<abstract xmlns="http://www.w3.org/1999/xhtml">The present study’s objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid is stable for up to 48 h and is localized in both cytoplasm and nucleus. It dose-dependently inhibited the viability of CRC cell lines; SW-620 and HT-29 with <italic>IC</italic>50 values of 7.2 ± 0.8 and 37.6 ± 1.4 µmol L–1. Besides, metastatic, invasive, and colony formation properties of CRC cells were significantly inhibited following the tannic acid treatment (<italic>p</italic> &lt; 0.001). Tannic acid has been found to modulate enzyme, protein, and gene expressions of NQO1 in different levels and the upregulation of protein/gene expressions of p53 (<italic>p</italic> &lt; 0.001), which leads the cells to trigger apoptosis. In conclusion, the present <italic>in vitro</italic> study may supply a significant background for <italic>in vivo</italic> studies in which the molecular mechanisms of antioxidant and chemopreventive activities of tannic acid will completely clarify.</abstract>

The present study’s objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid is stable for up to 48 h and is localized in both cytoplasm and nucleus. It dose-dependently inhibited the viability of CRC cell lines; SW-620 and HT-29 with IC50 values of 7.2 ± 0.8 and 37.6 ± 1.4 µmol L–1. Besides, metastatic, invasive, and colony formation properties of CRC cells were significantly inhibited following the tannic acid treatment (p < 0.001). Tannic acid has been found to modulate enzyme, protein, and gene expressions of NQO1 in different levels and the upregulation of protein/gene expressions of p53 (p < 0.001), which leads the cells to trigger apoptosis. In conclusion, the present in vitro study may supply a significant background for in vivo studies in which the molecular mechanisms of antioxidant and chemopreventive activities of tannic acid will completely clarify.

Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma

Faculty of Science, Department of Biochemistry

Ceyhan Faculty of Veterinary Medicine, Department of Physiology

Faculty of Engineering, Department of Biomedical Engineering

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The present study’s objective is to clarify the molecular mechanisms of tannic acid effects on the viability of human colorectal carcinoma (CRC). Tannic acid...

Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma

Published Online: Apr 03, 2021

Page range: 587 - 602

Accepted: Oct 27, 2020

DOI: https://doi.org/10.2478/acph-2021-0036

Keywords
tannic acid, colorectal carcinoma, wound healing, cell viability, NQO1, p53

© 2021 Serdar Karakurt et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Upregulation of p53 by tannic acid treatment suppresses the proliferation of human colorectal carcinoma

Published Online: Apr 03, 2021

Page range: 587 - 602

Accepted: Oct 27, 2020

DOI: https://doi.org/10.2478/acph-2021-0036

Keywordstannic acid, colorectal carcinoma, wound healing, cell viability, NQO1, p53

© 2021 Serdar Karakurt et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
tannic acid, colorectal carcinoma, wound healing, cell viability, NQO1, p53