Couto e Silva, H. E. Possoit, T. H. Hsieh, A. Minagar and H. W. Lin, Cerebral ischemia and neuroregeneration, Neural Regen. Res. 13 (2018) 373–385; https://doi.org/10.4103/1673-5374.228711 Search in Google Scholar

3. W. Li and S. Yang, Targeting oxidative stress for the treatment of ischemic stroke: Upstream and downstream therapeutic strategies, Brain Circ. 2 (2016) 153–163; https://doi.org/10.4103/2394-8108.195279 Search in Google Scholar

4. R. L. Jayaraj, S. Azimullah, R. Beiram, F. Y. Jalal and G. A. Rosenberg, Neuroinflammation: friend and foe for ischemic stroke, J. Neuroinflammation 16 (2019) Article ID 142 (24 pages); https://doi.org/10.1186/s12974-019-1516-2 Search in Google Scholar

5. D. Lin, L. Wang, S. Yan, Q. Zhang, J. H. Zhang and A. Shao, The role of oxidative stress in common risk factors and mechanisms of cardio-cerebrovascular ischemia and depression, Oxid. Med. Cell. Longev. 2019 (2019) Article ID 2491927 (13 pages); https://doi.org/10.1155/2019/2491927 Search in Google Scholar

6. O. A. Harari and J. K. Liao, NF-κB and innate immunity in ischemic stroke, Ann. N. Y. Acad. Sci. 1207 (2010) 32–40; https://doi.org/10.1111/j.1749-6632.2010.05735.x Search in Google Scholar

7. M. Kawabori and M. A. Yenari, Inflammatory responses in brain ischemia, Curr. Med. Chem. 22 (2015) 1258–1277; https://doi.org/10.2174/0929867322666150209154036 Search in Google Scholar

8. T. Shichita, M. Ito and A. Yoshimura, Post-ischemic inflammation regulates neural damage and protection, Front. Cell. Neurosci. 8 (2014) Article ID 319 (8 pages); https://doi.org/10.3389/fncel.2014.00319 Search in Google Scholar

9. C. Pop, L. Vlase and M. Tamas, Natural resources containing arbutin. Determination of arbutin in the leaves of Bergenia crassifolia (L.) Fritsch. acclimated in Romania, Not. Bot. Hortic. Agrobot. Cluj-Napoca 37 (2009) 129–132; https://doi.org/10.15835/nbha3713108 Search in Google Scholar

10. F. Yousefi, S. Mahjoub, M. Pouramir and F. Khadir, Hypoglycemic activity of Pyrus biossieriana Buhse leaf extract and arbutin: Inhibitory effects on alpha amylase and alpha glucosidase, Casp. J. Int. Med. 4 (2013) 763–767. Search in Google Scholar

11. F. Khadir, M. Pouramir, S. G. Joorsaraee, F. Feizi, H. Sorkhi and F. Yousefi, The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats, Casp. J. Int. Med. 6 (2015) 196–200. Search in Google Scholar

12. M. E. Shahaboddin, M. Pouramir, A. A. Moghadamnia, H. Parsian, M. Lakzaei and H.Mir, Pyrus biossieriana Buhse leaf extract: An antioxidant, antihyperglycaemic and antihyperlipidemic agent, Food Chem. 126 (2011) 1730–1733; https://doi.org/10.1016/j.foodchem.2010.12.069 Search in Google Scholar

13. B. E. Myagmar, E. Shinno, T. Ichiba and Y. Aniya, Antioxidant activity of medicinal herb Rhodococcum vitis-idaea on galactosamine-induced liver injury in rats, Phytomedicine 11 (2004) 416–423; https://doi.org/10.1016/j.phymed.2003.04.003 Search in Google Scholar

14. S. R. Ahmadian, M. Ghasemi-Kasman, M. Pouramir and F. Sadeghi, Arbutin attenuates cognitive impairment and inflammatory response in pentylenetetrazol-induced kindling model of epilepsy, Neuropharmacology 146 (2019) 117–127; https://doi.org/10.1016/j.neuropharm.2018.11.038 Search in Google Scholar

15. H. J. Lee and K. W. Kim, Anti–inflammatory effects of arbutin in lipopolysaccharide-stimulated BV2 microglial cells, Inflamm. Res. 61 (2012) 817–825; https://doi.org/10.1007/s00011-012-0474-2 Search in Google Scholar

16. M. Dadgar, M. Pouramir, Z. Dastan, M. Ghasemi-Kasman, M. Ashrafpour, A. A. Moghadamnia, S. Khafri and M. Pourghasem, Arbutin attenuates behavioral impairment and oxidative stress in an animal model of Parkinson’s disease, Avicenna J. Phytomed. 8 (2018) 533–542. Search in Google Scholar

17. Y. Ding, D. Kong, T. Zhou, N.-D. Yang, C. Xin, J. Xu, Q. Wang, H. Zhang, Q. Wu, X. Lu, K. Lim, B. Ma, C. Zhang, L. Li and W. Huang, α-Arbutin protects against Parkinson’s disease-associated mitochondrial dysfunction in vitro and in vivo, Neuromol. Med. 22 (2019) 56–67; https://doi.org/10.1007/s12017-019-08562-6 Search in Google Scholar

18. Z. Dastan, M. Pouramir, M. Ghasemi-Kasman, Z. Ghasemzadeh, M. Dadgar, M. Gol, M. Ashraf-pour, M. Pourghasem, A. A. Moghadamnia and S. Khafri, Arbutin reduces cognitive deficit and oxidative stress in animal model of Alzheimer’s disease, Int. J. Neurosci. 129 (2019) 1145–1153; https://doi.org/10.1080/00207454.2019.1638376 Search in Google Scholar

19. J. X. Wu, L. Y. Zhang, Y. L. Chen, S. S. Yu, Y. Zhao and J. Zhao, Curcumin pretreatment and post-treatment both improve the antioxidative ability of neurons with oxygen-glucose deprivation, Neural. Regen. Res. 10 (2015) 481–489; https://doi.org/10.4103/1673-5374.153700 Search in Google Scholar

20. J. Ye and Y. Zhang, Curcumin protects against intracellular amyloid toxicity in rat primary neurons, Int. J. Clin. Exp. Med. 5 (2012) 44–49. Search in Google Scholar

21. L. Wang, L. Zhang, Z. B. Chen, J. Y. Wu, X. Zhang and Y. Xu, Icariin enhances neuronal survival after oxygen and glucose deprivation by increasing SIRT1, Eur. J. Pharmacol. 609 (2009) 40–44; https://doi.org/10.1016/j.ejphar.2009.03.033 Search in Google Scholar

22. J. Xiang, Y. P. Tang, Z. Y. Zhou, P. Wu, Z. Wang, M. Mori and D. F. Cai, Apocynum venetum leaf extract protects rat cortical neurons from injury induced by oxygen and glucose deprivation in vitro, Can. J. Physiol. Pharmacol. 88 (2010) 907–917; https://doi.org/10.1139/y10-069 Search in Google Scholar

23. W. Zhao, S. Wang, T. Qin and W. Wang, Arbutin attenuates hydrogen peroxide-induced oxidative injury through regulation of microRNA-29a in retinal ganglion cells, Biomed. Pharmacother. 112 (2019) Article ID 108729; https://doi.org/10.1016/j.biopha.2019.108729 Search in Google Scholar

24. D. Gao, T. Huang, X. Jiang, S. Hu, L. Zhang and Z. Fei, Resveratrol protects primary cortical neuron cultures from transient oxygen-glucose deprivation by inhibiting MMP-9, Mol. Med. Rep. 9 (2014) 2197–2204; https://doi.org/10.3892/mmr.2014.2086 Search in Google Scholar

25. J. Wu, Q. Li, X. Wang, S. Yu, L. Li, X. Wu, Y. Chen, J. Zhao and Y. Zhao, Neuroprotection by curcumin in ischemic brain injury involves the Akt/Nrf2 pathway, PLoS ONE 8 (2013) e59843 (9 pages); https://doi.org/10.1371/journal.pone.0059843 Search in Google Scholar

26. R. AlJohri, M. AlOkail and S. H. Haq, Neuroprotective role of vitamin D in primary neuronal cortical culture, eNeurologicalSci 14 (2018) 43–48; https://doi.org/10.1016/j.ensci.2018.12.004 Search in Google Scholar

27. J. Chen-Roetling, L. Chen and R. F. Regan, Minocycline attenuates iron neurotoxicity in cortical cell cultures, Biochem. Biophys. Res. Commun. 386 (2009) 322–326; https://doi.org/10.1016/j.bbrc.2009.06.026 Search in Google Scholar

28. P. B. Godkar, R. K. Gordon, A. Ravindran and B. P. Doctor, Celastrus paniculatus seed oil and organic extracts attenuate hydrogen peroxide- and glutamate-induced injury in embryonic rat fore-brain neuronal cells, Phytomedicine 13 (2006) 29–36; https://doi.org/10.1016/j.phymed.2003.11.011 Search in Google Scholar

29. M. G. Ryou and R. T. Mallet, An in vitro oxygen-glucose deprivation model for studying ischemiareperfusion injury of neuronal cells, Methods Mol. Biol. 1717 (2018) 229–235; https://doi.org/10.1007/978-1-4939-7526-6_18 Search in Google Scholar

30. T. S. Anthonymuthu, E. M. Kenny and H. Bayır, Therapies targeting lipid peroxidation in traumatic brain injury, Brain Res. 1640 (2016) 57–76; https://doi.org/10.1016/j.brainres.2016.02.006 Search in Google Scholar

31. A. Nurmi, P. J. Lindsberg, M. Koistinaho, W. Zhang, E. Juettler, M. L. Karjalainen-Lindsberg, F. Weih, N. Frank, M. Schwaninger and J. Koistinaho, Nuclear factor-kappaB contributes to infarction after permanent focal ischemia, Stroke 35 (2004) 987–991; https://doi.org/10.1161/01.STR.0000120732.45951.26 Search in Google Scholar

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