Miniaturized shake-flask HPLC method for determination of distribution coefficient of drugs used in inflammatory bowel diseases
Published Online: Oct 21, 2019
Page range: 649 - 660
Accepted: Jun 09, 2019
DOI: https://doi.org/10.2478/acph-2019-0046
Keywords
© 2019 Edvin Brusač et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
A new method for determination of distribution coefficient of drugs azathioprine, 6-mercaptopurine and 6-thioguanine and nutrient folic acid used in the treatment of inflammatory bowel disease based on a miniaturized shake-flask and HPLC/DAD was developed. Special attention was made to the most commonly reported problems in the measurement of distribution coefficients using a shake-flask method such as mixing technique, speed and time, the temperature of experiment, type of buffer and its pH as well as